scholarly journals In vivo characterisation of a catalase-based biosensor for real-time electrochemical monitoring of brain hydrogen peroxide in freely-moving animals

2017 ◽  
Vol 9 (8) ◽  
pp. 1253-1264 ◽  
Author(s):  
Saidhbhe L. O'Riordan ◽  
John P. Lowry
Keyword(s):  
Hydrogen Peroxide ◽  
Real Time ◽  
Freely Moving Rats ◽  
Freely Moving ◽  
Electrochemical Monitoring

A catalase-based microelectrochemical biosensor developed for real-time neurochemical monitoring of hydrogen peroxide (H2O2) was characterised in freely-moving rats.

Real-time, continuous electrochemical monitoring of drugs in vivo

2017 ◽  
Vol 171 ◽  
pp. e208
Author(s):  
Philip Vieira ◽  
Netzahualcóyotl Arroyo Currás ◽  
Jacob Somerson ◽  
Kyle Ploense ◽  
Kevin Plaxco ◽  
...  
Keyword(s):  
Real Time ◽  
Electrochemical Monitoring

In vivo study of tumor metabolism: an application of new multi-probe microdialysis system in the striatum of freely moving rats grafted with C6 cells

2000 ◽  
Vol 881 (2) ◽  
pp. 121-127 ◽  
Author(s):  
O Darbin ◽  
M Lonjon ◽  
M.H Quentien ◽  
J.F Michiels ◽  
P Grellier ◽  
...  
Keyword(s):  
Tumor Metabolism ◽  
In Vivo Study ◽  
C6 Cells ◽  
Freely Moving Rats ◽  
Freely Moving

scholarly journals In vivo Identification and Quantitative Evaluation of Carrier-Mediated Transport of Lactate at the Cellular Level in the Striatum of Conscious, Freely Moving Rats

1988 ◽  
Vol 8 (6) ◽  
pp. 848-856 ◽  
Author(s):  
Werner G. Kuhr ◽  
Cees J. van den Berg ◽  
Jakob Korf
Keyword(s):  
Cardiac Arrest ◽  
Electroconvulsive Shock ◽  
Time Course ◽  
Membrane Integrity ◽  
Extracellular Fluid ◽  
Direct Determination ◽  
Freely Moving Rats ◽  
Blood Brain ◽  
Freely Moving

Intracerebral dialysis has allowed the continuous, on-line measurement of lactate in the extracellular fluid (ECF) of conscious, freely moving rats. The rapid time response of the technique allows the direct determination of the time course of changes in lactate in ECF following externally imposed stimuli. The time course of lactate appearance in ECF was found to be considerably slower than that observed in tissue following electroconvulsive shock or during ischemia following cardiac arrest. The ECF data could be fit to an integrated Michaelis-Menten model that assumed reversible transport of lactate across the cell membrane. This transport was found to act only when energy supplies could maintain membrane integrity and function, since ECF levels of lactate failed to follow tissue levels after cardiac arrest when energy resources are depleted. The calculated rate of cellular lactate transport was two orders of magnitude faster than transport of lactate across the blood-brain barrier in the adult rat, and passive diffusion of lactate was not found to contribute significantly across either cell or blood-brain barriers. Probenecid, an inhibitor of acid transport, was able to block both the efflux of lactate from cell to ECF and the consequent reuptake of lactate by cells in the striatum of the rat following electroconvulsive shock or ischemia.

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