scholarly journals Improved bovine embryo production in an oviduct-on-a-chip system: prevention of poly-spermic fertilization and parthenogenic activation

Lab on a Chip ◽  
2017 ◽  
Vol 17 (5) ◽  
pp. 905-916 ◽  
Author(s):  
Marcia A. M. M. Ferraz ◽  
Heiko H. W. Henning ◽  
Pedro F. Costa ◽  
Jos Malda ◽  
Ferry P. Melchels ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
3D Printing ◽  
Cell Cultures ◽  
Bovine Embryo ◽  
Embryo Production ◽  
Epithelial Cell Cultures ◽  
Oviduct Epithelial Cell

The use of 3D-printing in bovine oviduct epithelial cell cultures allows better bio-mimicking of embryo production than classical in vitro fertilization.

scholarly journals Culturing the Unculturable: Human Coronavirus HKU1 Infects, Replicates, and Produces Progeny Virions in Human Ciliated Airway Epithelial Cell Cultures

2010 ◽  
Vol 84 (21) ◽  
pp. 11255-11263 ◽  
Author(s):  
Krzysztof Pyrc ◽  
Amy C. Sims ◽  
Ronald Dijkman ◽  
Maarten Jebbink ◽  
Casey Long ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Cultures ◽  
Airway Epithelium ◽  
Airway Epithelial Cell ◽  
Clinical Sample ◽  
Airway Epithelial ◽  
Human Coronavirus ◽  
Epithelial Cell Cultures

ABSTRACT Culturing newly identified human lung pathogens from clinical sample isolates can represent a daunting task, with problems ranging from low levels of pathogens to the presence of growth suppressive factors in the specimens, compounded by the lack of a suitable tissue culture system. However, it is critical to develop suitable in vitro platforms to isolate and characterize the replication kinetics and pathogenesis of recently identified human pathogens. HCoV-HKU1, a human coronavirus identified in a clinical sample from a patient with severe pneumonia, has been a major challenge for successful propagation on all immortalized cells tested to date. To determine if HCoV-HKU1 could replicate in in vitro models of human ciliated airway epithelial cell cultures (HAE) that recapitulate the morphology, biochemistry, and physiology of the human airway epithelium, the apical surfaces of HAE were inoculated with a clinical sample of HCoV-HKU1 (Cean1 strain). High virus yields were found for several days postinoculation and electron micrograph, Northern blot, and immunofluorescence data confirmed that HCoV-HKU1 replicated efficiently within ciliated cells, demonstrating that this cell type is infected by all human coronaviruses identified to date. Antiserum directed against human leukocyte antigen C (HLA-C) failed to attenuate HCoV-HKU1 infection and replication in HAE, suggesting that HLA-C is not required for HCoV-HKU1 infection of the human ciliated airway epithelium. We propose that the HAE model provides a ready platform for molecular studies and characterization of HCoV-HKU1 and in general serves as a robust technology for the recovery, amplification, adaptation, and characterization of novel coronaviruses and other respiratory viruses from clinical material.

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