Investigating the DMPO-formate spin trapping method for the study of paper iron gall ink corrosion

2016 ◽  
Vol 40 (11) ◽  
pp. 9098-9110 ◽  
Author(s):  
Alice Gimat ◽  
Valeryia Kasneryk ◽  
Anne-Laurence Dupont ◽  
Sabrina Paris ◽  
Frédéric Averseng ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Hydroxyl Radicals ◽  
Reactive Oxygen ◽  
Spin Trapping ◽  
Oxygen Species ◽  
Paper Degradation ◽  
Trapping Method ◽  
Iron Gall

Reactive oxygen species evidenced in acidic iron gall inks are not hydroxyl radicals and are not linked to paper degradation.

Photocatalytic Degradation of 4-Chlorophenol by •OH Radicals Generated by Thiophene Oligomers Incorporated in ZSM-5 Zeolite Channels

2003 ◽  
Vol 68 (11) ◽  
pp. 2219-2230 ◽  
Author(s):  
Gabriel Čík ◽  
Milada Hubinová ◽  
František Šeršeň ◽  
Jozef Krištín ◽  
Monika Antošová
Keyword(s):  
Reactive Oxygen Species ◽  
Visible Light ◽  
Photocatalytic Degradation ◽  
Maleic Acid ◽  
Aqueous Suspension ◽  
Reactive Oxygen ◽  
Spin Trapping ◽  
Oh Radicals ◽  
Oxygen Species ◽  
Trapping Method

Degradation of 4-chlorophenol by reactive oxygen species was studied, the latter being generated by photo-assisted reactions of thiophene oligomers, synthesized in channels of the Na-ZSM-5 zeolite. The photoreaction was carried out in an aqueous suspension of photocatalyst, irradiated with visible light (λ > 400 nm). The spin-trapping method was used to detect the generated •OH radicals. The main products of the photodecomposition of 4-chlorophenol were found to be phenol, hydroquinone and maleic acid.

scholarly journals Evaluation of the reactive oxygen species scavenging abilities of tomato juice using ESR spin trapping method

2017 ◽  
Vol 27 (4) ◽  
pp. 267-272 ◽  
Author(s):  
Hiromi Kameya ◽  
Toshihiko Shoji ◽  
Yuji Otagiri ◽  
Akio Obata ◽  
Vipavee Trivittayasil ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Spin Trapping ◽  
Tomato Juice ◽  
Oxygen Species ◽  
Esr Spin Trapping ◽  
Trapping Method

scholarly journals Role of mitochondrial superoxide dismutase in contraction-induced generation of reactive oxygen species in skeletal muscle extracellular space

2004 ◽  
Vol 286 (5) ◽  
pp. C1152-C1158 ◽  
Author(s):  
A. McArdle ◽  
J. van der Meulen ◽  
G. L. Close ◽  
D. Pattwell ◽  
H. Van Remmen ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Hydroxyl Radical ◽  
Hydroxyl Radicals ◽  
Extracellular Space ◽  
Superoxide Anion ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Superoxide Anions ◽  
Wild Type ◽  
Mnsod Activity

Contractions of skeletal muscles produce increases in concentrations of superoxide anions and activity of hydroxyl radicals in the extracellular space. The sources of these reactive oxygen species are not clear. We tested the hypothesis that, after a demanding isometric contraction protocol, the major source of superoxide and hydroxyl radical activity in the extracellular space of muscles is mitochondrial generation of superoxide anions and that, with a reduction in MnSOD activity, concentration of superoxide anions in the extracellular space is unchanged but concentration of hydroxyl radicals is decreased. For gastrocnemius muscles from adult (6–8 mo old) wild-type ( Sod2+/+) mice and knockout mice heterozygous for the MnSOD gene ( Sod2+/-), concentrations of superoxide anions and hydroxyl radical activity were measured in the extracellular space by microdialysis. A 15-min protocol of 180 isometric contractions induced a rapid, equivalent increase in reduction of cytochrome c as an index of superoxide anion concentrations in the extracellular space of Sod2+/+ and Sod2+/- mice, whereas hydroxyl radical activity measured by formation of 2,3-dihydroxybenzoate from salicylate increased only in the extracellular space of muscles of Sod2+/+ mice. The lack of a difference in increase in superoxide anion concentration in the extracellular space of Sod2+/+ and Sod2+/- mice after the contraction protocol supported the hypothesis that superoxide anions were not directly derived from mitochondria. In contrast, the data obtained suggest that the increase in hydroxyl radical concentration in the extracellular space of muscles from wild-type mice after the contraction protocol most likely results from degradation of hydrogen peroxide generated by MnSOD activity.

Reactive Oxygen Species Influence Biofilm Formation of Acidophilic Mineral-Oxidizing Bacteria on Pyrite

2015 ◽  
Vol 1130 ◽  
pp. 118-122 ◽  
Author(s):  
Sören Bellenberg ◽  
Dieu Huynh ◽  
Laura Castro ◽  
Maria Boretska ◽  
Wolfgang Sand ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Hydroxyl Radicals ◽  
Biofilm Formation ◽  
Laser Scanning ◽  
Reactive Oxygen ◽  
Laser Scanning Microscopy ◽  
Metal Sulfides ◽  
Confocal Laser ◽  
Water Molecules ◽  
Oxygen Species

