Controlling molecularity and stability of hydrogen bonded G-quadruplexes by modulating the structure's periphery

2016 ◽  
Vol 52 (74) ◽  
pp. 11112-11115 ◽  
Author(s):  
Keith B. Sutyak ◽  
Peter Y. Zavalij ◽  
Michael L. Robinson ◽  
Jeffery T. Davis
Keyword(s):  
Structure And Stability ◽  
Quadruplex Structure ◽  
G Quadruplex ◽  
Hydrogen Bonded

Identity of 5′-ester controls G-quadruplex structure and stability.

scholarly journals The Effects of Molecular Crowding on the Structure and Stability of G-Quadruplexes with an Abasic Site

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Takeshi Fujimoto ◽  
Shu-ichi Nakano ◽  
Daisuke Miyoshi ◽  
Naoki Sugimoto
Keyword(s):  
Site Effects ◽  
Enthalpy Change ◽  
Molecular Crowding ◽  
Abasic Site ◽  
Structure And Stability ◽  
Quadruplex Structure ◽  
Abasic Sites ◽  
Crowding Effects ◽  
G Quadruplex ◽  
Quadruplex Formation

Both cellular environmental factors and chemical modifications critically affect the properties of nucleic acids. However, the structure and stability of DNA containing abasic sites under cell-mimicking molecular crowding conditions remain unclear. Here, we investigated the molecular crowding effects on the structure and stability of the G-quadruplexes including a single abasic site. Structural analysis by circular dichroism showed that molecular crowding by PEG200 did not affect the topology of the G-quadruplex structure with or without an abasic site. Thermodynamic analysis further demonstrated that the degree of stabilization of the G-quadruplex by molecular crowding decreased with substitution of an abasic site for a single guanine. Notably, we found that the molecular crowding effects on the enthalpy change for G-quadruplex formation had a linear relationship with the abasic site effects depending on its position. These results are useful for predicting the structure and stability of G-quadruplexes with abasic sites in the cell-mimicking conditions.

scholarly journals G-quadruplex structure and stability illuminated by 2-aminopurine phasor plots

2012 ◽  
Vol 40 (9) ◽  
pp. 4203-4215 ◽  
Author(s):  
Robert Buscaglia ◽  
David M. Jameson ◽  
Jonathan B. Chaires
Keyword(s):  
Structure And Stability ◽  
Quadruplex Structure ◽  
G Quadruplex

A novel pyrimidine tetrad contributing to stabilize tetramolecular G-quadruplex structures

2016 ◽  
Vol 14 (10) ◽  
pp. 2938-2943 ◽  
Author(s):  
V. Esposito ◽  
A. Pepe ◽  
R. Filosa ◽  
L. Mayol ◽  
A. Virgilio ◽  
...  
Keyword(s):  
Quadruplex Structure ◽  
G Quadruplex ◽  
Hydrogen Bonded

5-Amino-2′-deoxyuridine forms an eight hydrogen-bonded tetrad stabilizing a parallel G-quadruplex structure more efficiently than tetrads formed by 5-bromo-2′-deoxyuridine and thymidine.

Design and Synthesis of an Expanded Porphyrin That Has Selectivity for the c-MYC G-Quadruplex Structure

2005 ◽  
Vol 127 (9) ◽  
pp. 2944-2959 ◽  
Author(s):  
Jeyaprakashnarayanan Seenisamy ◽  
Sridevi Bashyam ◽  
Vijay Gokhale ◽  
Hariprasad Vankayalapati ◽  
Daekyu Sun ◽  
...  
Keyword(s):  
Design And Synthesis ◽  
Quadruplex Structure ◽  
G Quadruplex ◽  
Expanded Porphyrin

scholarly journals Structure and Stability of a Dimeric G-Quadruplex Formed by Cyclic Oligonucleotides

2010 ◽  
Vol 2010 ◽  
pp. 1-6 ◽  
Author(s):  
Joan Casals ◽  
Júlia Viladoms ◽  
Enrique Pedroso ◽  
Carlos González
Keyword(s):  
Telomeric Repeat ◽  
High Melting ◽  
Structure And Stability ◽  
Dimeric Structure ◽  
Nmr Data ◽  
High Melting Temperature ◽  
Sodium Nmr ◽  
G Quadruplex ◽  
Global Topology ◽  
Quadruplex Formation

We have studied the structure and stability of the cyclic dodecamer d<pGGGTTAGGGTTA>, containing two copies of the human telomeric repeat. In the presence of sodium, NMR data are consistent with a dimeric structure of the molecule in which two cycles self-associate forming a quadruplex with three guanine tetrads connected by edgewise loops. The two macrocycles are arranged in a parallel way, and the dimeric structure exhibits a high melting temperature. These results indicate that cyclization of the phosphodiester chain does not prevent quadruplex formation, although it affects the global topology of the quadruplex.

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