Optimization and validation of an environmentally friendly method for zinc determination in new and used lubricating oil samples

2016 ◽  
Vol 8 (48) ◽  
pp. 8435-8442 ◽  
Author(s):  
Mariana F. B. Mota ◽  
Ednilton M. Gama ◽  
Guilherme D. Rodrigues ◽  
Letícia M. Costa
Keyword(s):  
Environmentally Friendly ◽  
Lubricating Oil ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Used Lubricating Oil ◽  
Zinc Determination ◽  
First Time ◽  
Environmentally Friendly Method

This work presents the optimization and validation of a new environmentally friendly method for zinc quantification in new and used lubricating oil samples, using, for the first time, the extracting agent diphenylthiocarbazone (dithizone) associated with the aqueous two-phase system (ATPS).

scholarly journals A novel and environmentally friendly bioprocess for separation and partial purification of polysaccharides from Cordyceps sinensis mycelia by an aqueous two-phase system

RSC Advances ◽  
2017 ◽  
Vol 7 (60) ◽  
pp. 37659-37665 ◽  
Author(s):  
Zhigang Li ◽  
Ailin Chen ◽  
Zhencheng Li ◽  
Man Qu ◽  
Huayong Chen ◽  
...  
Keyword(s):  
High Efficiency ◽  
Environmentally Friendly ◽  
Cordyceps Sinensis ◽  
Partial Purification ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
System P ◽  
Active Substances

An aqueous two-phase system comprising ethanol and salt not only could purify CS polysaccharides with high efficiency, but also simultaneously separated multiple active substances through coupling with ultrafiltration.

scholarly journals Purification and Characterization of Lipase Produced by Leuconostoc mesenteroides Subsp. mesenteroides ATCC 8293 Using an Aqueous Two-Phase System (ATPS) Composed of Triton X-100 and Maltitol

Molecules ◽  
2018 ◽  
Vol 23 (7) ◽  
pp. 1800 ◽  
Author(s):  
Nurfadhilah Eko Sukohidayat ◽  
Mohammad Zarei ◽  
Badlishah Baharin ◽  
Mohd Manap
Keyword(s):  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Process Characteristics ◽  
Sds Page ◽  
Ph Range ◽  
Lipase Purification ◽  
Triton X ◽  
First Time

Purification of lipase produced by L. mesenteroides subsp. mesenteroides ATCC 8293 was conducted for the first time using a novel aqueous two-phase system (ATPS) composed of Triton X-100 and maltitol. The partitioning of lipase was optimized according to several parameters including pH, temperature, and crude load. Results showed that lipase preferentially migrated to the Triton X-100 rich phase and optimum lipase partitioning was achieved in ATPS at TLL of 46.4% and crude load of 20% at 30 °C and pH 8, resulting in high lipase purification factor of 17.28 and yield of 94.7%. The purified lipase showed a prominent band on SDS-PAGE with an estimated molecular weight of 50 kDa. The lipase was stable at the temperature range of 30–60 °C and pH range of 6–11, however, it revealed its optimum activity at the temperature of 37 °C and pH 8. Moreover, lipase exhibited enhanced activity in the presence of non-ionic surfactants with increased activity up to 40%. Furthermore, results exhibited that metals ions such as Na+, Mg2+, K+ and Ca2+ stimulated lipase activity. This study demonstrated that this novel system could be potentially used as an alternative to traditional ATPS for the purification and recovery of enzymes since the purified lipase still possesses good process characteristics after undergoing the purification process.

Phase Diagram of Aqueous Two-Phase System (ATPS) Composed of Polyethylene Glycol (PEG) and Gelatin

2012 ◽  
Vol 554-556 ◽  
pp. 286-294 ◽  
Author(s):  
Chao Ma ◽  
Xiao Dong Chen ◽  
Yin Qiu Kong ◽  
Li Ming Che
Keyword(s):  
Phase Diagram ◽  
Aqueous Solution ◽  
Phase Separation ◽  
Polyethylene Glycol ◽  
Solid Content ◽  
Phase System ◽  
Dense Phase ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
First Time

Phase diagram of aqueous two-phase system (ATPS) composed of polyethylene glycol (PEG) and gelatin is of paramount importance for the application of such system in microencapsulation of bioactive compounds. Phase separation of PEG/gelatin aqueous solution was investigated in the present work and the phase diagram of resultant ATPS was reported for the first time. The results show that phase separation will happen if the solid content of PEG/gelatin aqueous solution is higher than a critical value, resulting in an ATPS. The resultant ATPS consists of a low-density phase enriched in PEG and a dense phase enriched in gelatin. The phase compositions of the resultant ATPS were determined accurately using the method developed. The phase diagrams obtained show that higher solid content is required for the phase separation of PEG10,000/gelatin aqueous solution when compared with that of PEG20,000/gelatin one. And PEG is found to be more hydrophilic when compared with gelatin.

Enhancing the Sensitivity of DNA and Aptamer Probes in the Dextran/PEG Aqueous Two-Phase System

2021 ◽  
Author(s):  
Qiaoshu Chen ◽  
Yanwen Zhang ◽  
Hui Chen ◽  
Jianbo Liu ◽  
Juewen Liu
Keyword(s):  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

Continuous Purification of an Enveloped and Non-enveloped Viral Particle Using an Aqueous Two-Phase System

2021 ◽  
pp. 118753
Author(s):  
Dylan G. Turpeinen ◽  
Pratik U. Joshi ◽  
Seth A. Kriz ◽  
Supreet Kaur ◽  
Natalie M. Nold ◽  
...  
Keyword(s):  
Viral Particle ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

scholarly journals Partitioning of bovine lactoferrin in aqueous two-phase system containing poly(ethylene glycol) and sodium citrate

2015 ◽  
Vol 95 ◽  
pp. 118-124 ◽  
Author(s):  
Angélica Ribeiro da Costa ◽  
Jane Sélia dos Reis Coimbra ◽  
Luísa Alexandre Ferreira ◽  
João Carlos Marcos ◽  
Igor José Boggione Santos ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Sodium Citrate ◽  
Bovine Lactoferrin ◽  
Phase System ◽  
Poly Ethylene Glycol ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Poly Ethylene
Sign in / Sign up

Export Citation Format

Share Document