A study on acute toxicity and solvent capacity of solvate ionic liquids in vivo using a zebrafish model (Danio rerio)

2016 ◽  
Vol 40 (8) ◽  
pp. 6599-6603 ◽  
Author(s):  
Prusothman Yoganantharajah ◽  
Daniel J. Eyckens ◽  
Jessie L. Pedrina ◽  
Luke C. Henderson ◽  
Yann Gibert
Keyword(s):  
Ionic Liquids ◽  
Acute Toxicity ◽  
Danio Rerio ◽  
Zebrafish Model

The in vivo toxicity of several solvate ionic liquids have been assessed using a zebrafish model.

Acute Toxicity of Keladi Tikus (Typhonium flagelliforme (Lodd.) Blume) Ethanol Extract on Zebrafish (Danio rerio) Embryo In Vivo

2020 ◽  
Author(s):  
Fajar Fakri ◽  
Loly Subhiaty Idrus ◽  
Maria Alexandra Iskandar ◽  
Indra Wibowo ◽  
I Ketut Adnyana
Keyword(s):  
Acute Toxicity ◽  
Danio Rerio ◽  
Toxicity Testing ◽  
Ethanol Extract ◽  
Embryo Morphology ◽  
Zebrafish Embryos ◽  
Zebrafish Model ◽  
Pericardial Edema ◽  
High Concentrations

Keladi tikus (Typhonium flagelliforme (Lodd.) Blume) is an Indonesian medicinal plant that has various pharmacological properties. Zebrafish (Danio rerio) has been proposed as a model that can bridge the gap between cell assays and rodent assays. Evaluation of the toxic effects of natural products using the Zebrafish model can be assessed starting from the blastula stage of embryonic development. This study aims to investigate the potential acute toxicity effect of keladi tikus-ethanol extract (KTEE) using zebrafish embryos. A static non-replacement regime for acute toxicity testing was used. Wild-type zebrafish embryos were exposed to various concentrations of KTEE (50, 100, 200, 400, 800, 1600 µg/mL) starting at 6 hours post-fertilization (hpf) until 96 hpf. The results showed that the survival rate of zebrafish embryos decreased as the concentration of the test extract increased. The LC50 values of KTEE were 494.553 µg/mL at 96 hpf and 555.787 µg/mL at 72 hpf. Embryotoxicity effect of KTEE includes hatching delays and decreased heartrate on zebrafish embryos, especially at high concentrations. KTEE also caused abnormalities in embryo morphology, including pericardial edema, jaw and tail deformity.

Acute toxicity of ionic liquids for three freshwater organisms: Pseudokirchneriella subcapitata, Daphnia magna and Danio rerio

2009 ◽  
Vol 72 (4) ◽  
pp. 1170-1176 ◽  
Author(s):  
Carlo Pretti ◽  
Cinzia Chiappe ◽  
Ilaria Baldetti ◽  
Sara Brunini ◽  
Gianfranca Monni ◽  
...  
Keyword(s):  
Ionic Liquids ◽  
Acute Toxicity ◽  
Daphnia Magna ◽  
Danio Rerio ◽  
Pseudokirchneriella Subcapitata ◽  
Freshwater Organisms

scholarly journals Acute Toxicity of Cigarette from Vietnam on in vivo Model Zebrafish (Danio rerio) Larvae

2021 ◽  
Vol 37 (4) ◽  
Author(s):  
Kieu Kien Trung ◽  
Nguyen Thi Huyen Trang ◽  
Vu Thi Hien ◽  
Nguyen Lai Thanh
Keyword(s):  
Acute Toxicity ◽  
Danio Rerio ◽  
Classification Criteria ◽  
In Vivo Model ◽  
Health Issues ◽  
Tobacco Products ◽  
Zebrafish Larvae ◽  
Particle Matter ◽  
Total Particle

Smoking is widely known to has a major contributor to public health issues both in worldwide and Vietnam. At the same time, Vietnam have a large number of smokers and the market is filled with diverse brand of tobacco products. In this study, we sampled 6 types of cigarette: ThangLong, Vina, Craven, Demi, Maxx and Bastos from local stores and conducted toxicological test on zebrafish larvae. Our results show that there are varied in toxicological properties of total particle matter collected from different cigarette brands in lethal and morphology effects on zebrafish embryos. LC50 of 6 brands after 96 hours of exposure were: ThangLong = 48.7 mg/L, Vina = 45.96 mg/L, Craven = 80.52 mg/L, Demi = 30.91 mg/L, Maxx = 83.54 mg/L and Bastos = 74.92 mg/L. They are lower than 100 mg/L, which put them under Category 2 and 3 in the GHS classification criteria for acute toxicity.

