A novel method to predict protein aggregations using two-dimensional native protein microfluidic chip electrophoresis

2016 ◽  
Vol 8 (47) ◽  
pp. 8306-8313 ◽  
Author(s):  
Shiyong Yu ◽  
Jiandong Xu ◽  
Kunjie Huang ◽  
Juan Chen ◽  
Jinyan Duan ◽  
...  
Keyword(s):  
Protein Aggregation ◽  
Microfluidic Chip ◽  
Protein Electrophoresis ◽  
Two Dimensional ◽  
Native Protein ◽  
Novel Method

A microfluidic chip native protein electrophoresis was established to predict protein aggregation.

Native Protein Separation by Isoelectric Focusing and Blue Gel Electrophoresis-Coupled Two-Dimensional Microfluidic Chip Electrophoresis

2014 ◽  
Vol 77 (19-20) ◽  
pp. 1339-1346 ◽  
Author(s):  
Ni Hou ◽  
Yu Chen ◽  
Shiyong Yu ◽  
Zongliang Quan ◽  
Chenhua Pan ◽  
...  
Keyword(s):  
Isoelectric Focusing ◽  
Microfluidic Chip ◽  
Gel Electrophoresis ◽  
Protein Separation ◽  
Two Dimensional ◽  
Native Protein

Novel method for calculating two-dimensional blade vortex interaction

AIAA Journal ◽  
1997 ◽  
Vol 35 ◽  
pp. 909-912
Author(s):  
Ronald J. Epstein ◽  
John A. Rule ◽  
Donald B. Bliss
Keyword(s):  
Two Dimensional ◽  
Vortex Interaction ◽  
Blade Vortex Interaction ◽  
Novel Method

scholarly journals Homogeneous 2D and 3D alignment of cardiomyocyte in dilated cardiomyopathy revealed by intravital heart imaging

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kiyoshi Masuyama ◽  
Tomoaki Higo ◽  
Jong-Kook Lee ◽  
Ryohei Matsuura ◽  
Ian Jones ◽  
...  
Keyword(s):  
Heart Failure ◽  
Dilated Cardiomyopathy ◽  
Cardiac Dysfunction ◽  
Three Dimensional ◽  
Single Layer ◽  
Specific Pattern ◽  
Two Dimensional ◽  
Heart Imaging ◽  
Novel Method ◽  
2D And 3D

AbstractIn contrast to hypertrophic cardiomyopathy, there has been reported no specific pattern of cardiomyocyte array in dilated cardiomyopathy (DCM), partially because lack of alignment assessment in a three-dimensional (3D) manner. Here we have established a novel method to evaluate cardiomyocyte alignment in 3D using intravital heart imaging and demonstrated homogeneous alignment in DCM mice. Whilst cardiomyocytes of control mice changed their alignment by every layer in 3D and position twistedly even in a single layer, termed myocyte twist, cardiomyocytes of DCM mice aligned homogeneously both in two-dimensional (2D) and in 3D and lost myocyte twist. Manipulation of cultured cardiomyocyte toward homogeneously aligned increased their contractility, suggesting that homogeneous alignment in DCM mice is due to a sort of alignment remodelling as a way to compensate cardiac dysfunction. Our findings provide the first intravital evidence of cardiomyocyte alignment and will bring new insights into understanding the mechanism of heart failure.

A novel method to fabricate two-dimensional nanomaterial based on electrospinning

2021 ◽  
Vol 143 ◽  
pp. 106275
Author(s):  
Wenjing Ji ◽  
Guojie Zhao ◽  
Cong Guo ◽  
Li Fan ◽  
Hua Deng ◽  
...  
Keyword(s):  
Two Dimensional ◽  
Novel Method

scholarly journals Specific and general stress proteins in Bacillus subtilis - a two-dimensional protein electrophoresis study

Microbiology ◽  
1997 ◽  
Vol 143 (3) ◽  
pp. 999-1017 ◽  
Author(s):  
J. Bernhardt ◽  
U. Volker ◽  
A. Volker ◽  
H. Antelmann ◽  
R. Schmid ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Stress Proteins ◽  
Protein Electrophoresis ◽  
Two Dimensional ◽  
General Stress

scholarly journals Enantioselective reaction monitoring utilizing two-dimensional heart-cut liquid chromatography on an integrated microfluidic chip

Lab on a Chip ◽  
2016 ◽  
Vol 16 (24) ◽  
pp. 4648-4652 ◽  
Author(s):  
Carsten Lotter ◽  
Elisabeth Poehler ◽  
Josef J. Heiland ◽  
Laura Mauritz ◽  
Detlev Belder
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Microfluidic Chip ◽  
High Performance ◽  
Reaction Monitoring ◽  
Two Dimensional ◽  
Flow Synthesis ◽  
Enantioselective Reaction ◽  
Micro Flow

Chip-integrated, two-dimensional high performance liquid chromatography is introduced to monitor enantioselective continuous micro-flow synthesis.

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