Reply to the ‘Comment on “Magnetic-field-enabled resolution enhancement in super-resolution imaging”' by Bergmann et al., Physical Chemistry Chemical Physics, 2017, 19, DOI: 10.1039/C6CP05108A

2017 ◽  
Vol 19 (6) ◽  
pp. 4891-4892 ◽  
Author(s):  
Min Zhang ◽  
Junling Chen ◽  
Zhiyuan Tian ◽  
Hongda Wang
Keyword(s):  
Physical Chemistry ◽  
Magnetic Field ◽  
Single Molecule ◽  
Resolution Enhancement ◽  
Super Resolution ◽  
Chemical Physics ◽  
Resolution Imaging ◽  
Positive Effect ◽  
The Magnetic Field

By ensemble and single molecule experiments, we confirmed that the magnetic field unequivocally exerted a positive effect on the fluorescence of dyes in dSTORM imaging.

Comment on “Magnetic-field-enabled resolution enhancement in super-resolution imaging” by M. Zhang et al., Phys. Chem. Chem. Phys., 2015, 17, 6722–6727

2017 ◽  
Vol 19 (6) ◽  
pp. 4887-4890 ◽  
Author(s):  
Stephan Bergmann ◽  
Viola Mönkemöller ◽  
Thomas Huser
Keyword(s):  
Magnetic Field ◽  
Resolution Enhancement ◽  
Super Resolution ◽  
Chem Phys ◽  
Enhanced Fluorescence ◽  
Organic Fluorophores ◽  
Resolution Imaging ◽  
Phys Chem Chem Phys

Spectral fluorimetry demonstrates that common organic fluorophores such as Alexa 647 exhibit no magnetic field enhanced fluorescence in the absence/presence of a strong magnet.

Magnetic-field-enabled resolution enhancement in super-resolution imaging

2015 ◽  
Vol 17 (10) ◽  
pp. 6722-6727 ◽  
Author(s):  
Min Zhang ◽  
Junling Chen ◽  
Jing Gao ◽  
Zhiyong Wang ◽  
Haijiao Xu ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Fluorescence Intensity ◽  
Resolution Enhancement ◽  
Super Resolution ◽  
Resolution Imaging ◽  
Localization Precision

Magnetic field could increase dye's fluorescence intensity and number of photons, thus better localization precision of super-resolution imaging was achieved.

scholarly journals Comparing lifeact and phalloidin for super-resolution imaging of actin in fixed cells

PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0246138
Author(s):  
Hanieh Mazloom-Farsibaf ◽  
Farzin Farzam ◽  
Mohamadreza Fazel ◽  
Michael J. Wester ◽  
Marjolein B. M. Meddens ◽  
...  
Keyword(s):  
Single Molecule ◽  
Cell Biology ◽  
Cell Movement ◽  
Super Resolution ◽  
Actin Binding ◽  
Thin Filaments ◽  
Reversible Binding ◽  
Multiple Regions ◽  
Resolution Imaging ◽  
Division Cell

Visualizing actin filaments in fixed cells is of great interest for a variety of topics in cell biology such as cell division, cell movement, and cell signaling. We investigated the possibility of replacing phalloidin, the standard reagent for super-resolution imaging of F-actin in fixed cells, with the actin binding peptide ‘lifeact’. We compared the labels for use in single molecule based super-resolution microscopy, where AlexaFluor 647 labeled phalloidin was used in a dSTORM modality and Atto 655 labeled lifeact was used in a single molecule imaging, reversible binding modality. We found that imaging with lifeact had a comparable resolution in reconstructed images and provided several advantages over phalloidin including lower costs, the ability to image multiple regions of interest on a coverslip without degradation, simplified sequential super-resolution imaging, and more continuous labeling of thin filaments.

Single molecule Coordinate and Height super-resolution Imaging with Dithering and Orientation (CHIDO)

2020 ◽  
Author(s):  
Luis A. Alemán-Castañeda ◽  
Valentina Curcio ◽  
Thomas G. Brown ◽  
Sophie Brasselet ◽  
Miguel A. Alonso
Keyword(s):  
Single Molecule ◽  
Super Resolution ◽  
Resolution Imaging

scholarly journals Millimeter and Microwave Activity of the Sun

1990 ◽  
Vol 142 ◽  
pp. 457-465 ◽  
Author(s):  
M. R. Kundu ◽  
S. M. White
Keyword(s):  
Magnetic Field ◽  
Gamma Rays ◽  
Solar Flares ◽  
Gamma Ray ◽  
High Spatial Resolution ◽  
High Resolution Imaging ◽  
The Sun ◽  
Millimeter Wavelengths ◽  
Resolution Imaging ◽  
The Magnetic Field

The emission of solar flares at millimeter wavelengths is of great interest both in its own right and because it is generated by the energetic electrons which also emit gamma rays. Since high-resolution imaging at gamma-ray energies is not presently possible, millimeter observations can act as a substitute. Except for that class of flares known as gamma-ray flares the millimetric emission is optically thin. It can be used as a powerful diagnostic of the energy distribution of electrons in solar flares and its evolution, and of the magnetic field. We have carried out high-spatial-resolution millimeter observations of solar flares this year using the Berkeley-Illinois-Maryland Array (BIMA), and report on the preliminary results in this paper (Kundu et al 1990; White et al 1990). We also report some recent results obtained from multifrequency observations using the VLA (White et al 1990).

scholarly journals Fast, Simultaneous Multiple Fluorophore Fitting in Single Molecule Super-Resolution Imaging

2011 ◽  
Vol 100 (3) ◽  
pp. 349a
Author(s):  
Fang Huang ◽  
Samantha L. Schwartz ◽  
Jason M. Byars ◽  
Keith A. Lidke
Keyword(s):  
Single Molecule ◽  
Super Resolution ◽  
Resolution Imaging ◽  
Fitting In

scholarly journals Titelbild: SLAP: Small Labeling Pair for Single-Molecule Super-Resolution Imaging (Angew. Chem. 35/2015)

2015 ◽  
Vol 127 (35) ◽  
pp. 10175-10175
Author(s):  
Ralph Wieneke ◽  
Anika Raulf ◽  
Alina Kollmannsperger ◽  
Mike Heilemann ◽  
Robert Tampé
Keyword(s):  
Single Molecule ◽  
Super Resolution ◽  
Resolution Imaging

scholarly journals PSF shaping using adaptive optics for three-dimensional single-molecule super-resolution imaging and tracking

2012 ◽  
Vol 20 (5) ◽  
pp. 4957 ◽  
Author(s):  
Ignacio Izeddin ◽  
Mohamed El Beheiry ◽  
Jordi Andilla ◽  
Daniel Ciepielewski ◽  
Xavier Darzacq ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
Single Molecule ◽  
Three Dimensional ◽  
Super Resolution ◽  
Resolution Imaging
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