Lead (Pb) exposure reduces global DNA methylation level by non-competitive inhibition and alteration of dnmt expression

Metallomics ◽  
2017 ◽  
Vol 9 (2) ◽  
pp. 149-160 ◽  
Author(s):  
Oscar F. Sanchez ◽  
Jinyoung Lee ◽  
Nathaphon Yu King Hing ◽  
Seong-Eun Kim ◽  
Jennifer L. Freeman ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Methylation Level ◽  
Competitive Inhibition ◽  
Global Dna Methylation ◽  
Pb Exposure

scholarly journals Repeats as global DNA methylation marker in bovine preimplantation embryos

2017 ◽  
Vol 62 (No. 2) ◽  
pp. 43-50 ◽  
Author(s):  
W. Li ◽  
A. Van Soom ◽  
L. Peelman
Keyword(s):  
Dna Methylation ◽  
Mutation Rate ◽  
Methylation Level ◽  
Methylation Status ◽  
Repetitive Sequences ◽  
Cell Types ◽  
Blastocyst Stage ◽  
Repeat Sequence ◽  
Preimplantation Embryos ◽  
Global Dna Methylation

DNA methylation undergoes dynamic changes and is a crucial part of the epigenetic regulation during mammalian early development. To determine the DNA methylation levels in bovine embryos, we applied a bisulfite sequencing based method aimed at repetitive sequences including three retrotransposons (L1_BT, BovB, and ERV1-1-I_BT) and Satellite I. A more accurate estimate of the global DNA methylation level compared to previous methods using only one repeat sequence, like Alu, could be made by calculation of the weighted arithmetic mean of multiple repetitive sequences, considering the copy number of each repetitive sequence. Satellite I and L1_BT showed significant methylation reduction at the blastocyst stage, while BovB and ERV1-1-I_BT showed no difference. The mean methylation level of the repetitive sequences during preimplantation development was the lowest at the blastocyst stage. No methylation difference was found between embryos cultured in 5% and 20% O<sub>2</sub>. Because mutations of CpGs negatively influence the calculation accuracy, we checked the mutation rate of the sequenced CpG sites. Satellite I and L1_BT showed a relatively low mutation rate (1.92 and 3.72% respectively) while that of ERV1-1-I_BT and BovB was higher (11.95 and 24% respectively). Therefore we suggest using a combination of repeats with low mutation rate, taking into account the proportion of each sequence, as a relatively quick marker for the global DNA methylation status of preimplantation stages and possibly also for other cell types.

scholarly journals Sperm global DNA methylation level: association with semen parameters and genome integrity

Andrology ◽  
2015 ◽  
Vol 3 (2) ◽  
pp. 235-240 ◽  
Author(s):  
D. Montjean ◽  
A. Zini ◽  
C. Ravel ◽  
S. Belloc ◽  
A. Dalleac ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Methylation Level ◽  
Semen Parameters ◽  
Genome Integrity ◽  
Global Dna Methylation

scholarly journals Influence of maternal age on intracytoplasmic sperm injection outcome and global deoxyribonucleic acid methylation in women undergoing intracytoplasmic sperm injection cycle

2021 ◽  
Vol 10 (6) ◽  
pp. 2185
Author(s):  
Mohammed M. Laqqan ◽  
Maged M. Yassin
Keyword(s):  
Dna Methylation ◽  
Intracytoplasmic Sperm Injection ◽  
Methylation Level ◽  
Maternal Age ◽  
Deoxyribonucleic Acid ◽  
Global Dna Methylation ◽  
Age Group ◽  
Icsi Cycle ◽  
Injection Cycle ◽  
Positive Significant Correlation

Background: Intracytoplasmic sperm injection needs sufficient oocytes of high quality in order to increase the rate of fertilization and pregnancy. This study was designed to investigate the influence of maternal age on the ICSI outcomes in women undergoing to first ICSI cycle and to evaluate the influence of maternal age on global DNA methylation.Methods: A total of 242 females were included in this study with a mean age of 30.5±7.3 years. The participants were divided into three groups depending on women's age≤25, N=70; 26-35, N=102 and>35, N=70). The genomic DNA was isolated from the blood samples, then the global DNA methylation was evaluated using ELISA.Results: A significant reduction has been found in the level of anti-Müllerian hormone (AMH), total number of the collected oocyte, mature oocytes, fertilized oocytes and number of embryos transferred in the older females compared to the younger group (p<0.001). While a significant increase has been found in global DNA methylation level in the older females compared to the younger group (p<0.001). A positive significant correlation has been found between global DNA methylation level and maternal age (p<0.001). In contrast, a negative significant correlation has been shown between AMH level, mature oocytes and maternal age (p<0.001).Conclusions: Maternal age has a significant influence on the number of mature oocytes, number of embryos transferred and global DNA methylation. The pregnancy chance is more in the age group less than 35 years.

