External surface and pore mouth catalysis in hydrolysis of inulin over zeolites with different micropore topologies and mesoporosities

2017 ◽  
Vol 7 (5) ◽  
pp. 1153-1166 ◽  
Author(s):  
Su Cheun Oh ◽  
Thien Nguyendo ◽  
Yao He ◽  
Amanda Filie ◽  
Yiqing Wu ◽  
...  
Keyword(s):  
External Surface ◽  
Zeolite Catalysts ◽  
Inulin Hydrolysis ◽  
Pore Mouth ◽  
Hydrolysis Of

External surface and pore mouth catalysis for inulin hydrolysis over zeolite catalysts.

scholarly journals Production, characterization and application of inulinase from fungal endophyte CCMB 328

2012 ◽  
Vol 84 (2) ◽  
pp. 443-454 ◽  
Author(s):  
Diego S. Nascimento ◽  
Gildomar Valasques Junior ◽  
Pedro Fernandes ◽  
Geise C.A. Ribeiro ◽  
Danyo M. Lima ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Yeast Extract ◽  
Arid Region ◽  
Culture Medium ◽  
Fungal Endophyte ◽  
Initial Concentration ◽  
Inulin Hydrolysis ◽  
Semi Arid Region ◽  
Hydrolysis Of ◽  
Semi Arid

Inulinase (β-2,1-D- fructan fructanohydrolase), EC 3.2.1.7, targets the β-2,1 linkage of inulin, a polyfructan consisting of linear β-2,1 linked fructose, and hydrolyzes it into fructose. This use provides an alternative to produce fructose syrup through the hydrolysis of inulin. The objective of this work was to study the production, characterization and applications of inulinases from the fungal endophyte CCMB 328 isolated from the Brazilian semi-arid region. Response Surface Methodology (RSM) was employed to evaluate the effect of variables (concentration of glucose and yeast extract), on secreted inulinase activities detected in the culture medium and also in the inulin hydrolysis. The results showed that the best conditions for inulinase production by CCMB 328 are 9.89 g / L for glucose and 1.09 g / L for yeast extract. The concentration of 0.20 mol/L of NaCl and KCl increased the activity of inulinase from CCMB 328 by approximately 63% and 37%, respectively. The results also showed that the inulinase has potential for inulin hydrolysis, whose conversion yields roughly 72.48 % for an initial concentration of inulin at 1% (w/v).

Catalytic hydrolysis of HCN on ZSM-5 modified by Fe or Nb for HCN removal: surface species and performance

RSC Advances ◽  
2016 ◽  
Vol 6 (112) ◽  
pp. 111389-111397 ◽  
Author(s):  
Zhongxian Song ◽  
Qiulin Zhang ◽  
Ping Ning ◽  
Yu Wang ◽  
Yankang Duan ◽  
...  
Keyword(s):  
Zeolite Catalysts ◽  
Catalytic Hydrolysis ◽  
Surface Species ◽  
And Performance ◽  
Hydrolysis Of

Interactions present between Fe and Nb species resulted in larger amounts of isolated Fe3+, Nb5+, oligomeric FexOy and NbxOy clusters on zeolite catalysts, resulting in improved HCN hydrolysis.

Catalytic consequences of micropore topology, mesoporosity, and acidity on the hydrolysis of sucrose over zeolite catalysts

2014 ◽  
Vol 4 (9) ◽  
pp. 3064 ◽  
Author(s):  
Yao He ◽  
Thomas C. Hoff ◽  
Laleh Emdadi ◽  
Yiqing Wu ◽  
Judicael Bouraima ◽  
...  
Keyword(s):  
Zeolite Catalysts ◽  
Hydrolysis Of

scholarly journals Production, Characterization and Application of Inulinase from Pseudozyma sp. CCMB 300.

2014 ◽  
Vol 4 (2) ◽  
pp. 382-392
Author(s):  
Sandra A. Assis ◽  
Diego Sampaio Nascimento ◽  
Danyo M. Lima ◽  
Gildomar Valasques Junior ◽  
Rodrigo de Queiroz Oliveira ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Statistical Method ◽  
Response Surface ◽  
Arid Region ◽  
Initial Concentration ◽  
Inulin Hydrolysis ◽  
Optimum Ph ◽  
Semi Arid Region ◽  
Hydrolysis Of ◽  
Semi Arid

The use of inulinases provides an alternative way to obtain fructose syrup through the hydrolysis of inulin. The objective of this work was to study the production, characterization and application of an inulinase from Pseudozyma sp CCMB 300 isolated from Brazilian semi-arid region. The higher production of inulinase in a medium containing 0.86 g L-1 yeast extract and 14.54 g L-1 glucose using response surface methodology (RSM). This statistical method was also used to access the optimum pH (8.28) and temperature (54ºC) for fermentative inulinase production. The results showed that the inulinase has potential for inulin hydrolysis, where a conversion yields of roughly 87.39 % for an initial concentration of inulin of 1% (w/v).

scholarly journals PRODUCTION OF OLIGOFRUCTOSE SYRUP BY ENZYMATIC HYDROLYSIS FROM INULIN-CONTAINING CHICORY SYRUP

Food systems ◽  
2020 ◽  
Vol 3 (3) ◽  
pp. 20-24
Author(s):  
T. S. Puchkova ◽  
D. M. Pikhalo ◽  
O. M. Karasyova
Keyword(s):  
Enzymatic Hydrolysis ◽  
Ion Exchange ◽  
Ph Value ◽  
Optimal Conditions ◽  
Carbohydrate Composition ◽  
Inulin Hydrolysis ◽  
Stage Scheme ◽  
Hydrolysis Of ◽  
Dry Substance

