Two-photon fluorescence imaging of sialylated glycans in vivo based on a sialic acid imprinted conjugated polymer nanoprobe

2016 ◽  
Vol 52 (97) ◽  
pp. 13991-13994 ◽  
Author(s):  
Wei Zhang ◽  
Junqing Kang ◽  
Ping Li ◽  
Lu Liu ◽  
Hui Wang ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Fluorescence Imaging ◽  
Conjugated Polymer ◽  
Fluorescence Properties ◽  
Two Photon ◽  
Specific Recognition ◽  
Recognition Ability ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

We have designed and synthesized an SA-imprinted conjugated polymer nanoprobe with two-photon fluorescence properties, which exhibits specific recognition ability to the target SA and has been used for monitoring sialylated glycan levels selectively in vivo.

Unprecedentedly High Tissue Penetration Capability of Co-Assembled Nanosystems for Two-Photon Fluorescence Imaging In Vivo

2015 ◽  
Vol 3 (5) ◽  
pp. 646-651 ◽  
Author(s):  
Pei-Pei Yang ◽  
Yang Yang ◽  
Yu-Juan Gao ◽  
Yi Wang ◽  
Ju-Chen Zhang ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Tissue Penetration ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Color‐Tunable Light‐up Bioorthogonal Probes for In Vivo Two‐Photon Fluorescence Imaging

2020 ◽  
Vol 26 (20) ◽  
pp. 4576-4582 ◽  
Author(s):  
Yandong Dou ◽  
Yajun Wang ◽  
Yukun Duan ◽  
Bin Liu ◽  
Qinglian Hu ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Two Photon ◽  
Color Tunable ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

A series of Zn(ii) terpyridine complexes with enhanced two-photon-excited fluorescence for in vitro and in vivo bioimaging

2015 ◽  
Vol 3 (36) ◽  
pp. 7213-7221 ◽  
Author(s):  
Qiong Zhang ◽  
Xiaohe Tian ◽  
Zhangjun Hu ◽  
Caroline Brommesson ◽  
Jieying Wu ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Cross Sections ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

TPA cross sections are enhanced for the complexes containing D–A type ligandL1.1exhibits specificity in two-photon fluorescence imaging.

Simultaneous atomic-force and two-photon fluorescence imaging of biological specimens in vivo

2004 ◽  
Vol 99 (4) ◽  
pp. 235-245 ◽  
Author(s):  
Claudiu C. Gradinaru ◽  
Peter Martinsson ◽  
Thijs J. Aartsma ◽  
Thomas Schmidt
Keyword(s):  
Fluorescence Imaging ◽  
Two Photon ◽  
Atomic Force ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

scholarly journals Longitudinal in vivo two-photon fluorescence imaging

2014 ◽  
Vol 522 (8) ◽  
pp. 1708-1727 ◽  
Author(s):  
Sarah E. Crowe ◽  
Graham C.R. Ellis-Davies
Keyword(s):  
Fluorescence Imaging ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

In vivo two-photon fluorescence imaging with Cr:forsterite lasers using transgenic lines tagged by HcRed

2005 ◽  
Author(s):  
Tsung-Han Tsai ◽  
Szu-Yu Chen ◽  
Shih-Peng Tai ◽  
Cheng-Yung Lin ◽  
Huai-Jen Tsai ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Two Photon ◽  
Transgenic Lines ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

scholarly journals Volumetric two-photon fluorescence imaging of live neurons using a multimode optical fiber

2020 ◽  
Author(s):  
Raphaël Turcotte ◽  
Carla C. Schmidt ◽  
Martin J. Booth ◽  
Nigel J. Emptage
Keyword(s):  
Fluorescence Imaging ◽  
Optical Fibers ◽  
Continuous Wave ◽  
Brain Slices ◽  
Brain Regions ◽  
Laser Source ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Multimode optical fibers (MMFs), combined with wavefront control methods, have achieved minimally-invasive in vivo imaging of neurons in deep-brain regions with diffraction-limited spatial resolution. Here, we report a method for volumetric two-photon fluorescence imaging with a MMF-based system requiring a single transmission matrix measurement. Central to this method is the use of a laser source able to generate both continuous wave light and femtosecond pulses. The chromatic spreading of pulses generated an axially elongated excitation focus, which we used to demonstrate volumetric imaging of neurons and their dendrites in live rat brain slices through a 60 μm-core MMF.

Simple method to improve spatial resolution for in vivo two-photon fluorescence imaging

2015 ◽  
Vol 54 (34) ◽  
pp. 10044 ◽  
Author(s):  
Gerardo Estrada ◽  
Christopher Beetle ◽  
James Schummers
Keyword(s):  
Fluorescence Imaging ◽  
Spatial Resolution ◽  
Simple Method ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

scholarly journals Fluorescent AIE-Active Materials for Two-Photon Bioimaging Applications

2020 ◽  
Vol 8 ◽  
Author(s):  
Qing Lu ◽  
Cheng-Juan Wu ◽  
Zhiqiang Liu ◽  
Guangle Niu ◽  
Xiaoqiang Yu
Keyword(s):  
Fluorescence Imaging ◽  
Ex Vivo ◽  
Tissue Imaging ◽  
Active Materials ◽  
Two Photon ◽  
Real Time Visualization ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Fluorescence imaging has been widely used as a powerful tool for in situ and real-time visualization of important analytes and biological events in live samples with remarkably high selectivity, sensitivity, and spatial resolution. Compared with one-photon fluorescence imaging, two-photon fluorescence imaging exhibits predominant advantages of minimal photodamage to samples, deep tissue penetration, and outstanding resolution. Recently, the aggregation-induced emission (AIE) materials have become a preferred choice in two-photon fluorescence biological imaging because of its unique bright fluorescence in solid and aggregate states and strong resistance to photobleaching. In this review, we will exclusively summarize the applications of AIE-active materials in two-photon fluorescence imaging with some representative examples from four aspects: fluorescence detection, in vitro cell imaging, ex vivo tissue imaging, and in vivo vascular imaging. In addition, the current challenges and future development directions of AIE-active materials for two-photon bioimaging are briefly discussed.

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