Precipitation SELEX: identification of DNA aptamers for calcium phosphate materials synthesis

2017 ◽  
Vol 53 (6) ◽  
pp. 1092-1095 ◽  
Author(s):  
K. R. Baillargeon ◽  
K. Meserve ◽  
S. Faulkner ◽  
S. Watson ◽  
H. Butts ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Dna Aptamers ◽  
Mineral Formation ◽  
Materials Synthesis ◽  
Phosphate Mineral

DNA aptamers that enhance calcium phosphate mineral formation were identified using a novel precipitation SELEX method.

Intracellular mineralization of Bacterionema matruchotii

1981 ◽  
Vol 27 (3) ◽  
pp. 267-270 ◽  
Author(s):  
S. W. Ooi ◽  
A. C. Smillie ◽  
T. B. Kardos ◽  
M. G. Shepherd
Keyword(s):  
Crystal Growth ◽  
Calcium Phosphate ◽  
Intracellular Calcium ◽  
Electron Micrographs ◽  
Solid Phase ◽  
Calcium Hydroxyapatite ◽  
Mineral Formation ◽  
Phosphate Mineral ◽  
Calcium And Phosphorus ◽  
Phosphorus Levels

Intracellular calcium phosphate mineral formation in Bacterionema matruchotii has been examined relative to two established points: the product of calcium and phosphorus levels at which spontaneous precipitation occurs (Ca × P), 50 (mg %)2, and the product at which calcium hydroxyapatite itself induces crystal growth, 35 (mg %)2. The extent of intracellular mineralization in the defined calcifying medium was determined analytically after washing the cells. Electron micrographs of B. matruchotii cells revealed needlelike crystals of mineral, typical of calcium hydroxyapatite. Bacterionema matruchotii was found to be an efficient nucleator and can bring about the formation of solid phase at a Ca × P product as low as 35 (mg %)2.

scholarly journals Lipid composition modulates ATP hydrolysis and calcium phosphate mineral propagation by TNAP-harboring proteoliposomes

2020 ◽  
Vol 691 ◽  
pp. 108482
Author(s):  
B.Z. Favarin ◽  
M. Bolean ◽  
A.P. Ramos ◽  
A. Magrini ◽  
N. Rosato ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Lipid Composition ◽  
Atp Hydrolysis ◽  
Phosphate Mineral

scholarly journals MMP20 Proteolysis of Native Amelogenin Regulates Mineralization In Vitro

2016 ◽  
Vol 95 (13) ◽  
pp. 1511-1517 ◽  
Author(s):  
S.Y. Kwak ◽  
Y. Yamakoshi ◽  
J.P. Simmer ◽  
H.C. Margolis
Keyword(s):  
Calcium Phosphate ◽  
Full Length ◽  
Protein Solutions ◽  
Mineral Formation ◽  
C Protein ◽  
Ordered Arrays ◽  
Transmission Electron ◽  
Precursor Phase ◽  
Lower Capacity

Recent studies have shown that native phosphorylated full-length porcine amelogenin (P173) and its predominant cleavage product (P148) can inhibit spontaneous calcium phosphate formation in vitro by stabilizing an amorphous calcium phosphate (ACP) precursor phase. Since full-length amelogenin undergoes proteolysis by matrix metalloproteinase 20 (MMP20, enamelysin) soon after secretion, the present study was conducted to assess the effect of amelogenin proteolysis on calcium phosphate formation. Calcium and phosphate were sequentially added to protein solutions without and with added MMP20 (ratio = 200:1) under physiological-like conditions of ionic strength (163 mM) in 50 mM Tris-HCl (pH 7.4) at 37 °C. Protein degradation with time was assessed by gel-electrophoresis, and mineral products formed were characterized by transmission electron microscopy (TEM). MMP20 was found to cleave P173 to primarily generate P148, along with P162, P46-148, and P63/64-148. In sharp contrast, MMP20 did not cleave P148. In addition, the formation of well-aligned bundles of enamel-like hydroxyapatite (HA) crystals was promoted in the presence of P173 with added MMP20, while only ACP particles were seen in the absence of MMP20. Although P148 was found to have a somewhat lower capacity to stabilize ACP and prevent HA formation compared with P173 in the absence of MMP20, essentially no HA formation was observed in the presence of somewhat higher concentrations of P148 regardless of MMP20 addition, due to the lack of observed protein proteolysis. Present findings suggest that ACP transformation to ordered arrays of enamel crystals may be regulated in part by the proteolysis of full-length native amelogenin, while the predominant amelogenin degradation product in developing enamel (e.g., P148) primarily serves to prevent uncontrolled mineral formation during the secretory stage of amelogenesis.

