Development of a polychlorinated biphenyl screening method with 3 μL of blood

2016 ◽  
Vol 8 (31) ◽  
pp. 6030-6037
Author(s):  
Kazutoshi Nose ◽  
Hisatoshi Yabushita ◽  
Tetsuya Hirai ◽  
Tomiko Tachikawa
Keyword(s):  
Polychlorinated Biphenyls ◽  
Filter Paper ◽  
Whole Blood ◽  
Polychlorinated Biphenyl ◽  
Capillary Tube ◽  
Screening Method ◽  
Blood Samples ◽  
Whole Blood Samples

In the present study, we developed a method of screening for polychlorinated biphenyls (PCBs) by using 3 μL whole-blood samples accurately metered with a capillary tube on filter paper.

Non-Aqueous CE Screening Method for 14 Psychotropic Drugs in Whole Blood Samples

2007 ◽  
Vol 65 (5-6) ◽  
pp. 313-317 ◽  
Author(s):  
K. Madej ◽  
A. Marczyk ◽  
M. Woźniakiewicz
Keyword(s):  
Psychotropic Drugs ◽  
Whole Blood ◽  
Screening Method ◽  
Blood Samples ◽  
Whole Blood Samples

An Automated Procedure for Blood Phenylalanine

1965 ◽  
Vol 11 (5) ◽  
pp. 541-546 ◽  
Author(s):  
John B Hill ◽  
George K Summer ◽  
Murray W Pender ◽  
Norris O Roszel
Keyword(s):  
Filter Paper ◽  
Whole Blood ◽  
Blood Samples ◽  
Blood Phenylalanine ◽  
Large Populations ◽  
Fluorometric Analysis ◽  
Whole Blood Samples

Abstract The fluorometric analysis of phenylalanine described by McCaman and Robins has been adapted to automation. The procedure may be used on 20-µl. whole-blood samples which may be stored on filter paper for elution. The procedure is rapid, reproducible, and accurate, making it useful for screening large populations for phenylketonuria.

Comparative methodology for the detection and differentiation of circulating microfilariae of Dirofilaria immitis in the dog

2004 ◽  
Vol 78 (2) ◽  
pp. 137-140 ◽  
Author(s):  
M.E. Mylonakis ◽  
E. Papadopoulos ◽  
A.F. Koutinas ◽  
C. Paitaki ◽  
L. Leontides
Keyword(s):  
Whole Blood ◽  
Dirofilaria Immitis ◽  
Blood Smear ◽  
Capillary Tube ◽  
Buffy Coat ◽  
The Other ◽  
Blood Samples ◽  
Comparative Methodology ◽  
Whole Blood Samples ◽  
Knott’S Test

AbstractThe sensitivities of the Knott's test (four 20-μl sediment aliquots), quantitative buffy coat capillary tube method (QBC tube, 111 μl of whole blood) and direct blood smear (DBS, 20 μl of whole blood) were evaluated for the detection of microfilaraemia in dogs. Undiluted whole blood samples taken from 70 Dirofilaria immitis antigen-positive dogs and 10 serially diluted microfilaraemic blood samples at concentrations of 400, 200, 100, 50, 25 and 12 microfilariae (mff) ml−1 were examined. For filarial speciation, the buffy coat of QBC tubes was mixed with one drop of methylene blue–formalin solution and examined as a direct smear. In 52/70 microfilaraemic blood samples, the number of mff ranged from 12 to 321987 ml−1 (median: 3199 ml−1). The diagnostic sensitivity of the Knott's test, QBC tube method and DBS in undiluted blood samples attained the 100%, 98% and 92.3% levels, respectively. Eighteen dogs tested amicrofilaraemic by all three methods. At concentrations of 400 mff ml−1, a 100% sensitivity was found by all three methods, while at 200 mff ml−1 the Knott's test, QBC tube and DBS were 100%, 100% and 90% sensitive, respectively. The relevant figures at 100 mff ml−1 were 100%, 100% and 80%, at 50 mff ml−1 100%, 100% and 50%, at 25 mff ml−1 100%, 100% and 10% and at 12 mff ml−1 80%, 50% and 10%. At 50 and 25 mff ml−1, the DBS was less sensitive compared to the other two methods, while at 12 mff ml−1, only to the Knott's test. A significant correlation was found between the QBC tube method and Knott's test regarding mff speciation. Therefore, the QBC method may be considered a reliable alternative to the Knott's test for both the detection and speciation of mff in the dog.

Comprehensive Drug Screening of Whole Blood by LC–HRMS–MS in a Forensic Laboratory

2020 ◽  
Author(s):  
Jon B Stephenson ◽  
Melanie L Flater ◽  
Joseph Austin ◽  
Lisa T Bain ◽  
Lisa A Holt ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Drug Screening ◽  
Whole Blood ◽  
Drugs Of Abuse ◽  
Screening Method ◽  
Drug Analysis ◽  
Screening Methods ◽  
Blood Samples ◽  
Traditional Drug ◽  
Whole Blood Samples

Abstract As the number of prescriptions, over-the-counter medications and drugs of abuse continue to increase, forensic laboratories are faced with the challenge of developing more comprehensive screening methods in order to detect them in whole blood samples. Another challenge faced by forensic laboratories is detecting and identifying novel synthetic compounds as they emerge and change. Traditional drug screening methods include enzyme immunoassay (EIA) and either gas or liquid chromatography paired with mass spectrometry (GC–MS or LC–MS-MS, respectively). While these methods are good, they have their disadvantages. For example, EIA requires special reagents for each drug class, GC–MS requires extensive sample preparation, and LC–MS-MS only detects drugs on a known inclusion lists of compounds of interest. Described below is the development of a robust and comprehensive screening method for drugs in whole blood samples that eliminates the aforementioned disadvantages of the traditional methods. Using a Q Exactive Focus™ liquid chromatography–high-resolution accurate mass spectrometer (LC–HRMS-MS), a method was developed that is capable of detecting ~200 drugs at a concentration of 2 μg/L for most analytes. This method also employs a more automated data processing feature which reduces processing time. Finally, it has the added benefit of retroactive data analysis, which allows it to be used for unknown drug analysis as well. Used as an initial screening method, the comprehensive drug screen using LC–HRMS-MS has the potential to take on two of the most important challenges faced by forensic laboratories today.

Steadiness/stability of antiepileptic drugs in serum, plasma and whole-blood samples under various storage methods

2010 ◽  
Vol 41 (02) ◽  
Author(s):  
N Shazi ◽  
A Böss ◽  
HJ Merkel ◽  
F Scharbert ◽  
D Hannak ◽  
...  
Keyword(s):  
Antiepileptic Drugs ◽  
Whole Blood ◽  
Blood Samples ◽  
Storage Methods ◽  
Whole Blood Samples
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