Detecting 5-methylcytosine using an enzyme-free DNA strand exchange reaction without pretreatment under physiological conditions

2016 ◽  
Vol 52 (41) ◽  
pp. 6833-6836 ◽  
Author(s):  
Chen Xu ◽  
Jinjun Wu ◽  
Wenting Liu ◽  
Tingting Hong ◽  
Tianlu Wang ◽  
...  
Keyword(s):  
Exchange Reaction ◽  
Strand Exchange ◽  
Physiological Conditions ◽  
Free Dna ◽  
Dna Strand Exchange ◽  
Dna Strand

DNA strand exchange reaction activated by cationic comb-type copolymers having ureido groups

2014 ◽  
Vol 2 (10) ◽  
pp. 1480-1485 ◽  
Author(s):  
Naohiko Shimada ◽  
Wei Song ◽  
Atsushi Maruyama
Keyword(s):  
Exchange Reaction ◽  
Graft Copolymers ◽  
Strand Exchange ◽  
Dna Strand Exchange ◽  
Dna Strand

Ureido modification of cationic graft copolymers accelerated DNA strand exchange reaction relative to unmodified copolymers.

scholarly journals Improved RecA-assisted fluorescence assay for DNA strand exchange reaction

BioTechniques ◽  
2006 ◽  
Vol 40 (6) ◽  
pp. 736-738 ◽  
Author(s):  
Oleg Kaboev ◽  
Ludmila Luchkina ◽  
Valery Shalguev ◽  
Yury Andreichuk ◽  
Vladimir Kulikov ◽  
...  
Keyword(s):  
Exchange Reaction ◽  
Fluorescence Assay ◽  
Strand Exchange ◽  
Dna Strand Exchange ◽  
Dna Strand

scholarly journals Control of directionality in the DNA strand-exchange reaction catalysed by the tyrosine recombinase TnpI

2009 ◽  
Vol 38 (6) ◽  
pp. 2044-2056 ◽  
Author(s):  
Virginie Vanhooff ◽  
Christophe Normand ◽  
Christine Galloy ◽  
Anca M. Segall ◽  
Bernard Hallet
Keyword(s):  
Exchange Reaction ◽  
Strand Exchange ◽  
Tyrosine Recombinase ◽  
Dna Strand Exchange ◽  
Dna Strand

scholarly journals Swi5-Sfr1 protein stimulates Rad51-mediated DNA strand exchange reaction through organization of DNA bases in the presynaptic filament

2013 ◽  
Vol 42 (4) ◽  
pp. 2358-2365 ◽  
Author(s):  
Louise H. Fornander ◽  
Axelle Renodon-Cornière ◽  
Naoyuki Kuwabara ◽  
Kentaro Ito ◽  
Yasuhiro Tsutsui ◽  
...  
Keyword(s):  
Exchange Reaction ◽  
Linear Dichroism ◽  
Strand Exchange ◽  
Filament Axis ◽  
Double Stranded Dna ◽  
Dna Base ◽  
Dna Strand Exchange ◽  
Dna Strand ◽  
Base Orientation

Abstract The Swi5-Sfr1 heterodimer protein stimulates the Rad51-promoted DNA strand exchange reaction, a crucial step in homologous recombination. To clarify how this accessory protein acts on the strand exchange reaction, we have analyzed how the structure of the primary reaction intermediate, the Rad51/single-stranded DNA (ssDNA) complex filament formed in the presence of ATP, is affected by Swi5-Sfr1. Using flow linear dichroism spectroscopy, we observe that the nucleobases of the ssDNA are more perpendicularly aligned to the filament axis in the presence of Swi5-Sfr1, whereas the bases are more randomly oriented in the absence of Swi5-Sfr1. When using a modified version of the natural protein where the N-terminal part of Sfr1 is deleted, which has no affinity for DNA but maintained ability to stimulate the strand exchange reaction, we still observe the improved perpendicular DNA base orientation. This indicates that Swi5-Sfr1 exerts its activating effect through interaction with the Rad51 filament mainly and not with the DNA. We propose that the role of a coplanar alignment of nucleobases induced by Swi5-Sfr1 in the presynaptic Rad51/ssDNA complex is to facilitate the critical matching with an invading double-stranded DNA, hence stimulating the strand exchange reaction.

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