Electron ionization mass spectrometric analysis of air- and moisture-sensitive organometallic compounds

2016 ◽  
Vol 45 (39) ◽  
pp. 15552-15556 ◽  
Author(s):  
Johanne Penafiel ◽  
Amelia V. Hesketh ◽  
Ori Granot ◽  
J. Scott McIndoe
Keyword(s):  
Volatile Compounds ◽  
Organometallic Compounds ◽  
Electron Ionization ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Method ◽  
Spectrometric Analysis ◽  
Ionization Mass ◽  
Mass Spectrometric Method ◽  
Moisture Sensitive

Electron ionization (EI) is a reliable mass spectrometric method for the analysis of the vast majority of thermally stable and volatile compounds.

scholarly journals Optimization of the Protocol for Isolation of Exosome and Secretome of CACO-2 Colorectal Adenocarcinoma Cells from Conditioned Media for Subsequent Mass Spectrometric Analysis

2020 ◽  
Vol 3 (1) ◽  
pp. e00118
Author(s):  
N.A. Shushkova ◽  
S.E. Novikova ◽  
T.E. Farafonova ◽  
V.G. Zgoda
Keyword(s):  
Colorectal Adenocarcinoma ◽  
Mass Spectrometric ◽  
Conditioned Media ◽  
Mass Spectrometric Analysis ◽  
Secreted Proteins ◽  
Spectrometric Method ◽  
Spectrometric Analysis ◽  
Mass Spectrometric Method ◽  
Adenocarcinoma Cells ◽  
Tandem Mass Spectrometric

The conditioned media in which the tumor cells are cultured represents a model object for studying of secretome and proteomic composition of exosomes. This pool of proteins is of particular interest in terms of the search for potential markers of malignant diseases. The efficiency of the isolation of exosomes and secretome from the cell culture media largely determines the success of their analysis by the mass spectrometric method. In this paper, we have applied various approaches to isolate secretome and exosomes originated from Caco-2 colorectal adenocarcinoma cells. The combination of ultrafiltration and centrifugation provided a deep proteomic analysis of the secretome and exosomes obtained from the one initial volume of conditioned medium without the addition of fetal bovine serum. Applying tandem mass spectrometric analysis we have identified 436 secreted proteins. In exosomes, the characteristic proteins ALIX, CD63, syntenin, lactadherin (MFGE8) were identified. Using targeted mass spectrometry, the exosome markers CD82, CD9 and HSPA8 were determined at the levels of 0.08 ± 0.03 fmol/μg, 0.13 ± 0.03 fmol/μg and 2.6 ± 0.02 fmol/μg of total protein, respectively. Among the secreted proteins, there were many markers associated with tumor progression and metastasis, such as DAG1, PODXL, LRRN4, TGFBI, IL6ST, DSC1, DSG1, and NPC2.

Electrospray ionization mass spectrometric analysis of phospholipids of Escherichia coli

1996 ◽  
Vol 20 (1) ◽  
pp. 233-238 ◽  
Author(s):  
G. Sweetman ◽  
M. Trinei ◽  
J. Modha ◽  
J. Kusel ◽  
P. Freestone ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Electrospray Ionization ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
Ionization Mass

Direct analysis of herbicides by paper spray ionization mass spectrometry

2015 ◽  
Vol 7 (23) ◽  
pp. 9808-9816 ◽  
Author(s):  
Steven L. Reeber ◽  
Sneha Gadi ◽  
Sung-Ben Huang ◽  
Gary L. Glish
Keyword(s):  
Mass Spectrometry ◽  
Environmental Samples ◽  
Direct Analysis ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
Ionization Mass ◽  
Paper Spray ◽  
Paper Spray Ionization ◽  
Rapid Mass

Paper spray ionization enables the rapid mass spectrometric analysis of environmental samples without the use of chromatography or sample cleanup techniques.

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