A novel polysaccharide isolated from mulberry fruits (Murus alba L.) and its selenide derivative: structural characterization and biological activities

2016 ◽  
Vol 7 (6) ◽  
pp. 2886-2897 ◽  
Author(s):  
Chun Chen ◽  
Bin Zhang ◽  
Xiong Fu ◽  
Rui Hai Liu
Keyword(s):  
Structural Characterization ◽  
Extraction Method ◽  
Biological Activities ◽  
Hot Water ◽  
Water Extraction ◽  
Chromatographic Purification ◽  
Hot Water Extraction ◽  
Mulberry Fruits

A novel polysaccharide (MFP3P) was isolated from Murus alba L. through the hot water extraction method followed by chromatographic purification.

Optimization of Enzymatic Hydrolytic Extraction Conditions for High Yield Polysaccharide Manufacturing from Liquid Fermented Materials of Hypsizigus Marmoreus

2011 ◽  
Vol 145 ◽  
pp. 154-158
Author(s):  
Pei Sheng Yan ◽  
Li Ya Ma ◽  
Li Xin Cao
Keyword(s):  
Regression Model ◽  
Extraction Method ◽  
Biological Activities ◽  
Uniform Design ◽  
Hot Water ◽  
Water Extraction ◽  
Neutral Protease ◽  
High Yield ◽  
Hot Water Extraction ◽  
Hypsizigus Marmoreus

Polysaccharides extracted from fruiting body and mycelia of higher fungi have been found to show various biological activities. Traditionally, these polysaccharides are manufactured by the hot water extraction method. Low polysaccharide yield is the disadvantage of this method. This paper reports the optimal conditions to manufacture polysaccharide with high-yield from Hypsizigus marmoreus mycelia using enzymatic hydrolytic extraction method. Neutral protease was selected as the most cost efficient among six enzymes. The uniform design was further conducted to optimize the enzymatic hydrolytic extraction conditions, and a regression model was constructed to predict polysaccharide yield. The optimized procedures was that 2% (w/w) of neutral protease was added into hydrolytic solution and incubated for 4 h at 43°C, then extracted one time at 100°C for 1 h. Under optimized procedures, the measured polysaccharide yield was 15.73±0.15 (mg/g), which was very close to the predicted value of 16.65 (mg/g). The results validated the accuracy of the regression model and the optimized procedure. When following the optimized procedures, polysaccharide yield was raised 75.0% over that before optimization, and increased by 122.5% and 104.8% over hot-water extraction and microwave assisted extraction methods respectively. This was the first study to apply uniform design for optimizing high yield manufacture of mycelia polysaccharides by enzymatic hydrolytic extraction. We concluded that enzymatic hydrolytic extraction was a simple, high yield method to manufacture polysaccharides from mushroom mycelia and might be used widely in polysaccharide-derived functional foods production.

Structural characterization and immunomodulatory activity of a new heteropolysaccharide from Prunella vulgaris

2015 ◽  
Vol 6 (5) ◽  
pp. 1557-1567 ◽  
Author(s):  
Chao Li ◽  
Lijun You ◽  
Xiong Fu ◽  
Qiang Huang ◽  
Shujuan Yu ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Extraction Method ◽  
Hot Water ◽  
Water Extraction ◽  
Immunomodulatory Activity ◽  
Prunella Vulgaris ◽  
Hot Water Extraction

A new heteropolysaccharide, here called P1, was isolated from the fruit clusters of Prunella vulgaris using a hot water extraction method.

Structural characterization and immunomodulatory activity of a novel polysaccharide from Ficus carica

2018 ◽  
Vol 9 (7) ◽  
pp. 3930-3943 ◽  
Author(s):  
Juan Du ◽  
Jingjing Li ◽  
Jianhua Zhu ◽  
Chunhua Huang ◽  
Sixue Bi ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Structural Characterization ◽  
Hot Water ◽  
Water Extraction ◽  
Ficus Carica ◽  
Immunomodulatory Activity ◽  
Hot Water Extraction ◽  
Chromatographic Methods

A novel polysaccharide (FCPW80-2) with a molecular weight of 1.21 × 105 Da was first isolated from Ficus carica through hot water extraction and several chromatographic methods.

scholarly journals Pressurized Hot Water Extraction of Okra Seeds Reveals Antioxidant, Antidiabetic and Vasoprotective Activities

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1645
Author(s):  
Eng Shi Ong ◽  
Christina Liu Ying Oh ◽  
Joseph Choon Wee Tan ◽  
Su Yi Foo ◽  
Chen Huei Leo
Keyword(s):  
Biological Activities ◽  
Vascular Inflammation ◽  
Single Step ◽  
Polyphenolic Compounds ◽  
Hot Water ◽  
Water Extraction ◽  
Extraction Temperature ◽  
Hot Water Extraction ◽  
Pressurized Hot Water Extraction ◽  
Different Parts

Abelmoschus esculentus L. Moench (okra) is a commonly consumed vegetable that consists of the seeds and peel component which are rich in polyphenolic compounds. The aim of this study is to utilize pressurized hot water extraction (PHWE) for the extraction of bioactive phytochemicals from different parts of okra. A single step PHWE was performed at various temperatures (60 °C, 80 °C, 100 °C and 120 °C) to determine which extraction temperature exhibits the optimum phytochemical profile, antioxidant and antidiabetic activities. The optimum temperature for PHWE extraction was determined at 80 °C and the biological activities of the different parts of okra (Inner Skin, Outer Skin and Seeds) were characterized using antioxidant (DPPH and ABTS), α-glucosidase and vasoprotective assays. Using PHWE, the different parts of okra displayed distinct phytochemical profiles, which consist of primarily polyphenolic compounds. The okra Seeds were shown to have the most antioxidant capacity and antidiabetic effects compared to other okra parts, likely to be attributed to their higher levels of polyphenolic compounds. Similarly, okra Seeds also reduced vascular inflammation by downregulating TNFα-stimulated VCAM-1 and SELE expression. Furthermore, metabolite profiling by LC/MS also provided evidence of the cytoprotective effect of okra Seeds in endothelial cells. Therefore, the use of PHWE may be an alternative approach for the environmentally friendly extraction and evaluation of plant extracts for functional food applications.

Fructus mori L. polysaccharide-iron chelates formed by self-embedding with iron(iii) as the core exhibit good antioxidant activity

2019 ◽  
Vol 10 (6) ◽  
pp. 3150-3160 ◽  
Author(s):  
Jiaqi Zhang ◽  
Chun Chen ◽  
Xiong Fu
Keyword(s):  
Antioxidant Activity ◽  
Column Chromatography ◽  
Extraction Method ◽  
Deae Cellulose ◽  
Morus Alba ◽  
Hot Water ◽  
Water Extraction ◽  
Iron Chelates ◽  
Hot Water Extraction ◽  
The Core

Mulberry fruit polysaccharide (MFP) was obtained from Morus alba L. by a hot water extraction method, and mulberry polysaccharide fractions named MFP1, MFP2 and MFP3 were isolated by DEAE cellulose-52 column chromatography.

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