The detection of papaya ringspot virus coat protein using an electrochemical immunosensor

2016 ◽  
Vol 8 (48) ◽  
pp. 8534-8541 ◽  
Author(s):  
Rohini Bhat Valekunja ◽  
Vikramshankar Kamakoti ◽  
Anitha Peter ◽  
Shamprasad Phadnis ◽  
Shalini Prasad ◽  
...  
Keyword(s):  
Electrochemical Impedance Spectroscopy ◽  
Coat Protein ◽  
Impedance Spectroscopy ◽  
Plant Pathogen ◽  
Pathogen Detection ◽  
Electrochemical Impedance ◽  
Electrochemical Immunosensor ◽  
Papaya Ringspot Virus ◽  
Virus Coat ◽  
Plant Pathogen Detection

Electrochemical impedance spectroscopy-based immunosensors have enormous potential as a simple, rapid, economical, and field-deployable tool for plant pathogen detection.

scholarly journals Yeast Propagation Control: Low Frequency Electrochemical Impedance Spectroscopy as an Alternative for Cell Counting Chambers in Brewery Applications

Chemosensors ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 27
Author(s):  
Georg Christoph Brunauer ◽  
Oliver Spadiut ◽  
Alfred Gruber ◽  
Christoph Slouka
Keyword(s):  
Electrochemical Impedance Spectroscopy ◽  
Impedance Spectroscopy ◽  
Pathogen Detection ◽  
Electrochemical Impedance ◽  
Low Frequency ◽  
Charge Transfer Resistance ◽  
Cell Counting ◽  
Delayed Response ◽  
Transfer Resistance ◽  
Cell Counts

Electrochemical impedance spectroscopy is a powerful tool in life science for cell and pathogen detection, as well as for cell counting. The measurement principles and techniques using impedance spectroscopy are highly diverse. Differences can be found in used frequency range (β or α regime), analyzed quantities, like charge transfer resistance, dielectric permittivity of double layer capacitance and in off- or online usage. In recent contributions, applications of low-frequency impedance spectroscopy in the α regime were tested for determination of cell counts and metabolic burden in Escherichia coli and Saccharomyces cerevisiae. The established easy to use methods showed reasonable potential in the lab scale, especially for S. cerevisiae. However, until now, measurements for cell counts in food science are generally based on Thoma cell counting chambers. These microscopic cell counting methods decelerate an easy and quick prediction of yeast viability, as they are labor intensive and result in a time delayed response signal. In this contribution we tested our developed method using low frequency impedance spectroscopy locally at an industrial brewery propagation site and compared results to classic cell counting procedures.

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