Dual-component collagenous peptide/reactive oligomer hydrogels as potential nerve guidance materials – from characterization to functionalization

2016 ◽  
Vol 4 (11) ◽  
pp. 1605-1621 ◽  
Author(s):  
C. Kohn ◽  
J. M. Klemens ◽  
C. Kascholke ◽  
N. S. Murthy ◽  
J. Kohn ◽  
...  
Keyword(s):  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerve Regeneration ◽  
Reactive Oligomer ◽  
Biological Performance ◽  
New Generation

Toward a new generation of improved nerve guidance conduits (NGCs), novel biomaterials are required to address pressing clinical shortcomings in peripheral nerve regeneration (PNR) and to promote biological performance.

scholarly journals Construction of Dual-Biofunctionalized Chitosan/Collagen Scaffolds for Simultaneous Neovascularization and Nerve Regeneration

Research ◽  
2020 ◽  
Vol 2020 ◽  
pp. 1-18
Author(s):  
Guicai Li ◽  
Qi Han ◽  
Panjian Lu ◽  
Liling Zhang ◽  
Yuezhou Zhang ◽  
...  
Keyword(s):  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Composite Scaffold ◽  
Peripheral Nerve Regeneration ◽  
Functional Restoration ◽  
Collagen Scaffolds ◽  
Repair Capacity ◽  
Solution Blending ◽  
Biological Performance

Biofunctionalization of artificial nerve implants by incorporation of specific bioactive factors has greatly enhanced the success of grafting procedures for peripheral nerve regeneration. However, most studies on novel biofunctionalized implants have emphasized the promotion of neuronal and axonal repair over vascularization, a process critical for long-term functional restoration. We constructed a dual-biofunctionalized chitosan/collagen composite scaffold with Ile-Lys-Val-Ala-Val (IKVAV) and vascular endothelial growth factor (VEGF) by combining solution blending, in situ lyophilization, and surface biomodification. Immobilization of VEGF and IKVAV on the scaffolds was confirmed both qualitatively by staining and quantitatively by ELISA. Various single- and dual-biofunctionalized scaffolds were compared for the promotion of endothelial cell (EC) and Schwann cell (SC) proliferation as well as the induction of angiogenic and neuroregeneration-associated genes by these cells in culture. The efficacy of these scaffolds for vascularization was evaluated by implantation in chicken embryos, while functional repair capacity in vivo was assessed in rats subjected to a 10 mm sciatic nerve injury. Dual-biofunctionalized scaffolds supported robust EC and SC proliferation and upregulated the expression levels of multiple genes and proteins related to neuroregeneration and vascularization. Dual-biofunctionalized scaffolds demonstrated superior vascularization induction in embryos and greater promotion of vascularization, myelination, and functional recovery in rats. These findings support the clinical potential of VEGF/IKVAV dual-biofunctionalized chitosan/collagen composite scaffolds for facilitating peripheral nerve regeneration, making it an attractive candidate for repairing critical nerve defect. The study may provide a critical experimental and theoretical basis for the development and design of new artificial nerve implants with excellent biological performance.

scholarly journals Dual-Component Gelatinous Peptide/Reactive Oligomer Formulations as Conduit Material and Luminal Filler for Peripheral Nerve Regeneration

2017 ◽  
Vol 18 (5) ◽  
pp. 1104 ◽  
Author(s):  
Caroline Kohn-Polster ◽  
Divya Bhatnagar ◽  
Derek Woloszyn ◽  
Matthew Richtmyer ◽  
Annett Starke ◽  
...  
Keyword(s):  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerve Regeneration ◽  
Reactive Oligomer

Biological performance of a novel biodegradable polyamidoamine hydrogel as guide for peripheral nerve regeneration

2011 ◽  
Vol 98A (1) ◽  
pp. 19-30 ◽  
Author(s):  
Valerio Magnaghi ◽  
Vincenzo Conte ◽  
Patrizia Procacci ◽  
Giorgio Pivato ◽  
Paolo Cortese ◽  
...  
Keyword(s):  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerve Regeneration ◽  
Biological Performance

Modern approaches to peripheral nerve regeneration after injury: the prospects of gene and cell therapy

2017 ◽  
Author(s):  
M. Karagyaur ◽  
P. Makarevich ◽  
E. Shevchenko ◽  
D. Stambolsky ◽  
N. Kalinina ◽  
...  
Keyword(s):  
Cell Therapy ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerve Regeneration ◽  
Gene And Cell Therapy

Oxidized Galectin-1 is an Essential Factor for Peripheral Nerve Regeneration

2005 ◽  
Vol 6 (4) ◽  
pp. 385-394 ◽  
Author(s):  
Hidenori Horie ◽  
Toshihiko Kadoya ◽  
Kazunori Sango ◽  
Mitsuhiro Hasegawa
Keyword(s):  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerve Regeneration ◽  
Essential Factor

Lumbar Dorsal Root Transection in Adult Mice to Study Peripheral Nerve Regeneration

BIO-PROTOCOL ◽  
2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Hyukmin Kim ◽  
Harun Noristani ◽  
Seung Han ◽  
Young-Jin Son
Keyword(s):  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Dorsal Root ◽  
Peripheral Nerve Regeneration ◽  
Adult Mice
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