General baseline toxicity QSAR for nonpolar, polar and ionisable chemicals and their mixtures in the bioluminescence inhibition assay with Aliivibrio fischeri

2017 ◽  
Vol 19 (3) ◽  
pp. 414-428 ◽  
Author(s):  
Beate I. Escher ◽  
Andreas Baumer ◽  
Kai Bittermann ◽  
Luise Henneberger ◽  
Maria König ◽  
...  
Keyword(s):  
Distribution Ratio ◽  
Water Distribution ◽  
Qsar Model ◽  
Inhibition Assay ◽  
Microtox Assay ◽  
Bioluminescence Inhibition ◽  
Aliivibrio Fischeri ◽  
Acids And Bases ◽  
Bioluminescence Inhibition Assay

A general QSAR model for the Microtox assay with the ionisation-corrected liposome–water distribution ratio is applicable to diverse chemicals including acids and bases.

Vibrio fischeri bioluminescence inhibition assay for ecotoxicity assessment: A review

2018 ◽  
Vol 626 ◽  
pp. 1295-1309 ◽  
Author(s):  
Mazhar Abbas ◽  
Muhammad Adil ◽  
Syed Ehtisham-ul-Haque ◽  
Bushra Munir ◽  
Muhammad Yameen ◽  
...  
Keyword(s):  
Vibrio Fischeri ◽  
Inhibition Assay ◽  
Bioluminescence Inhibition ◽  
Bioluminescence Inhibition Assay

scholarly journals Bioluminescence Inhibition Assay for the Detection of Hydroxylated Polychlorinated Biphenyls

2012 ◽  
Vol 84 (18) ◽  
pp. 7648-7655 ◽  
Author(s):  
Krystal Teasley Hamorsky ◽  
C. Mark Ensor ◽  
Emre Dikici ◽  
Patrizia Pasini ◽  
Leonidas Bachas ◽  
...  
Keyword(s):  
Polychlorinated Biphenyls ◽  
Inhibition Assay ◽  
Hydroxylated Polychlorinated Biphenyls ◽  
Bioluminescence Inhibition ◽  
Bioluminescence Inhibition Assay

scholarly journals Comparative Studies on the pH Dependence of DOWof Microcystin-RR and -LR Using LC-MS

2011 ◽  
Vol 11 ◽  
pp. 20-26 ◽  
Author(s):  
Gaodao Liang ◽  
Ping Xie ◽  
Jun Chen ◽  
Ting Yu
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Physicochemical Properties ◽  
Distribution Ratio ◽  
High Performance ◽  
Water Distribution ◽  
Ph Dependence ◽  
Remarkable Difference ◽  
The Difference

Microcystins (MCs) are well known worldwide as hepatotoxins produced by cyanobacteria, but little is known about the physicochemical properties of these compounds. The dependence of the n-octanol/water distribution ratio (DOW) of MC-RR and -LR to pH was measured by high-performance liquid chromatography combined with mass spectrometry (LC-MS). There was a remarkable difference in such relationships between MC-RR and -LR. The log DOWof MC-LR decreased from 1.63 at pH 1.0 to -1.26 at pH 6.5, and stabilized between -1.04 and -1.56 at a pH of 6.5~12.0; log DOWof MC-RR varied between -1.24 and -0.67 at a pH of 1.00~4.00, and stabilized between -1.20 and -1.54 at a pH of 4.00~12.00. The difference of hydrophobicity in acidic condition between MC-RR and -LR is important, not only for the analytical method of both toxins, but perhaps also for understanding the difference of toxicity to animals between the two toxins.

scholarly journals Locomotor behavior of Neocaridina palmata: a study with leachates from UV-weathered microplastics

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12442
Author(s):  
Kristina Klein ◽  
Sebastian Heß ◽  
Ulrike Schulte-Oehlmann ◽  
Jörg Oehlmann
Keyword(s):  
Chemical Compounds ◽  
Biodegradable Material ◽  
Safe Alternative ◽  
Test Parameters ◽  
Microtox Assay ◽  
Aliivibrio Fischeri ◽  
The Individual ◽  
Recycled Low Density Polyethylene

Weathering of plastics leads to the formation of increasingly smaller particles with the release of chemical compounds. The latter occurs with currently unknown environmental impacts. Leachate-induced effects of weathered microplastics (MPs) are therefore of increasing concern. To investigate the toxicity of the chemical mixtures from such plastics, we exposed the freshwater shrimp Neocaridina palmata to enriched leachates from unweathered and artificially weathered (UV-A/B light) MPs (≤1 mm) from recycled low-density polyethylene (LDPE-R) pellets and from a biodegradable, not fully bio-based starch blend (SB) foil. We analyzed the individual locomotor activity (moved distance and frozen events) on day 1, 3, 7 and 14 of exposure to five leachate concentrations equivalent to 0.40–15.6 g MPs L−1, representing the upper scale of MPs that have been found in the environment. The median moved distance did not change as a function of concentration, except for the unweathered SB treatment on day 14 that indicated hyperactivity with increasing concentrations. Significant impacts were solely detected for few concentrations and exposure days. Generally, no consistent trend was observed across the experiments. We further assessed the baseline toxicity of the samples in the Microtox assay and detected high bioluminescence inhibitions of the bacterium Aliivibrio fischeri. This study demonstrates that neither the recycled nor the biodegradable material are without impacts on test parameters and therefore cannot be seen as safe alternative for conventional plastics regarding the toxicity. However, the observed in vitro toxicity did not result in substantial effects on the behavior of shrimps. Overall, we assume that the two endpoints examined in the atyid shrimp N. palmata were not sensitive to chemicals leaching from plastics or that effects on the in vivo level affect other toxic endpoints which were not considered in this study.

scholarly journals Preliminary ecotoxicity assessment of selected flavour and fragrance compounds using Microtox assay

2019 ◽  
Vol 100 ◽  
pp. 00036
Author(s):  
Wojciech Kołodyński ◽  
Katarzyna Piekarska ◽  
Daniel Strub
Keyword(s):  
Marine Bacteria ◽  
Vibrio Fischeri ◽  
Inorganic Compounds ◽  
Chemical Compounds ◽  
Potential Threat ◽  
Chemical Substances ◽  
Microtox Assay ◽  
Bioluminescence Inhibition ◽  
The Many ◽  
Fragrance Compounds

The bioluminescence inhibition bioassay using marine bacteria Vibrio fischeri is widely used as a tool to assess the toxicity of chemical compounds, because of the many benefits comprising cost and time effectiveness, rapidness, sensitivity, and ease of operation. In addition, the test is used for various types of organic and inorganic compounds. Due to the insolubility of organic compounds in water, a suitable solubilizer should be applied. The ecotoxicity of the solvent should be negligible in relation to marine bacteria. On account of superior human activities the synthesis of new chemical substances is inextricably linked to the emergence of chemical compounds that are a potential threat on environment. A Microtox system utilizing the 81.9% Basic Test with 14 dilutions was applied to pre-evaluate the ecotoxicity of β-cyclocitral oxime, citronellal oxime and perillaldehyde oxime. Substances solutions with an initial concentration of 0.036% in DMSO were prepared. The preliminary results show that the studied scent compounds are characterized by quite high toxicity. In order to confirm the received ecotoxicity values, it is necessary to carry out additional surveys using another range of concentrations and biotests on organisms at each trophic level.

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