Metabolization and degradation kinetics of the urban-use pesticide fipronil by white rot fungus Trametes versicolor

2016 ◽  
Vol 18 (10) ◽  
pp. 1256-1265 ◽  
Author(s):  
Jordyn M. Wolfand ◽  
Gregory H. LeFevre ◽  
Richard G. Luthy

The urban-use pesticide, fipronil, is metabolized to novel transformation products by white rot fungusT. versicolor.

2020 ◽  
pp. 1-10
Author(s):  
Wittawat Toomsan ◽  
Pinthita Mungkarndee ◽  
Sophon Boonlue ◽  
Nguyen Thanh Giao ◽  
Sumana Siripattanakul-Ratpukdi

This study was attempted to use laccase extracted from white rot fungus to remove melanoidin in the ethanol production wastewater. The isolated fungus producing the highest laccase was identified as Megaspororia sp. The highest degradation efficiencies of the purified and crude laccases were 48.00% and 44.60%, respectively. Both degradation kinetics well fit Michaelis-Menten model. The Michaelis constant (Km) and maximum rate of reaction (Vmax) were 0.82% melanoidin and 0.0045% melanoidin h-1 for the degradation by the purified laccase and 0.71% melanoidin and 0.0037% melanoidin h-1 for the degradation by the crude laccase. Turnover number (Kcat) of purified and crude laccases were 0.00023 and 0.00019% melanoidin U-1 h-1, respectively. Catalytic efficiency (Kcat/Km) of purified and crude laccases were 0.00028 and 0.00027 U-1 h-1, respectively. The affinity of the crude laccase was slightly higher because of its non-specificity. Kcat and Kcat/Km of the purified laccase were higher than the crude laccase. Proposed potential degradation result showed that laccase could oxidize CH3, carbonyl groups, haloalkanes (C–H), C–O and C–N bondings which probably caused decolorization of melanoidin in wastewater. Thus, the purified and crude laccases can be used to decolorize melanoidin-containing wastewater from ethanol industries. As the attempt to use purified laccase consumed times and costs especially in purification steps, the crude laccase can be used to degrade color of melanoidin in wastewater with only 3.4% lower than the purified laccase.


BioResources ◽  
2010 ◽  
Vol 6 (1) ◽  
pp. 59-69
Author(s):  
Seyyed Khalil Hosseini Hashemi ◽  
Ahmad Jahan Latibari

Walnut (Juglans regia L.) heartwood extractives were identified and their potential for protection of poplar wood was evaluated. Test specimens were prepared from poplar wood (Populus nigra L.) to meet BS 838:1961 requirements. Samples were impregnated with heartwood extractive solution (1.5, 2.5, and 3.5% w/w in ethanol-toluene), followed by 5 hours vacuum desiccator technique to reach complete saturation. Impregnated specimens were exposed to white-rot fungus (Trametes versicolor) for 14 weeks according to BS 838:1961 applying the kolle-flask method. The weight loss of samples was determined after exposure to white-rot fungus. The highest weight loss (36.96%) was observed for untreated control samples and the lowest weight loss (30.40%) was measured in samples treated with 1.5% extractives solution. The analyses of the extracts using GC/MS indicated that major constituents are benzoic acid,3,4,5-tri(hydroxyl) and gallic acid (44.57 %). The two toxic components in the heartwood are juglone (5.15 %) and 2,7-dimethylphenantheren (5.81 %).


2019 ◽  
Vol 33 (8) ◽  
pp. 1048-1060 ◽  
Author(s):  
Hamid R Taghiyari ◽  
Ehsan Bari ◽  
Asghar Sistani ◽  
Mohammad Najafian ◽  
Mohammad Ali Tajick Ghanbary ◽  
...  

The present study investigated the effects of exposure of bamboo–plastic composites to three aggressive fungi species on mass loss values. Polyvinyl chloride was used as the synthetic matrix of the composite. Three mixing combinations were used for bamboo–polymer composites, namely 40/60, 50/50, and 60/40. The injection-molded process was employed to produce the composites. Specimens were prepared according to modified European standard specifications (EN-113) to be exposed to white-, brown-, and soft-rot fungi each month for 4 months. The results indicated that the soft-rot fungus ( Chaetomium globosum) generally caused higher mass losses in all bamboo/plastic combination ratios. Brown- and soft-rot fungi demonstrated different mass losses on different combination ratios. However, the white-rot fungus ( Trametes versicolor) caused nearly the same mass losses on all three combination ratios. Moreover, T. versicolor was highly significant with respect to mass loss and moisture content. It can therefore be concluded that the T. versicolor has a different decay metabolism when compared to the brown- and soft-rot fungus used in this study.


2015 ◽  
Vol 77 (1) ◽  
pp. 28-38 ◽  
Author(s):  
Wan Nurul Izyani Wan Mohd Zawawi ◽  
Azmi Fadziyana Mansor ◽  
Nurul Sakinah Othman ◽  
Nur Atikah Mohidem ◽  
Nik Ahmad Nizam Nik Malek ◽  
...  

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