scholarly journals Deep Learning Approach for Quantification of Fluorescently Labeled Blood Cells in Danio rerio (Zebrafish)

2021 ◽  
Vol 15 ◽  
pp. 117793222110377
Author(s):  
Samrat Thapa ◽  
David L Stachura
Keyword(s):  
Deep Learning ◽  
Danio Rerio ◽  
Blood Cells ◽  
Learning Algorithm ◽  
Model Systems ◽  
Zebrafish Model ◽  
High Throughput Analysis ◽  
Manual Counting ◽  
Programming Knowledge

Neutrophils are a type of white blood cell essential for the function of the innate immune system. To elucidate mechanisms of neutrophil biology, many studies are performed in vertebrate animal model systems. In Danio rerio (zebrafish), in vivo imaging of neutrophils is possible due to transgenic strains that possess fluorescently labeled leukocytes. However, due to the relative abundance of neutrophils, the counting process is laborious and subjective. In this article, we propose the use of a custom trained “you only look once” (YOLO) machine learning algorithm to automate the identification and counting of fluorescently labeled neutrophils in zebrafish. Using this model, we found the correlation coefficient between human counting and the model equals r = 0.8207 with an 8.65% percent error, while variation among human counters was 5% to 12%. Importantly, the model was able to correctly validate results of a previously published article that quantitated neutrophils manually. While the accuracy can be further improved, this model notably achieves these results in mere minutes compared with hours via standard manual counting protocols and can be performed by anyone with basic programming knowledge. It further supports the use of deep learning models for high throughput analysis of fluorescently labeled blood cells in the zebrafish model system.

scholarly journals Effects of Zinc Ions Released from Ti-NW-Zn Surface on Osteogenesis and Angiogenesis in Vitro and in an in Vivo Zebrafish Model

2021 ◽  
Author(s):  
Wen-qing Zhu ◽  
Kang Li ◽  
Shan Su ◽  
Wei Chen ◽  
Yao Liu ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Signaling Pathway ◽  
Danio Rerio ◽  
Erk Signaling ◽  
Zinc Ions ◽  
Adult Fish ◽  
Zebrafish Model ◽  
Positive Effects

Abstract Objective: Zinc-modified titanium materials have been widely applied in oral implants, among them, our previous studies have also successfully prepared a novel acid-etched micro-structured titanium surface modified with zinc-containing nanowires (Ti-NW-Zn). However, there are very few reports concerned about the biological safeties of zinc ions released from material surface and the appropriate concentration range of released zinc ions which was more conducive to angiogenesis and bone regeneration. Danio rerio (Zebrafish) represents a powerful alternative in vivo model to study both angiogenesis and osteogenesis. Then, the aim of this study is to investigate the effects of zinc ions released from Ti-NW-Zn surfaces on angiogenesis and osteogenesis via zebrafish model, and further revealed the relationship between angiogenesis and osteogenesis via HUVECs and MC3T3-E1s in vitro models.Materials and methods: Tg (Fli‐1:EGFP)y1 zebrafish embryos were used to investigate the in vivo angiogenesis exposure to zinc ions, Wild-type Zebrafish (Danio rerio) adult fish and their embryos were used to investigate the in vivo bone regeneration and survival rate. Cell experiments including proliferation, adhesion, osteogenic differentiation and signaling pathway experiments were carried out by CCK-8, Laser scanning confocal microscope and Western blot, etc. Zinc ion release assay was detected by a Zinc assay kit.Results: The zinc ions released from Ti-NW-Zn surfaces were far lower than median lethal concentrations (LCs) of both embryos and adult fish, which was beneficial to osteogenesis and angiogenesis promotion of zebrafish model. Moreover, the appropriate concentration range of zinc ions was 1-2 ppm in vitro models, which could induce HUVECs proliferations, and the conditioned medium (CM) collected from the supernatant of 1-2 ppm zinc ions-induced HUVECs medium could obviously promote MC3T3-E1 osteoblasts behaviors via activated the MAPK/ERK signaling pathway.Conclusion: Zinc ions released from Ti-NW-Zn surfaces was at a biological safe and appropriate concentration, which could promote the angiogenesis and osteogenesis in vivo and vitro. The positive effects of the appropriate concentration of zinc ions on osteoblast behaviors might be regulated by activating the MAPK/ERK signaling pathway.

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