Synthesis of precipitating chromogenic/fluorogenic β-glucosidase/β-galactosidase substrates by a new method and their application in the visual detection of foodborne pathogenic bacteria

2017 ◽  
Vol 53 (1) ◽  
pp. 103-106 ◽  
Author(s):  
Xianhu Wei ◽  
Qingping Wu ◽  
Jumei Zhang ◽  
Youxiong Zhang ◽  
Weipeng Guo ◽  
...  
Keyword(s):  
Efficient Method ◽  
Pathogenic Bacteria ◽  
Visual Detection ◽  
New Method ◽  
Foodborne Pathogenic Bacteria

Some indoxyl β-glycosides and novel precipitating fluorogenic β-glucosides were synthesized by a new efficient method and applied for detecting bacteria.

scholarly journals Antimicrobial Efficacy and Spectrum of Phosphorous-Fluorine Co-Doped TiO2 Nanoparticles on the Foodborne Pathogenic Bacteria Campylobacter jejuni, Salmonella Typhimurium, Enterohaemorrhagic E. coli, Yersinia enterocolitica, Shewanella putrefaciens, Listeria monocytogenes and Staphylococcus aureus

Foods ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1786
Author(s):  
György Schneider ◽  
Bettina Schweitzer ◽  
Anita Steinbach ◽  
Botond Zsombor Pertics ◽  
Alysia Cox ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Campylobacter Jejuni ◽  
Food Industry ◽  
Pathogenic Bacteria ◽  
Antibacterial Activities ◽  
Tio2 Nps ◽  
E Coli ◽  
Foodborne Pathogenic Bacteria ◽  
And Staphylococcus Aureus ◽  
Co Doped

Contamination of meats and meat products with foodborne pathogenic bacteria raises serious safety issues in the food industry. The antibacterial activities of phosphorous-fluorine co-doped TiO2 nanoparticles (PF-TiO2) were investigated against seven foodborne pathogenic bacteria: Campylobacter jejuni, Salmonella Typhimurium, Enterohaemorrhagic E. coli, Yersinia enterocolitica, Shewanella putrefaciens, Listeria monocytogenes and Staphylococcus aureus. PF-TiO2 NPs were synthesized hydrothermally at 250 °C for 1, 3, 6 or 12 h, and then tested at three different concentrations (500 μg/mL, 100 μg/mL, 20 μg/mL) for the inactivation of foodborne bacteria under UVA irradiation, daylight exposure or dark conditions. The antibacterial efficacies were compared after 30 min of exposure to light. Distinct differences in the antibacterial activities of the PF-TiO2 NPs, and the susceptibilities of tested foodborne pathogenic bacterium species were found. PF-TiO2/3 h and PF-TiO2/6 h showed the highest antibacterial activity by decreasing the living bacterial cell number from ~106 by ~5 log (L. monocytogenes), ~4 log (EHEC), ~3 log (Y. enterolcolitca, S. putrefaciens) and ~2.5 log (S. aureus), along with complete eradication of C. jejuni and S. Typhimurium. Efficacy of PF-TiO2/1 h and PF-TiO2/12 h NPs was lower, typically causing a ~2–4 log decrease in colony forming units depending on the tested bacterium while the effect of PF-TiO2/0 h was comparable to P25 TiO2, a commercial TiO2 with high photocatalytic activity. Our results show that PF-co-doping of TiO2 NPs enhanced the antibacterial action against foodborne pathogenic bacteria and are potential candidates for use in the food industry as active surface components, potentially contributing to the production of meats that are safe for consumption.

scholarly journals Incidence and characterisation of Bacillus cereus bacteriophages from Thua Nao, a Thai fermented soybean product

2021 ◽  
Vol 12 (1) ◽  
pp. 85-93
Author(s):  
Wallapat Phongtang ◽  
Ekachai Chukeatirote
Keyword(s):  
Bacillus Cereus ◽  
Food Industry ◽  
Pathogenic Bacteria ◽  
Morphological Analysis ◽  
Food Poisoning ◽  
Fermented Soybean ◽  
Foodborne Pathogenic Bacteria ◽  
Ph Range ◽  
Potential Use

Abstract Bacillus cereus is considered to be an important food poisoning agent causing diarrhea and vomiting. In this study, the occurrence of B. cereus bacteriophages in Thai fermented soybean products (Thua Nao) was studied using five B. cereus sensu lato indicator strains (four B. cereus strains and one B. thuringiensis strain). In a total of 26 Thua Nao samples, there were only two bacteriophages namely BaceFT01 and BaceCM02 exhibiting lytic activity against B. cereus. Morphological analysis revealed that these two bacteriophages belonged to the Myoviridae. Both phages were specific to B. cereus and not able to lyse other tested bacteria including B. licheniformis and B. subtilis. The two phages were able to survive in a pH range between 5 and 12. However, both phages were inactive either by treatment of 50°C for 2 h or exposure of UV for 2 h. It should be noted that both phages were chloroform-insensitive, however. This is the first report describing the presence of bacteriophages in Thua Nao products. The characterization of these two phages is expected to be useful in the food industry for an alternative strategy including the potential use of the phages as a biocontrol candidate against foodborne pathogenic bacteria.

A New Method for Railway Fastener Detection Using the Symmetrical Image and Its EA-HOG Feature

2019 ◽  
Vol 34 (02) ◽  
pp. 2055006
Author(s):  
Jiajia Liu ◽  
Jianying Yuan ◽  
Yongfang Jia
Keyword(s):  
Computer Vision ◽  
Sparse Representation ◽  
Efficient Method ◽  
Important Task ◽  
New Method ◽  
Automatic Inspection ◽  
Test Image ◽  
Recognition Result ◽  
The Status ◽  
Railway Operation

Railway fastener recognition and detection is an important task for railway operation safety. However, the current automatic inspection methods based on computer vision can effectively detect the intact or completely missing fasteners, but they have weaker ability to recognize the partially worn ones. In our method, we exploit the EA-HOG feature fastener image, generate two symmetrical images of original test image and turn the detection of the original test image into the detection of two symmetrical images, then integrate the two recognition results of symmetrical image to reach exact recognition of original test image. The potential advantages of the proposed method are as follows: First, we propose a simple yet efficient method to extract the fastener edge, as well as the EA-HOG feature of the fastener image. Second, the symmetry images indeed reflect some possible appearance of the fastener image which are not shown in the original images, these changes are helpful for us to judge the status of the symmetry samples based on the improved sparse representation algorithm and then obtain an exact judgment of the original test image by combining the two corresponding judgments of its symmetry images. The experiment results show that the proposed approach achieves a rather high recognition result and meets the demand of railway fastener detection.

Photodynamic inactivation as an emergent strategy against foodborne pathogenic bacteria in planktonic and sessile states

2018 ◽  
Vol 44 (6) ◽  
pp. 667-684 ◽  
Author(s):  
Alex Fiori Silva ◽  
Anabela Borges ◽  
Efstathios Giaouris ◽  
Jane Martha Graton Mikcha ◽  
Manuel Simões
Keyword(s):  
Pathogenic Bacteria ◽  
Photodynamic Inactivation ◽  
Emergent Strategy ◽  
Foodborne Pathogenic Bacteria
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