scholarly journals Mito-magneto: a tool for nanoparticle mediated mitochondria isolation

Nanoscale ◽  
2016 ◽  
Vol 8 (47) ◽  
pp. 19581-19591 ◽  
Author(s):  
Bhabatosh Banik ◽  
Brett W. Askins ◽  
Shanta Dhar
Keyword(s):  
Mitochondria Isolation

Large-scale purification procedure of spinach leaf mitochondria -isolation and immunological studies of the F1-ATPase

1990 ◽  
Vol 78 (3) ◽  
pp. 367-373 ◽  
Author(s):  
Beston Hamasur ◽  
Ulf Birgersson ◽  
AnnaCarin Eriksson ◽  
Elzbieta Glaser
Keyword(s):  
Large Scale ◽  
Purification Procedure ◽  
F1 Atpase ◽  
Spinach Leaf ◽  
Mitochondria Isolation

A critical comparison between two classical and a kit-based method for mitochondria isolation

PROTEOMICS ◽  
2009 ◽  
Vol 9 (11) ◽  
pp. 3209-3214 ◽  
Author(s):  
Sonja Hartwig ◽  
Christian Feckler ◽  
Stefan Lehr ◽  
Katrin Wallbrecht ◽  
Heike Wolgast ◽  
...  
Keyword(s):  
Critical Comparison ◽  
Mitochondria Isolation

New method of plant mitochondria isolation and sub-fractionation for proteomic analyses

Plant Science ◽  
2004 ◽  
Vol 167 (3) ◽  
pp. 389-395 ◽  
Author(s):  
Tomáš Hájek ◽  
David Honys ◽  
Věra Čapková
Keyword(s):  
Plant Mitochondria ◽  
New Method ◽  
Proteomic Analyses ◽  
Mitochondria Isolation

scholarly journals A versatile mitochondria isolation- and analysis-pipeline generates 3D nano-topographies and mechano-physical surface maps of single organelles

2021 ◽  
Author(s):  
Saurabh Joshi ◽  
Friederike Hater ◽  
Jürgen Eirich ◽  
Joakim Palovaara ◽  
Henrik Ellinghaus ◽  
...  
Keyword(s):  
Physical Properties ◽  
Redox Homeostasis ◽  
Biological Significance ◽  
Analysis Pipeline ◽  
Force Microscopy ◽  
Atomic Force ◽  
Versatile Tool ◽  
Associated Proteins ◽  
Analysis Platform ◽  
Mitochondria Isolation

Living eukaryotic cells typically contain large quantities of highly dynamic mitochondria, which sustain the cells energy and redox homeostasis. Growing evidence suggests that mitochondria can functionally differ among but also within cells. The extent and biological significance of mitochondrial diversity is still largely unexplored, due to technical limitations that hamper profiling of individual organelles. Previous measurements of the cells interior have shown that membrane-bound compartments respond to metabolic manipulation by changes in their surface stiffness, suggesting that mechano-physical properties are a valuable readout of mitochondrial function. We here present the establishment of a robust multi-step analysis pipeline that allows one to profile mechano-physical properties of single mitochondria at the nanoscale using Atomic Force Microscopy (AFM). Firstly, we developed a rapid cell-type specific isolation protocol (mRACE), which selectively functionalizes mitochondria with biotin, facilitating isolation by streptavidin decorated microbeads. We established the technique for human and rat cell cultures, the invertebrate Caenorhabditis elegans, and the model plant Arabidopsis thaliana. Based on this versatile tool, we detected diversity of mitochondrially associated proteins among different tissues, reflecting the trophic condition of the source material. Secondly, a rapid filtration-based mitochondria isolation protocol was established, which was combined with mRACE. Lastly, we established an AFM analysis platform, which generates 3D maps of the nano-topography and mechano-physical properties of individual mitochondria. The comparison of mitochondria with each other revealed an unprecedented diversity in their mechano-physical properties and suggests that shape is not the sole determining parameter for outer membrane stiffness. We expect our results to not only introduce a new dimension for basic mitochondrial research, but in addition to open the door for the exploitation of individual mitochondria for diagnostic characterization.

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