A centrifugal microfluidic device with integrated gold leaf electrodes for the electrophoretic separation of DNA

Lab on a Chip ◽  
2016 ◽  
Vol 16 (23) ◽  
pp. 4569-4580 ◽  
Author(s):  
Brandon L. Thompson ◽  
Christopher Birch ◽  
Daniel A. Nelson ◽  
Jingyi Li ◽  
Jacquelyn A. DuVall ◽  
...  
Keyword(s):  
High Resolution ◽  
Microfluidic Device ◽  
Dna Fragments ◽  
Electrophoretic Separation ◽  
Gold Leaf

A centrifugal microdevice with integrated gold leaf electrodes capable of high resolution electrophoretic separation of DNA fragments.

scholarly journals High-resolution liquid chromatography of DNA fragments on non-porous poly(styrene-divinylbenzene) particles

1993 ◽  
Vol 21 (5) ◽  
pp. 1061-1066 ◽  
Author(s):  
Christian G. Huber ◽  
Peter J. Oefner ◽  
Eugen Preuss ◽  
Günther K. Bonn
Keyword(s):  
Liquid Chromatography ◽  
High Resolution ◽  
Dna Fragments ◽  
Styrene Divinylbenzene ◽  
Porous Poly

A Multi-Functional Microfluidic Device Compatible with Widefield and Light Sheet Imaging

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Regan P Moore ◽  
Ellen C O’Shaughnessy ◽  
Yu Shi ◽  
Ana T Nogueira ◽  
Katelyn M Heath ◽  
...  
Keyword(s):  
High Resolution ◽  
Microfluidic Device ◽  
Super Resolution ◽  
Light Sheet ◽  
Ethylene Propylene ◽  
Super Resolution Microscopy ◽  
Fluorinated Ethylene Propylene

We present a microfluidic device compatible with high resolution light sheet and super-resolution microscopy. Our device is a 150 μm thick chamber with a transparent fluorinated ethylene propylene (FEP) cover...

Rapid Separation and Manipulation of DNA by a Ratcheting Electrophoresis Microchip (REM)

2002 ◽  
Author(s):  
Katherine Dunphy ◽  
Veljko Milanovic ◽  
Samantha Andrews ◽  
Taku Ohara ◽  
Arun Majumdar
Keyword(s):  
Silicon Nitride ◽  
Microfluidic Device ◽  
Platinum Electrodes ◽  
Electrophoretic Separation ◽  
Rapid Separation ◽  
Initial Testing ◽  
Electrophoresis Microchip ◽  
Parallel Arrays ◽  
Short Times ◽  
Partial Success

The Ratcheting Electrophoresis Microchip (REM) is a microfluidic device for electrophoretic separation of biomolecules such as DNA and proteins. By using thousands of electrodes along the length of a microchannel, the REM separates molecules using low applied voltages (∼1 V) in short times (< 1 minute). This paper describes the microfabriation of the REM and initial testing results. Parallel arrays of platinum electrodes are fabricated on a silicon chip with a pitch of 10 μm. Two types of channels are fabricated: silicon nitride channels fabricated on the chip and poly(dimelthylsiloxane) (PDMS) channels fabricated separately and attached to the chip. Initial testing shows partial success with the PDMS channels and promis ing results for the silicon nitride channels.

The Electrophoretic Separation of Curved Cisplatin-Modifled DNA Fragments on Polyacrylamide Gels Is Dependent on the Voltage Gradient

1998 ◽  
Vol 53 (9-10) ◽  
pp. 921-923
Author(s):  
Julia Yaneva ◽  
Jordanka Zlatanova
Keyword(s):  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Threshold Level ◽  
Voltage Gradient ◽  
Dna Fragments ◽  
Electrophoretic Separation ◽  
Polyacrylamide Gels ◽  
Curved Dna ◽  
Electrophoretic Behavior ◽  
Param Eters

Polyacrylamide gel electrophoresis has been widely used to study DNA fragments containing sequence-dependent curvature. The anomalous electrophoretic behavior of curved DNA fragments on such gels allows their separation from straight fragments of the same length. Here we demonstrate that polyacrylamide gels can be successfully used to resolve DNA fragments modified at a single site by the antitumor drug cis-diamminedichloroplatinum(II) (cis-DDP, cisplatin) from their unmodified counterparts. However, the resolution strongly depends on the voltage gradient, being completely lost when it drops below a certain threshold level. The param eters of the electric field do not affect separation of ‘normal’ DNA fragments of comparable length.

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