scholarly journals MORE ON DEVELOPING FISH PASTE WITH IMPROVED BIOLOGICAL VALUE

2020 ◽  
Vol 2020 (3) ◽  
pp. 134-143
Author(s):  
Maria Evgenievna Tsibizova
Keyword(s):  
Chemical Composition ◽  
Calcium Phosphate ◽  
Grass Carp ◽  
Raw Materials ◽  
Food Additives ◽  
Experimental Studies ◽  
Mineral Supplement ◽  
Mineral Supplements ◽  
Phosphate Mineral ◽  
Physiological Group

In the context of the organization of proper nutrition of the population of Russia, regardless of belonging to any physiological group, taking into account the eating behavior of a modern person, the research has been carried out to expand the range of fish pastes by modifying their recipes. The goal has been achieved by combining raw materials of different origins and reducing the mass fraction of table salt. Experimental studies were carried out to substantiate the recipe compositions of pastes based on grass carp meat, the optimal proportion of calcium-phosphate mineral supplements was defined, a comparative analysis of organoleptic indicators of quality, chemical composition and energy value of the obtained products was conducted. It has been stated that the introduction of 2.5% of phosphate-calcium food additives into paste recipes No. 4 and 5 did not adversely affect the organoleptic quality indicators. The pastes produced according to recipes No. 4 and No. 5, which include grass carp meat (48%), chicken liver (10%), vegetable components (17%), calcium-phosphate mineral supplement (2,5%) and flavoring substances, can be recommended for mass nutrition of the population of Russia. The ratio of these components provides the high organoleptic properties and improved nutritional value. The inclusion of fish pastes enriched with vegetable components, chicken by-products, phosphate-calcium mineral supplements, differing in chemical composition, in the diet allows balancing the diet for proteins, fats, amino acids, vitamins, macro- and micronutrients and preventing from eating disorders and, in the result, reducing morbidity of population in the Russian Federation.

scholarly journals The Influences of Magnesium upon Calcium Phosphate Mineral Formation and Structure as Monitored by X-ray and Vibrational Spectroscopy

Soil Systems ◽  
2020 ◽  
Vol 4 (1) ◽  
pp. 8
Author(s):  
David M. Hilger ◽  
Jordan G. Hamilton ◽  
Derek Peak
Keyword(s):  
Calcium Phosphate ◽  
Calcareous Soils ◽  
X Ray Diffraction ◽  
Edge Structure ◽  
X Ray ◽  
Transmission Electron ◽  
Phosphate Mineral ◽  
Local Bonding ◽  
Wide Range ◽  
High Concentrations

Calcium phosphate minerals are typically the solubility-limiting phase for phosphate in calcareous soils. Magnesium (Mg), despite being present in high concentrations in calcareous soils, has been largely neglected in the study of formation and stabilization of soil phosphate minerals due to the high solubility of pure Mg phosphate phases. In this study, a series of four common calcium and magnesium phosphate minerals, hydroxyapatite/bobierrite and brushite/newberyite were synthesized in the presence of widely varying Mg concentrations to examine the effects of Mg substitution upon the local bonding environment and overall structure of the precipitates. Phosphorus K-edge X-Ray absorption near edge structure (XANES) and attenuated total reflectance Fourier transform infrared (ATR-FTIR) provide insight into the local coordination environment, whereas synchrotron powder X-Ray diffraction (SP-XRD) and transmission electron microscopy (TEM) were used for structural analysis. In acidic to neutral pH, Mg-bearing brushite phases formed over a wide range of Ca:Mg ratios. In neutral to high pH systems, a short-range order amorphous calcium phosphate (ACP) with a local structure analogous with hydroxyapatite precipitated for a wide range of Ca to Mg ratios. It can be inferred that the presence of Mg in soils leads to stabilization of metastable phases: via cation substitution in brushite and via poisoning of crystal growth propagation for hydroxyapatite.

Nonenzymatic Transformation of Amorphous CaCO3into Calcium Phosphate Mineral after Exposure to Sodium Phosphate in Vitro: Implications for in Vivo Hydroxyapatite Bone Formation

ChemBioChem ◽  
2015 ◽  
Vol 16 (9) ◽  
pp. 1323-1332 ◽  
Author(s):  
Werner E. G. Müller ◽  
Meik Neufurth ◽  
Jian Huang ◽  
Kui Wang ◽  
Qingling Feng ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Formation ◽  
Sodium Phosphate ◽  
Phosphate Mineral
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