The purpose of the work is to study the enzymatic hydrolysis of inulin-containing syrups of various purification degrees from chicory chips in the production of oligofructose to be used in dietary, diabetic and health-preventive nutrition products. It has been determined that ion exchange purification of the syrup is necessary for hydrolysis. Individual stages of ion-exchange purification are specified using a two-stage scheme: К1-А1-К2-А2 and an additional stage on the “Macronet” sorbent MN200 to stabilize pH and remove bitterness taste. Requirements for the quality of syrup for hydrolysis have been developed: pH value — 4.5–5.0; chromaticity — not more than 0.5 units opt. den.; ash — not more than 0.2%; protein — 0.5%; no bitterness taste. Optimal conditions for hydrolysis of inulin-containing syrup have been established using Novozim 960 endoinulinase (Denmark): temperature — 55–58 °C; pH — 4.7–5.2; DS (dry substance) — 19%; preparation dosage — 0.4 units. INU/g of syrup DS; time 20–24 h. A sample of oligofructose syrup was obtained using the preparation “Novozim 960.” The carbohydrate composition of oligofructose after the inulin hydrolysis was determined: fructooligosaccharides (FOS) — 70.12%; oligofructosides — 24.79%; disaccharides — 2.11%; fructose — 2.98%. Requirements for carbohydrate composition of oligofructose obtained by enzymatic hydrolysis of inulin-containing syrup have been developed: sum total of FOS and oligosaccharides — not less than 93%, sum total of di- and monosaccharides — not more than 7%.

scholarly journals Quantification of external surface and pore mouth acid sites in unit-cell thick pillared MFI and pillared MWW zeolites

2017 ◽  
Vol 241 ◽  
pp. 43-51 ◽  
Author(s):  
Yiqing Wu ◽  
Laleh Emdadi ◽  
Dongchang Qin ◽  
Junyan Zhang ◽  
Dongxia Liu
Keyword(s):  
External Surface ◽  
Acid Sites ◽  
Unit Cell ◽  
Pore Mouth

scholarly journals Production and action pattern of inulinase from Aspergillus Niger-245: hydrolysis of inulin from several sources

1998 ◽  
Vol 29 (4) ◽  
pp. 301-306 ◽  
Author(s):  
Vinícius D’Arcadia Cruz ◽  
Juliana Gisele Belote ◽  
Márcia Zilioli Belline ◽  
Rubens Cruz
Keyword(s):  
Aspergillus Niger ◽  
Carbon Sources ◽  
Jerusalem Artichoke ◽  
Soil Samples ◽  
Helianthus Tuberosus ◽  
Action Mechanism ◽  
Great Capacity ◽  
Inulin Hydrolysis ◽  
Optimum Ph ◽  
Hydrolysis Of

A strain of Aspergillus niger isolated from soil samples showed great capacity to produce extracellular inulinase. Although the enzyme has been synthesized in presence of monosaccharides, sucrose and sugar cane molasse, the productivity was significantly higher (p<0.05) when the microorganism was inoculated in media formulated with dahlia extract and pure inulin, as carbon sources. With regard to the nitrogen source, the best results were obtained with casein and other sources of proteic nitrogen, comparatively to the mineral nitrogen. However, statistic significance (p<0.01) only was found between the productivity obtained in the medium prepared with casein and ammonium sulphate. The optimum pH of the purified enzyme for inulin hydrolysis was found between 4.0 and 4.5 and the optimun temperature at 60oC. When treated by 30 minutes in this temperature no loss of activity was observed. The enzyme showed capacity to hydrolyse sucrose, raffinose and inulin from which it liberated only fructose units showing, therefore, an exo-action mechanism. Acting on inulins from several sources, the enzyme showed larger hydrolysis speed on the polissaccharide from chicory (Cichorium intibus), comparatively, to the inulins from dahlia (Dahlia pinnata) and Jerusalem artichoke (Helianthus tuberosus) roots.

Investigation on Acid Hydrolysis of Inulin: A Response Surface Methodology Approach

2009 ◽  
Vol 5 (3) ◽  
Author(s):  
Ebrahim Eskandari Nasab ◽  
Mehran Habibi-Rezaei ◽  
Afshin Khaki ◽  
Mohammad Balvardi
Keyword(s):  
Response Surface Methodology ◽  
Acid Hydrolysis ◽  
Response Surface ◽  
Reducing Sugars ◽  
Experimental Program ◽  
Central Composite Rotatable Design ◽  
Alkaline Ph ◽  
Inulin Hydrolysis ◽  
Hydrolysis Of ◽  
Central Composite

In this study, the acid hydrolysis of inulin was investigated as a function of three variables: pH, temperature and time. Inulin hydrolysis was detected by measurement of reducing sugars, using Dinitro Salicylic acid (DNS) method. The central composite rotatable design (CCRD) was applied to design an experimental program to model the effects of acidic and alkaline pH on the inulin hydrolysis. Additionally, response surface methodology (RSM) was utilized for data analysis. The statistical analysis of the results confirmed that pH, temperature and time are significant variables at acidic pH, whereas at alkaline pH, these variables are insignificant. The maximum amount of inulin hydrolysis obtained at the pH < 2, temperature > 90°C and the time of 1 hrs.

scholarly journals Hydrolysis of cellulose to glucose by supercritical water and silver mesoporous zeolite catalysts

Cellulose ◽  
2019 ◽  
Vol 26 (4) ◽  
pp. 2471-2485 ◽  
Author(s):  
S. G. Aspromonte ◽  
A. Romero ◽  
A. V. Boix ◽  
E. Alonso
Keyword(s):  
Supercritical Water ◽  
Zeolite Catalysts ◽  
Mesoporous Zeolite ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of
Sign in / Sign up

Export Citation Format

Share Document