Chemical characterization of the glycated myofibrillar proteins from grass carp (Ctenopharyngodon idella) and their impacts on the human gut microbiota in vitro fermentation

2017 ◽  
Vol 8 (3) ◽  
pp. 1184-1194 ◽  
Author(s):  
Kaining Han ◽  
Ye Yao ◽  
Shiyuan Dong ◽  
Sun Jin ◽  
Hang Xiao ◽  
...  
Keyword(s):  
Grass Carp ◽  
Chemical Characterization ◽  
Ctenopharyngodon Idella ◽  
Myofibrillar Proteins ◽  
Human Gut ◽  
In Vitro Fermentation ◽  
Microbial Community Structures ◽  
Grass Carp Ctenopharyngodon Idella

Glycation greatly increased the anti-digestibility of myofibrillar proteins derived from grass carp, and affected the production of SCFAs and the microbial community structures inin vitrofecal fermentation.

Identification and molecular characterization of peroxiredoxin 2 in grass carp (Ctenopharyngodon idella)

2019 ◽  
Vol 92 ◽  
pp. 570-582
Author(s):  
Denghui Zhu ◽  
Rong Huang ◽  
Cheng Yang ◽  
Peipei Fu ◽  
Liangming Chen ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Grass Carp ◽  
Ctenopharyngodon Idella ◽  
Peroxiredoxin 2 ◽  
Grass Carp Ctenopharyngodon Idella

scholarly journals Investigating the Role of BATF3 in Grass Carp (Ctenopharyngodon idella) Immune Modulation: A Fundamental Functional Analysis

2019 ◽  
Vol 20 (7) ◽  
pp. 1687 ◽  
Author(s):  
Denghui Zhu ◽  
Rong Huang ◽  
Peipei Fu ◽  
Liangming Chen ◽  
Lifei Luo ◽  
...  
Keyword(s):  
Grass Carp ◽  
Immune Modulation ◽  
Nuclear Translocation ◽  
Bimolecular Fluorescence Complementation ◽  
Ctenopharyngodon Idella ◽  
Poly I:C ◽  
Mrna Levels ◽  
Basic Leucine Zipper ◽  
Grass Carp Ctenopharyngodon Idella

Basic leucine zipper transcription factor ATF-like (BATF)-3, belonging to activator protein 1 (AP-1) superfamily transcription factors, is essential for homeostatic development of CD8α+ classical dendritic cells activating CD8 T-cell responses to intracellular pathogens. In this study, the characteristics and cDNA cloning of the CiBATF3 molecule were described in grass carp (Ctenopharyngodon idella). CiBATF3 had abundant expression in immune-related organizations, including liver, spleen and gill, and grass carp reovirus (GCRV) infection had significantly changed its expression level. After Ctenopharyngodon idella kidney (CIK) cells were challenged with pathogen-associated molecular patterns (PAMPs), polyinosinic:polycytidylic acid (poly(I:C)) stimulation induced higher mRNA levels of CiBATF3 than that of lipopolysaccharide (LPS). Subcellular localization showed that CiBATF3-GFP was entirely distributed throughout cells and nuclear translocation of CiBATF3 was found after poly(I:C) treatment. Additionally, the interaction between CiBATF3 and interleukin 10 (IL-10) was proven by bimolecular fluorescence complementation (BiFC) system. The small interfering RNA (siRNA)-mediated CiBATF3 silencing showed that the mRNA of CiBATF3 and its downstream genes were down-regulated in vitro and in vivo. CiBATF3 played a negative regulatory role in the transcriptional activities of AP-1 and NF-κB reporter gene. In summary, the results may provide valuable information on fundamental functional mechanisms of CiBATF3.

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