Maleic anhydride proton sponge as a novel MALDI matrix for the visualization of small molecules (<250 m/z) in brain tumors by routine MALDI ToF imaging mass spectrometry

2016 ◽  
Vol 52 (63) ◽  
pp. 9801-9804 ◽  
Author(s):  
M. Giampà ◽  
M. B. Lissel ◽  
T. Patschkowski ◽  
J. Fuchser ◽  
V. H. Hans ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Maleic Anhydride ◽  
Brain Tumors ◽  
Small Molecules ◽  
Imaging Mass Spectrometry ◽  
Proton Sponge ◽  
Maldi Matrix ◽  
Maldi Tof

A novel MALDI matrix MAPS, able to visualize deviating metabolism in glioma using a routine MALDI-ToF-MSI procedure, is presented.

scholarly journals Trypsin and MALDI matrix pre-coated targets simplify sample preparation for mapping proteomic distributions within biological tissues by imaging mass spectrometry

2016 ◽  
Vol 51 (12) ◽  
pp. 1168-1179 ◽  
Author(s):  
Faizan Zubair ◽  
Paul E. Laibinis ◽  
William G. Swisher ◽  
Junhai Yang ◽  
Jeffrey M. Spraggins ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Imaging Mass Spectrometry ◽  
Biological Tissues ◽  
Maldi Matrix

Methods for Human Olfactory Bulb Tissue Studies Using Peptide/Protein MALDI-TOF Imaging Mass Spectrometry (MALDI-IMS)

2017 ◽  
pp. 91-106 ◽  
Author(s):  
Ibon Iloro ◽  
Joaquín Fernández-Irigoyen ◽  
Iraide Escobes ◽  
Mikel Azkargorta ◽  
Enrique Santamaría ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Olfactory Bulb ◽  
Imaging Mass Spectrometry ◽  
Maldi Tof ◽  
Maldi Ims

scholarly journals Dereplikace látek a de novo charakterizace malých molekul z hmotnostních spekter

2022 ◽  
Vol 116 (1) ◽  
pp. 11-19
Author(s):  
Jiří Novák ◽  
Vladimír Havlíček
Keyword(s):  
Mass Spectrometry ◽  
Small Molecules ◽  
Mass Spectra ◽  
De Novo ◽  
Imaging Mass Spectrometry ◽  
Mass Spectrometry Data ◽  
Data Sets ◽  
Liquid Chromatography Mass Spectrometry ◽  
Imaging Mass Spectrometry Data

We describe the molecular dereplication principles and de novo characterization of small molecules obtained from liquid-chromatography mass spectrometry and imaging mass spectrometry data sets. Our methodology aims at supporting chemists and computer programmers to understand the hidden computing algorithms used for metabolomics mass spectrometry data processing. The approaches have been made available in the open-source tool CycloBranch. The presented tutorial extends the interpretation of mass spectra portfolia described in a series of papers published in Chemicke Listy, issues 2/2020 and 3/2020.

scholarly journals Mass spectrometric imaging of metabolites in kidney tissues from rats treated with furosemide

2016 ◽  
Vol 310 (11) ◽  
pp. F1317-F1327 ◽  
Author(s):  
Jin Woo Jung ◽  
Mi Suk Lee ◽  
Hyo-Jung Choi ◽  
Sunhee Jung ◽  
Yu-Jung Lee ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Spatial Distribution ◽  
Rat Kidney ◽  
Imaging Mass Spectrometry ◽  
Urine Osmolality ◽  
Free Access ◽  
Sprague Dawley ◽  
Fixed Amount ◽  
Maldi Tof ◽  
Relative Quantitation

In the kidney, metabolic processes are different among the cortex (COR), outer medulla (OM), and inner medulla (IM). Using matrix-assisted laser desorption/ionization (MALDI) and imaging mass spectrometry (IMS), we examined the change of metabolites in the COR, OM, and IM of the rat kidney after furosemide treatment compared with vehicle-treated controls. Osmotic minipumps were implanted in male Sprague-Dawley rats to deliver 12 mg·day−1·rat−1 of furosemide. Vehicle-treated ( n = 14) and furosemide-treated (furosemide rats, n = 15) rats in metabolic cages received a fixed amount of rat chow (15 g·220 g body wt−1·day−1 for each rat) with free access to water intake for 6 days. At day 6, higher urine output (32 ± 4 vs. 9 ± 1 ml/day) and lower urine osmolality (546 ± 44 vs. 1,677 ± 104 mosmol/kgH2O) were observed in furosemide rats. Extracts of COR, OM, and IM were analyzed by ultraperformance liquid chromatography coupled with quadrupole time-of-flight (TOF) mass spectrometry, where multivariate analysis revealed significant differences between the two groups. Several metabolites, including acetylcarnitine, betaine, carnitine, choline, and glycerophosphorylcholine (GPC), were significantly changed. The changes of metabolites were further identified by MALDI-TOF/TOF and IMS. Their spatial distribution and relative quantitation in the kidneys were analyzed by IMS. Carnitine compounds were increased in COR and IM, whereas carnitine and acetylcarnitine were decreased in OM. Choline compounds were increased in COR and OM but decreased in IM from furosemide rats. Betaine and GPC were decreased in OM and IM. Taken together, MALDI-TOF/TOF and IMS successfully provide the spatial distribution and relative quantitation of metabolites in the kidney.

N-doped graphene quantum dots as a novel highly-efficient matrix for the analysis of perfluoroalkyl sulfonates and other small molecules by MALDI-TOF MS

2017 ◽  
Vol 9 (13) ◽  
pp. 2014-2020 ◽  
Author(s):  
Ziyu Rao ◽  
Fanglan Geng ◽  
Yiqi Zhou ◽  
Dong Cao ◽  
Yuehui Kang
Keyword(s):  
Quantum Dots ◽  
Small Molecules ◽  
Small Molecule ◽  
Graphene Quantum Dots ◽  
Maldi Tof Ms ◽  
Maldi Matrix ◽  
Maldi Tof ◽  
Doped Graphene ◽  
Small Molecule Analysis ◽  
Tof Ms

N-doped graphene quantum dots (N-GQDs) were sythesized by a facile method and applied as a MALDI matrix for small-molecule analysis.

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