Anti-inflammatory activity of 3β-hydroxycholest-5-en-7-one isolated from Hippocampus trimaculatus leach via inhibiting iNOS, TNF-α, and 1L-1β of LPS induced RAW 264.7 macrophage cells

2017 ◽  
Vol 8 (2) ◽  
pp. 788-795 ◽  
Author(s):  
Jingna Wu ◽  
Zhiyu Liu ◽  
Jie Su ◽  
Nan Pan ◽  
Qiuling Song
Keyword(s):  
Herbal Medicine ◽  
Inflammatory Activity ◽  
Raw 264.7 ◽  
Macrophage Cells ◽  
Raw 264.7 Macrophage ◽  
Tnf Α ◽  
Raw 264.7 Macrophage Cells ◽  
Anti Inflammatory

Hippocampus trimaculatus leach has been widely used in beverage and herbal medicine fabrication.

Anti-Inflammatory Action of Indonesian Black Garlic (IBG) Ethanol Extracts in LPS-stimulated RAW 264.7 Macrophage Cells

2021 ◽  
pp. 201-208
Author(s):  
Laurentia Adinda Pratidina ◽  
Novita Wijayanti ◽  
Seong Gu Hwang
Keyword(s):  
Cell Counting ◽  
Raw 264.7 ◽  
Macrophage Cells ◽  
Raw 264.7 Macrophage ◽  
Tnf Α ◽  
Raw 264.7 Macrophage Cells ◽  
New Type ◽  
Anti Inflammatory ◽  
Ethanol Extracts ◽  
Inflammatory Action

Indonesian Black Garlic (IBG) ethanol extracts is a new type of fresh garlic product, produced at high temperature and humidity. IBG has active antioxidant activity. Thy study aimed to evaluate the anti-inflammatory actions of IBG in RAW 264.7 macrophage cells. The cells were cultures and treated with different concentrations of IBG (50 to 1000 mg/mL). The cell counting kit-8 assay showed that IBG was not toxic up to 400 mg/mL. IBG significantly decreased the expression of inflammatory mediators in LPS-stimulated RAW 264.7 macrophage cells. Futhermore, IBG is shown to inhibit pro-inflammatory cytokines, this is evident from the decreased expressions of iNOS, COX-2, IL-6, NF-kB, and TNF-α in mRNA expressions and also on protein level. The results suggest that IBG can be used to prevent or cure inflammation-related diseases.

scholarly journals Production Optimization, Structural Analysis, and Prebiotic- and Anti-Inflammatory Effects of Gluco-Oligosaccharides Produced by Leuconostoc lactis SBC001

2021 ◽  
Vol 9 (1) ◽  
pp. 200
Author(s):  
Minhui Kim ◽  
Jae-Kweon Jang ◽  
Young-Seo Park
Keyword(s):  
Nitric Oxide ◽  
Production Optimization ◽  
Inflammatory Activity ◽  
Raw 264.7 ◽  
Macrophage Cells ◽  
Raw 264.7 Macrophage ◽  
Yeast Strains ◽  
Raw 264.7 Macrophage Cells ◽  
Anti Inflammatory ◽  
Leuconostoc Lactis

Leuconostoc lactis SBC001, isolated from chive, produces glucansucrase and synthesizes oligosaccharides through its enzymatic activity. This study was conducted to optimize oligosaccharide production using response surface methodology, analyze the structure of purified oligosaccharides, and investigate the prebiotic effect on 24 bacterial and yeast strains and the anti-inflammatory activity using RAW 264.7 macrophage cells. The optimal conditions for oligosaccharide production were a culture temperature of 30 °C and sucrose and maltose concentrations of 9.6% and 7.4%, respectively. Based on 1H-NMR spectroscopic study, the oligosaccharides were identified as gluco-oligosaccharides that consisted of 23.63% α-1,4 glycosidic linkages and 76.37% α-1,6 glycosidic linkages with an average molecular weight of 1137 Da. The oligosaccharides promoted the growth of bacterial and yeast strains, including Lactobacillus plantarum, L. paracasei, L. johnsonii, Leuconostoc mesenteroides, L. rhamnosus, and Saccharomyces cerevisiae. When lipopolysaccharide-stimulated RAW 264.7 cells were treated with the oligosaccharides, the production of nitric oxide was decreased; the expression of inducible nitric oxide synthase, tumor necrosis factor-α, interleukin (IL)-1β, IL-6, and IL-10 was suppressed; and the nuclear factor-kappa B signaling pathway was inhibited. In conclusion, the gluco-oligosaccharides obtained from Leu. lactis SBC001 exhibited a prebiotic effect on six bacterial and yeast strains and anti-inflammatory activity in RAW 264.7 macrophage cells.

Anti-inflammatory Effects of Madecassic Acid via the Suppression of NF-κB Pathway in LPS-Induced RAW 264.7 Macrophage Cells

Planta Medica ◽  
2009 ◽  
Vol 76 (03) ◽  
pp. 251-257 ◽  
Author(s):  
Jong-Heon Won ◽  
Ji-Sun Shin ◽  
Hee-Juhn Park ◽  
Hyun-Ju Jung ◽  
Duck-Jae Koh ◽  
...  
Keyword(s):  
Raw 264.7 ◽  
Macrophage Cells ◽  
Raw 264.7 Macrophage ◽  
Raw 264.7 Macrophage Cells ◽  
Anti Inflammatory
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