Reactive oxygen species (ROS), such as hydrogen peroxide (H2O2), superoxide (O2-) and hydroxyl radicals (OH.) are known to be formed on the surface of metal sulfides in aqueous solution under oxic and anoxic conditions. Consequently bacteria which have not been adapted to their presence are metabolically inhibited [1], presumably due to the presence of these ROS. Pyrite-grown cells ofAcidithiobacillus ferrooxidansT, in contrast to iron (II)-grown cells, were able to oxidize iron (II)-ions or pyrite after 24 h starvation and contact with 1 mM externally added H2O2. In this study, similar results were obtained withAcidiferrobactersp. SPIII/3. However,Acidithiobacillus ferrivoransSS3 showed the highest tolerance towards contact with H2O2, whileLeptospirillum ferrooxidansDSM 2391 was most sensitive. Similar results were obtained after exposure to defined doses of gamma radiation, which cleaves water molecules and generates ROS. In this study members of the three aforementioned genera of mineral-oxidizing bacteria were compared regarding their ability to survive, colonize pyrite and to oxidize iron (II)-ions after exposure to different concentrations of H2O2. Pyrite colonization was studied after exposure to endogenous ROS formed on pyrite or after external addition of H2O2using confocal laser scanning microscopy (CLSM).

scholarly journals Reactive Oxygen Species and Induction of Lignin Peroxidase in Phanerochaete chrysosporium

2003 ◽  
Vol 69 (11) ◽  
pp. 6500-6506 ◽  
Author(s):  
Paula A. Belinky ◽  
Nufar Flikshtein ◽  
Sergey Lechenko ◽  
Shimon Gepstein ◽  
Carlos G. Dosoretz
Keyword(s):  
Reactive Oxygen Species ◽  
Oxidative Damage ◽  
Phanerochaete Chrysosporium ◽  
Hydroxyl Radicals ◽  
Lignin Peroxidase ◽  
Manganese Superoxide Dismutase ◽  
Reactive Oxygen ◽  
Radical Scavenger ◽  
Oxygen Species ◽  
Atmospheric Air

ABSTRACT We studied oxidative stress in lignin peroxidase (LIP)-producing cultures (cultures flushed with pure O2) of Phanerochaete chrysosporium by comparing levels of reactive oxygen species (ROS), cumulative oxidative damage, and antioxidant enzymes with those found in non-LIP-producing cultures (cultures grown with free exchange of atmospheric air [control cultures]). A significant increase in the intracellular peroxide concentration and the degree of oxidative damage to macromolecules, e.g., DNA, lipids, and proteins, was observed when the fungus was exposed to pure O2 gas. The specific activities of manganese superoxide dismutase, catalase, glutathione reductase, and glutathione peroxidase and the consumption of glutathione were all higher in cultures exposed to pure O2 (oxygenated cultures) than in cultures grown with atmospheric air. Significantly higher gene expression of the LIP-H2 isozyme occurred in the oxygenated cultures. A hydroxyl radical scavenger, dimethyl sulfoxide (50 mM), added to the culture every 12 h, completely abolished LIP expression at the mRNA and protein levels. This effect was confirmed by in situ generation of hydroxyl radicals via the Fenton reaction, which significantly enhanced LIP expression. The level of intracellular cyclic AMP (cAMP) was correlated with the starvation conditions regardless of the oxygenation regimen applied, and similar cAMP levels were obtained at high O2 concentrations and in cultures grown with atmospheric air. These results suggest that even though cAMP is a prerequisite for LIP expression, high levels of ROS, preferentially hydroxyl radicals, are required to trigger LIP synthesis. Thus, the induction of LIP expression by O2 is at least partially mediated by the intracellular ROS.

scholarly journals The oxidation of dehydroascorbic acid and 2,3-diketogulonate by distinct reactive oxygen species

2018 ◽  
Vol 475 (21) ◽  
pp. 3451-3470 ◽  
Author(s):  
Rebecca A. Dewhirst ◽  
Stephen C. Fry
Keyword(s):  
Reactive Oxygen Species ◽  
Ascorbic Acid ◽  
Hydroxyl Radicals ◽  
Reactive Oxygen ◽  
Dehydroascorbic Acid ◽  
Oxidation Products ◽  
Oxygen Species ◽  
H2o2 Treatment ◽  
Fold Excess

l-Ascorbate, dehydro-l-ascorbic acid (DHA), and 2,3-diketo-l-gulonate (DKG) can all quench reactive oxygen species (ROS) in plants and animals. The vitamin C oxidation products thereby formed are investigated here. DHA and DKG were incubated aerobically at pH 4.7 with peroxide (H2O2), ‘superoxide’ (a ∼50 : 50 mixture of and ), hydroxyl radicals (•OH, formed in Fenton mixtures), and illuminated riboflavin (generating singlet oxygen, 1O2). Products were monitored electrophoretically. DHA quenched H2O2 far more effectively than superoxide, but the main products in both cases were 4-O-oxalyl-l-threonate (4-OxT) and smaller amounts of 3-OxT and OxA + threonate. H2O2, but not superoxide, also yielded cyclic-OxT. Dilute Fenton mixture almost completely oxidised a 50-fold excess of DHA, indicating that it generated oxidant(s) greatly exceeding the theoretical •OH yield; it yielded oxalate, threonate, and OxT. 1O2 had no effect on DHA. DKG was oxidatively decarboxylated by H2O2, Fenton mixture, and 1O2, forming a newly characterised product, 2-oxo-l-threo-pentonate (OTP; ‘2-keto-l-xylonate’). Superoxide yielded negligible OTP. Prolonged H2O2 treatment oxidatively decarboxylated OTP to threonate. Oxidation of DKG by H2O2, Fenton mixture, or 1O2 also gave traces of 4-OxT but no detectable 3-OxT or cyclic-OxT. In conclusion, DHA and DKG yield different oxidation products when attacked by different ROS. DHA is more readily oxidised by H2O2 and superoxide; DKG more readily by 1O2. The diverse products are potential signals, enabling organisms to respond appropriately to diverse stresses. Also, the reaction-product ‘fingerprints’ are analytically useful, indicating which ROS are acting in vivo.

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