Global DNA Methylation Level in Whole Blood as a Biomarker in Head and Neck Squamous Cell Carcinoma

2007 ◽  
Vol 16 (1) ◽  
pp. 108-114 ◽  
Author(s):  
Debra Ting Hsiung ◽  
Carmen J. Marsit ◽  
E. Andres Houseman ◽  
Karen Eddy ◽  
C. Sloane Furniss ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Whole Blood ◽  
Methylation Level ◽  
Neck Squamous Cell Carcinoma ◽  
Global Dna Methylation

scholarly journals Application of the LUminometric Methylatoion Assay for plant ecological researches: the study of global DNA methylation in leaves of Elodea canadensis under laboratory conditions and in leaves of fen orchid from wild populations

Plant Omics ◽  
2020 ◽  
pp. 30-36
Author(s):  
Natalja Škute ◽  
Marina Savicka ◽  
Aleksandrs Petjukevičs ◽  
Nadežda Harlamova
Keyword(s):  
Dna Methylation ◽  
Environmental Factors ◽  
Methylation Level ◽  
Aquatic Macrophyte ◽  
Elodea Canadensis ◽  
Global Dna Methylation ◽  
Dna Amount ◽  
Methylation Assay ◽  
Liparis Loeselii ◽  
Plant Ecological

The epigenetic changes in the genome of plants are one of the important regulatory mechanisms in response to the environmental factors. The LUminometric Methylation Assay (LUMA) requires a relatively small DNA amount, a short processing time and is easily adapted for species with a non-resolved genome. The LUMA has not been previously used for ecological research of plants. In this research, LUMA was used for the first time to investigate the changes of global DNA methylation under different environmental factors in the leaves of different plants. The influence of salinity on global DNA methylation was studied on aquatic macrophyte Elodea canadensis Michx, which grew in aquatic tanks under different NaCl concentrations. After the third week of growth, the HpaII/MspI ratio was measured by LUMA and global DNA methylation percentages were calculated. The results showed salt stress-induced changes in the global DNA methylation level in E.canadensis leaves, compared to control. The response was salt dose-dependent. The changes of global DNA methylation in wildlife plant populations were analogically assessed on fen orchid Liparis loeselii (L.) Rich. It was shown that global DNA methylation level was higher in leaves of these plants in Engure Lake, where there are temporary changes in water regime, compared to leaves of plants from other places. It was assumed that global GC-DNA methylation plays an essential role in the survival of this plant. Therefore, we show the possibilities of using the LUMA method for epigenetic study of different plants ecological researches.

scholarly journals Global DNA Methylation Of Brain Neurons In Acute Poisoning With Clozapine And Its Combination With Alcohol: An Experimental Study

2021 ◽  
Vol 10 (3) ◽  
Author(s):  
Anastasiya S. Babkina ◽  
Maryam B. Khadzhiyeva ◽  
Irina V. Ostrova ◽  
Ivan A. Ryzhkov ◽  
Arkady M. Golubev
Keyword(s):  
Dna Methylation ◽  
Dna Fragmentation ◽  
Methylation Level ◽  
Gastric Tube ◽  
Acute Poisoning ◽  
Control Group ◽  
Global Dna Methylation ◽  
Brain Neurons ◽  
Ethanol Group ◽  
Risk Of Death

Background — Acute poisoning with atypical neuroleptic clozapine is characterized by rapid progression, high risk of death and severe neurological manifestations. Neurotoxic effects of this pharmaceutical drug have also been reported at therapeutic doses. The pathogenesis of brain damage in acute clozapine poisoning is not fully understood. Changes in DNA methylation level may play an important role in the mechanisms of drug neurotoxicity. The available data on the effect of clozapine on brain cell DNA provide a rationale for studying the epigenetic aspects of the pathogenesis of acute poisoning with this neuroleptic agent. The objective of our study was to evaluate the global DNA methylation level in rat brain neurons in acute poisoning with clozapine and its combination with ethanol. Material and methods — Clozapine – 150 mg/kg in 2.0 ml of normal saline solution, or clozapine – 150 mg/kg in 2.0 ml of 40% ethanol were administered via a gastric tube to adult male Wistar rats (n=21) under anesthesia with sevoflurane. In the control group, saline was administered via a gastric tube. Animals were euthanized four hours after drug administration. Autopsy was performed with the collection of brain samples for histochemical examination and determination of the DNA methylation level using the fluorometric method. To detect DNA in sections of paraffin-embedded tissue, we used the Feulgen staining. The TUNEL method was employed to detect DNA fragmentation. Results — An increase in the level of global DNA methylation in brain neurons was found in the clozapine and clozapine+ethanol groups. The average level of methylated DNA in the clozapine+ethanol group was higher than in the control group or clozapine group (2.56±0.31 vs. 1.35±0.1, p=0.007 and 1.70±0.33, p=0.044, respectively). An increase in the mean optical density of the cortical neuron nuclei was observed in the clozapine+ethanol group compared with the control group and clozapine group. DNA fragmentation was not detected in any experimental group. Conclusion — Acute poisoning with clozapine in combination with alcohol caused an increase in the global DNA methylation level in brain neurons, which may have played a significant role in the pathogenesis of acute clozapine poisoning and could be an important factor in the neurotoxicity of this medication.

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