Synthesis of photoactivatable azido-acyl caged oxazine fluorophores for live-cell imaging

2016 ◽  
Vol 52 (60) ◽  
pp. 9442-9445 ◽  
Author(s):  
Andrew V. Anzalone ◽  
Zhixing Chen ◽  
Virginia W. Cornish
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Living Cells ◽  
Live Cell

A new cell-permeable caged oxazine fluorophore was synthesized for protein specific labeling and photoactivation in living cells.

Use of rhodamine-allyl Schiff base in chemodosimetric processes for total palladium estimation and application in live cell imaging

2018 ◽  
Vol 42 (21) ◽  
pp. 17351-17358 ◽  
Author(s):  
Anup Kumar Bhanja ◽  
Snehasis Mishra ◽  
Ketaki Kar ◽  
Kaushik Naskar ◽  
Suvendu Maity ◽  
...  
Keyword(s):  
Schiff Base ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Living Cells ◽  
Live Cell ◽  
Raw 264.7 ◽  
Macrophage Cells ◽  
Raw 264.7 Macrophage ◽  
Raw 264.7 Macrophage Cells

An allyl-rhodamine Schiff base shows excellent palladium sensitivity (LOD, 95 nM) irrespective of Pd(0,ii,iv) and practical applicability is judged in living cells of RAW 264.7 (macrophage) cells.

scholarly journals Illuminating the Sites of Enterovirus Replication in Living Cells by Using a Split-GFP-Tagged Viral Protein

mSphere ◽  
2016 ◽  
Vol 1 (4) ◽  
Author(s):  
H. M. van der Schaar ◽  
C. E. Melia ◽  
J. A. C. van Bruggen ◽  
J. R. P. M. Strating ◽  
M. E. D. van Geenen ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Lipid Composition ◽  
Viral Protein ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Living Cells ◽  
Live Cell ◽  
Genome Replication ◽  
Fluorescent Reporter ◽  
Unique Protein

ABSTRACT Enteroviruses induce the formation of membranous structures (replication organelles [ROs]) with a unique protein and lipid composition specialized for genome replication. Electron microscopy has revealed the morphology of enterovirus ROs, and immunofluorescence studies have been conducted to investigate their origin and formation. Yet, immunofluorescence analysis of fixed cells results in a rather static view of RO formation, and the results may be compromised by immunolabeling artifacts. While live-cell imaging of ROs would be preferred, enteroviruses encoding a membrane-anchored viral protein fused to a large fluorescent reporter have thus far not been described. Here, we tackled this constraint by introducing a small tag from a split-GFP system into an RO-resident enterovirus protein. This new tool bridges a methodological gap by circumventing the need for immunolabeling fixed cells and allows the study of the dynamics and formation of enterovirus ROs in living cells. Like all other positive-strand RNA viruses, enteroviruses generate new organelles (replication organelles [ROs]) with a unique protein and lipid composition on which they multiply their viral genome. Suitable tools for live-cell imaging of enterovirus ROs are currently unavailable, as recombinant enteroviruses that carry genes that encode RO-anchored viral proteins tagged with fluorescent reporters have not been reported thus far. To overcome this limitation, we used a split green fluorescent protein (split-GFP) system, comprising a large fragment [strands 1 to 10; GFP(S1-10)] and a small fragment [strand 11; GFP(S11)] of only 16 residues. The GFP(S11) (GFP with S11 fragment) fragment was inserted into the 3A protein of the enterovirus coxsackievirus B3 (CVB3), while the large fragment was supplied by transient or stable expression in cells. The introduction of GFP(S11) did not affect the known functions of 3A when expressed in isolation. Using correlative light electron microscopy (CLEM), we showed that GFP fluorescence was detected at ROs, whose morphologies are essentially identical to those previously observed for wild-type CVB3, indicating that GFP(S11)-tagged 3A proteins assemble with GFP(S1-10) to form GFP for illumination of bona fide ROs. It is well established that enterovirus infection leads to Golgi disintegration. Through live-cell imaging of infected cells expressing an mCherry-tagged Golgi marker, we monitored RO development and revealed the dynamics of Golgi disassembly in real time. Having demonstrated the suitability of this virus for imaging ROs, we constructed a CVB3 encoding GFP(S1-10) and GFP(S11)-tagged 3A to bypass the need to express GFP(S1-10) prior to infection. These tools will have multiple applications in future studies on the origin, location, and function of enterovirus ROs. IMPORTANCE Enteroviruses induce the formation of membranous structures (replication organelles [ROs]) with a unique protein and lipid composition specialized for genome replication. Electron microscopy has revealed the morphology of enterovirus ROs, and immunofluorescence studies have been conducted to investigate their origin and formation. Yet, immunofluorescence analysis of fixed cells results in a rather static view of RO formation, and the results may be compromised by immunolabeling artifacts. While live-cell imaging of ROs would be preferred, enteroviruses encoding a membrane-anchored viral protein fused to a large fluorescent reporter have thus far not been described. Here, we tackled this constraint by introducing a small tag from a split-GFP system into an RO-resident enterovirus protein. This new tool bridges a methodological gap by circumventing the need for immunolabeling fixed cells and allows the study of the dynamics and formation of enterovirus ROs in living cells.

