scholarly journals Implications of direct amplification for measuring antimicrobial resistance using point-of-care devices

2017 ◽  
Vol 9 (8) ◽  
pp. 1229-1241 ◽  
Author(s):  
M. R. Williams ◽  
R. D. Stedtfeld ◽  
H. Waseem ◽  
T. Stedtfeld ◽  
B. Upham ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Point Of Care ◽  
Direct Amplification

The use of direct amplification combined with point-of-care technologies for measuring antimicrobial resistance could expedite diagnoses.

scholarly journals Emerging Options for the Diagnosis of Bacterial Infections and the Characterization of Antimicrobial Resistance

2021 ◽  
Vol 22 (1) ◽  
pp. 456
Author(s):  
Simone Rentschler ◽  
Lars Kaiser ◽  
Hans-Peter Deigner
Keyword(s):  
Antimicrobial Resistance ◽  
Bacterial Infections ◽  
Point Of Care ◽  
Rapid Identification ◽  
Adequate Treatment ◽  
Resistance Patterns ◽  
Detection And Diagnosis ◽  
Patient Prognosis ◽  
Point Of Care Detection

Precise and rapid identification and characterization of pathogens and antimicrobial resistance patterns are critical for the adequate treatment of infections, which represent an increasing problem in intensive care medicine. The current situation remains far from satisfactory in terms of turnaround times and overall efficacy. Application of an ineffective antimicrobial agent or the unnecessary use of broad-spectrum antibiotics worsens the patient prognosis and further accelerates the generation of resistant mutants. Here, we provide an overview that includes an evaluation and comparison of existing tools used to diagnose bacterial infections, together with a consideration of the underlying molecular principles and technologies. Special emphasis is placed on emerging developments that may lead to significant improvements in point of care detection and diagnosis of multi-resistant pathogens, and new directions that may be used to guide antibiotic therapy.

scholarly journals Microfluidics as an Emerging Platform for Tackling Antimicrobial Resistance (AMR): A Review

2020 ◽  
Vol 16 (1) ◽  
pp. 41-51 ◽  
Author(s):  
Sammer-ul Hassan ◽  
Xunli Zhang
Keyword(s):  
Antimicrobial Resistance ◽  
Bacterial Resistance ◽  
Point Of Care ◽  
Treatment Options ◽  
Cost Effective ◽  
Droplet Microfluidics ◽  
New Drugs ◽  
Testing Tools ◽  
Antibiotics Use ◽  
Increasing Demand

Background: Antimicrobial resistance (AMR) occurs when microbes become resistant to antibiotics causing complications and limited treatment options. AMR is more significant where antibiotics use is excessive or abusive and the strains of bacteria become resistant to antibiotic treatments. Current technologies for bacteria and its resistant strains identification and antimicrobial susceptibility testing (AST) are mostly central-lab based in hospitals, which normally take days to weeks to get results. These tools and procedures are expensive, laborious and skills based. There is an ever-increasing demand for developing point-of-care (POC) diagnostics tools for rapid and near patient AMR testing. Microfluidics, an important and fundamental technique to develop POC devices, has been utilized to tackle AMR in healthcare. This review mainly focuses on the current development in the field of microfluidics for rapid AMR testing. Method: Due to the limitations of conventional AMR techniques, microfluidic-based platforms have been developed for better understandings of bacterial resistance, smart AST and minimum inhibitory concentration (MIC) testing tools and development of new drugs. This review aims to summarize the recent development of AST and MIC testing tools in different formats of microfluidics technology. Results: Various microfluidics devices have been developed to combat AMR. Miniaturization and integration of different tools has been attempted to produce handheld or standalone devices for rapid AMR testing using different formats of microfluidics technology such as active microfluidics, droplet microfluidics, paper microfluidics and capillary-driven microfluidics. Conclusion: Current conventional AMR detection technologies provide time-consuming, costly, labor-intensive and central lab-based solutions, limiting their applications. Microfluidics has been developed for decades and the technology has emerged as a powerful tool for POC diagnostics of antimicrobial resistance in healthcare providing, simple, robust, cost-effective and portable diagnostics. The success has been reported in research articles; however, the potential of microfluidics technology in tackling AMR has not been fully achieved in clinical settings.

