Optofluidic restricted imaging, spectroscopy and counting of nanoparticles by evanescent wave using immiscible liquids

Lab on a Chip ◽  
2016 ◽  
Vol 16 (16) ◽  
pp. 3007-3014 ◽  
Author(s):  
L. Liang ◽  
Y. F. Zuo ◽  
W. Wu ◽  
X. Q. Zhu ◽  
Y. Yang
Keyword(s):  
Evanescent Wave ◽  
Total Internal Reflection ◽  
Imaging Spectroscopy ◽  
Internal Reflection ◽  
Immiscible Liquids ◽  
Immiscible Flows ◽  
Optofluidic Chip

An integrated optofluidic chip for restricted imaging, spectroscopy and counting of nanoparticles using the evanescent wave of total internal reflection at the interface of immiscible flows.

Tracking of Nanoparticles Using Evanescent Wave Microscopy

Volume 4 ◽  
2004 ◽  
Author(s):  
Arindam Banerjee ◽  
Kenneth D. Kihm
Keyword(s):  
Diffusion Coefficient ◽  
Evanescent Wave ◽  
Total Internal Reflection ◽  
Tracer Particle ◽  
Three Dimensional ◽  
Internal Reflection ◽  
Brownian Diffusion ◽  
Wall Region ◽  
Identification Algorithm ◽  
Polystyrene Spheres

A novel three-dimensional tracking technique of nanoparticles in nanometer spatial resolution using a Ratiometric Total Internal Reflection Fluorescence Microscopy (R-TIRFM) is presented. Evanescent waves from the total internal reflection of an argon-ion laser (488 nm) are used to generate a thin sliced illumination field with its effective visualization range of 544-nm, equivalent to twice of the penetration depth of the evanescent wave field. Fluorescence-coated polystyrene spheres of 500-nm diameter (SG = 1.05) are used as tracers subjected to the Brownian diffusive motions. A ratiometric analysis of the fluorescence particle images together with a neural network particle-pair identification algorithm is used to track the tracer particle locations across multiple image frames in full three-dimensional ways. The techniques are used to examine the Brownian diffusive motion of nanoparticles as they approach the very near-wall region within a few hundred nanometers of a glass-water interface. The experimental results show that the measured Brownian diffusion coefficient is in good agreement with the theoretical hindered diffusion coefficient near a wall.

Two-Photon Excitation by the Evanescent Wave from Total Internal Reflection

1997 ◽  
Vol 247 (1) ◽  
pp. 69-76 ◽  
Author(s):  
Ignacy Gryczynski ◽  
Zygmunt Gryczynski ◽  
Joseph R. Lakowicz
Keyword(s):  
Evanescent Wave ◽  
Total Internal Reflection ◽  
Internal Reflection ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation
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