A fluorescence ‘turn-on’ chemodosimeter for the specific detection of Pd2+by a rhodamine appended Schiff base and its application in live cell imaging

2017 ◽  
Vol 46 (28) ◽  
pp. 9245-9252 ◽  
Author(s):  
Anup Kumar Bhanja ◽  
Snehasis Mishra ◽  
Krishna Das Saha ◽  
Chittaranjan Sinha
Keyword(s):  
Schiff Base ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Bond Cleavage ◽  
Living Cells ◽  
Live Cell ◽  
Specific Detection ◽  
Physiological Conditions ◽  
Turn On ◽  
Fluorescence Turn On

Allylether-Rhodamine selectively invites Pd2+from mixture of other cations and Pd(0) in H2O-MeCN medium and exhibits maroon emission due to C(allyl)-O bond cleavage and opening of spirolactam ring. The LOD is 50 nM at pH 7. Imaging Pd2+species in living cells under physiological conditions is achieved.

A highly selective and sensitive fluorescent probe for Cu2+based on a novel naphthalimide–rhodamine platform and its application in live cell imaging

2017 ◽  
Vol 15 (18) ◽  
pp. 3947-3954 ◽  
Author(s):  
Chang Liu ◽  
Xiaojie Jiao ◽  
Song He ◽  
Liancheng Zhao ◽  
Xianshun Zeng
Keyword(s):  
Fluorescent Probe ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Living Cells ◽  
Live Cell ◽  
Ratiometric Fluorescent Probe

A bifluorophore-based ratiometric fluorescent probe has been developed. The probe is cell-permeable and can be used as a FRET-based ratiometric fluorescent probe for lysosomal Cu2+imaging in living cells.

scholarly journals An Optically Decodable Bead Array for Linking Imaging and Sequencing with Single-Cell Resolution

2018 ◽  
Author(s):  
Jinzhou Yuan ◽  
Jenny Sheng ◽  
Peter A. Sims
Keyword(s):  
Single Cell ◽  
Expression Profiling ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Living Cells ◽  
Live Cell ◽  
Proof Of Concept ◽  
Rna Seq ◽  
Phenotypic Analysis

AbstractOptically decodable beads link the identity of an analyte or sample to a measurement through an optical barcode, enabling libraries of biomolecules to be captured on beads in solution and decoded by fluorescence. This approach has been foundational to microarray, sequencing, and flow-based expression profiling technologies. We have combined microfluidics with optically decodable beads to link phenotypic analysis of living cells to sequencing. As a proof-of-concept, we applied this to demonstrate an accurate and scalable tool for connecting live cell imaging to single-cell RNA-Seq called Single Cell Optical Phenotyping and Expression (SCOPE-Seq).

Fluorogenic naked-eye sensing and live-cell imaging of cyanide by a hydrazine-functionalized CAU-10 metal–organic framework

CrystEngComm ◽  
2018 ◽  
Vol 20 (29) ◽  
pp. 4194-4201 ◽  
Author(s):  
Rana Dalapati ◽  
Soutick Nandi ◽  
Helge Reinsch ◽  
Bibhas K. Bhunia ◽  
Biman B. Mandal ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Metal Organic Framework ◽  
Living Cells ◽  
Live Cell ◽  
Naked Eye ◽  
Metal Organic ◽  
Organic Framework

A hydrazine-functionalized Al(iii) based metal–organic framework was utilized for the detection of lethal cyanide in water and in living cells.

scholarly journals Live-cell imaging of multiple endogenous mRNAs permits the direct observation of RNA granule dynamics

2018 ◽  
Vol 54 (52) ◽  
pp. 7151-7154 ◽  
Author(s):  
Kenji Yatsuzuka ◽  
Shin-ichi Sato ◽  
Kathleen Beverly Pe ◽  
Yousuke Katsuda ◽  
Ippei Takashima ◽  
...  
Keyword(s):  
Direct Observation ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Living Cells ◽  
Live Cell ◽  
Stress Granule ◽  
Granule Formation ◽  
Imaging Technology ◽  
Rna Imaging

A multicolor RNA imaging technology permits the direct observation of stress granule formation in living cells.

New Advances in Live-cell Imaging: Multiplexed Protein Activity Measurements and Correlational Studies of Rho GTPase Regulation in Living Cells.

2012 ◽  
Vol 18 (S2) ◽  
pp. 130-131
Author(s):  
L. Hodgson ◽  
D. Spiering ◽  
M. Sabouri ◽  
C. Der Mardirossian ◽  
G. Danuser ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Rho Gtpase ◽  
Living Cells ◽  
Live Cell ◽  
Protein Activity ◽  
Activity Measurements

Extended abstract of a paper presented at Microscopy and Microanalysis 2012 in Phoenix, Arizona, USA, July 29 – August 2, 2012.

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