Co-immobilization of glucose oxidase and catalase for enhancing the performance of a membraneless glucose biofuel cell operated under physiological conditions

Nanoscale ◽  
2017 ◽  
Vol 9 (5) ◽  
pp. 1993-2002 ◽  
Author(s):  
Marcelinus Christwardana ◽  
Yongjin Chung ◽  
Yongchai Kwon
2011 ◽  
Vol 109 (2) ◽  
pp. 318-324 ◽  
Author(s):  
Mike Fischback ◽  
Ki Young Kwon ◽  
Inseon Lee ◽  
Su Jeong Shin ◽  
Hyun Gyu Park ◽  
...  

Biosensors ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 114 ◽  
Author(s):  
Brandon G. Roy ◽  
Julia L. Rutherford ◽  
Anna E. Weaver ◽  
Kevin Beaver ◽  
Michelle Rasmussen

Glutathione is an important biological molecule which can be an indicator of numerous diseases. A method for self-powered detection of glutathione levels in solution has been developed using an enzymatic biofuel cell. The device consists of a glucose oxidase anode and a bilirubin oxidase cathode. For the detection of glutathione, the inhibition of bilirubin oxidase leads to a measurable decrease in current and power output. The reported method has a detection limit of 0.043 mM and a linear range up to 1.7 mM. Being able to detect a range of concentrations can be useful in evaluating a patient’s health. This method has the potential to be implemented as a quick, low-cost alternative to previously reported methods.


Polymers ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 319 ◽  
Author(s):  
Kentaro Yoshida ◽  
Yu Kashimura ◽  
Toshio Kamijo ◽  
Tetsuya Ono ◽  
Takenori Dairaku ◽  
...  

Glucose-sensitive films were prepared through the layer-by-layer (LbL) deposition of hemin-modified poly(ethyleneimine) (H-PEI) solution and DNA solution (containing glucose oxidase (GOx)). H-PEI/DNA + GOx multilayer films were constructed using electrostatic interactions. The (H-PEI/DNA + GOx)5 film was then partially decomposed by hydrogen peroxide (H2O2). The mechanism for the decomposition of the LbL film was considered to involve more reactive oxygen species (ROS) that were formed by the reaction of hemin and H2O2, which then caused nonspecific DNA cleavage. In addition, GOx present in the LbL films reacts with glucose to generate hydrogen peroxide. Therefore, decomposition of the (H-PEI/DNA + GOx)5 film was observed when the thin film was immersed in a glucose solution. (H-PEI/DNA + GOx)5 films exposed to a glucose solution for periods of 24, 48 72, and 96 h indicated that the decomposition of the film increased with the time to 9.97%, 16.3%, 23.1%, and 30.5%, respectively. The rate of LbL film decomposition increased with the glucose concentration. At pH and ionic strengths close to physiological conditions, it was possible to slowly decompose the LbL film at low glucose concentrations of 1–10 mM.


1999 ◽  
Vol 23 (5) ◽  
pp. 481-487 ◽  
Author(s):  
Eugenii Katz ◽  
Boris Filanovsky ◽  
Itamar Willner
Keyword(s):  

2015 ◽  
Vol 264 ◽  
pp. 165-173 ◽  
Author(s):  
Arunas Ramanavicius ◽  
Asta Kausaite-Minkstimiene ◽  
Inga Morkvenaite-Vilkonciene ◽  
Povilas Genys ◽  
Raisa Mikhailova ◽  
...  

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