Core–shell nanoparticles as platform technologies for paper based point-of-care devices to detect antimicrobial resistance

2020 ◽  
Vol 8 (29) ◽  
pp. 6296-6306
Author(s):  
Kapil Punjabi ◽  
Rishi Rajat Adhikary ◽  
Aishani Patnaik ◽  
Prachi Bendale ◽  
Subhasini Singh ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Point Of Care ◽  
Core Shell ◽  
Shell Nanoparticles ◽  
Platform Technology ◽  
Development Of Instrument ◽  
Core Shell Nanoparticles

Development of instrument-free point-of-care devices comprising of core-shell nanoparticles as platform technology to make paper based device for detection of antimicrobial resistance in sample.

Innovations in point of care testing for bacterial infections: The Longitude Prize with Daniel Berman

2020 ◽  
Author(s):  
Daniel Berman
Keyword(s):  
Antimicrobial Resistance ◽  
Diagnostic Tests ◽  
Bacterial Infections ◽  
Point Of Care ◽  
Rapid Diagnostic Tests ◽  
Public Healthcare ◽  
Point Of Care Testing ◽  
Set Up ◽  
Insight Into

Antimicrobial resistance (AMR) is one of the most serious clinical and public healthcare challenges. In this video Daniel Berman,  Nesta Challenges, provides an overview of the Longitude Prize, why the prize was set up and what the prize hopes to achieve. Daniel also provides insight into some of the rapid diagnostic tests currently in the running for the £8 million prize.

scholarly journals Challenges in Microfluidic and Point-of-Care Phenotypic Antimicrobial Resistance Tests

2020 ◽  
Vol 6 ◽  
Author(s):  
Sarah H. Needs ◽  
Sultan I. Donmez ◽  
Stephanie P. Bull ◽  
Conor McQuaid ◽  
Helen M. I. Osborn ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Point Of Care ◽  
Resistance Tests

scholarly journals Leapfrogging laboratories: the promise and pitfalls of high-tech solutions for antimicrobial resistance surveillance in low-income settings

2020 ◽  
Vol 5 (12) ◽  
pp. e003622
Author(s):  
Iruka N Okeke ◽  
Nicholas Feasey ◽  
Julian Parkhill ◽  
Paul Turner ◽  
Direk Limmathurotsakul ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Low Income ◽  
Susceptibility Testing ◽  
Point Of Care ◽  
Nucleic Acid Amplification ◽  
High Tech ◽  
Surveillance Systems ◽  
Data Handling ◽  
Data Formats ◽  
Nucleic Acid Amplification Technology

The scope and trajectory of today’s escalating antimicrobial resistance (AMR) crisis is inadequately captured by existing surveillance systems, particularly those of lower income settings. AMR surveillance systems typically collate data from routine culture and susceptibility testing performed in diagnostic bacteriology laboratories to support healthcare. Limited access to high quality culture and susceptibility testing results in the dearth of AMR surveillance data, typical of many parts of the world where the infectious disease burden and antimicrobial need are high. Culture and susceptibility testing by traditional techniques is also slow, which limits its value in infection management. Here, we outline hurdles to effective resistance surveillance in many low-income settings and encourage an open attitude towards new and evolving technologies that, if adopted, could close resistance surveillance gaps. Emerging advancements in point-of-care testing, laboratory detection of resistance through or without culture, and in data handling, have the potential to generate resistance data from previously unrepresented locales while simultaneously supporting healthcare. Among them are microfluidic, nucleic acid amplification technology and next-generation sequencing approaches. Other low tech or as yet unidentified innovations could also rapidly accelerate AMR surveillance. Parallel advances in data handling further promise to significantly improve AMR surveillance, and new frameworks that can capture, collate and use alternate data formats may need to be developed. We outline the promise and limitations of such technologies, their potential to leapfrog surveillance over currently available, conventional technologies in use today and early steps that health systems could take towards preparing to adopt them.

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