Comparison of three chemiluminescence detection methods for growth hormone secretagogues competitive receptor assay in urine

2016 ◽  
Vol 8 (23) ◽  
pp. 4600-4607
Author(s):  
P. Ferro ◽  
R. Gutierrez-Gallego ◽  
J. Bosch ◽  
J. Segura
Keyword(s):  
Growth Hormone ◽  
Drug Testing ◽  
Detection Methods ◽  
Chemiluminescence Detection ◽  
Growth Hormone Secretagogues ◽  
Growth Hormone Secretagogue ◽  
Sports Drug Testing ◽  
Receptor Assay

The list of growth hormone secretagogue (GHS) products is growing steadily, which presents a challenge in sports drug testing.

scholarly journals Fit-for-Purpose Radio Receptor Assay for the Determination of Growth Hormone Secretagogues in Urine

2015 ◽  
Vol 20 (10) ◽  
pp. 1268-1276 ◽  
Author(s):  
P. Ferro ◽  
R. Gutiérrez-Gallego ◽  
J. Bosch ◽  
M. Farré ◽  
J. Segura
Keyword(s):  
Growth Hormone ◽  
Screening Method ◽  
Detection Methods ◽  
Growth Hormone Secretagogues ◽  
Receptor Assay ◽  
Depth Study ◽  
Fit For Purpose ◽  
Key Variables ◽  
Current Detection

The everlasting pharmacological development is continuously producing new substances with potential doping abuse. Among these, secretagogues are very prone to misuse by athletes for their properties to release growth hormone (GH) and some limitations in the actual analytical methods to detect them. In this paper, an in-depth study on the key variables of the radio receptor method previously developed by our group is performed and a fit-for-purpose protocol is established. Thus, this sensitive and robust screening method is proposed as an intelligent and preventive antidoping method to detect new growth hormone secretagogues (GHSs) in exceptional suspicious urine samples obtained from athletes and will support the current detection methods based on liquid chromatography–mass spectrometry (LC-MS).

Activation of growth hormone secretagogue receptor induces time-dependent clock phase delay in mice

2014 ◽  
Vol 307 (6) ◽  
pp. E515-E526 ◽  
Author(s):  
Lan Zhou ◽  
Qian Gao ◽  
Peng Zhang ◽  
Shu Guo ◽  
Jingli Gu ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Camp Response Element Binding ◽  
Phase Delay ◽  
Constant Darkness ◽  
Bolus Administration ◽  
Growth Hormone Secretagogues ◽  
Growth Hormone Secretagogue Receptor ◽  
Growth Hormone Secretagogue ◽  
Downstream Signaling ◽  
Ghs Receptor

Early studies have reported a phase-shifting effect of growth hormone secretagogues (GHSs). This study aimed to determine the mechanism of action of GHSs. We examined the response of the hypothalamic suprachiasmatic nuclei (SCN) to growth hormone releasing peptide-6 (GHRP-6) by assessing effects on the phase of locomotor activity rhythms, SCN neuronal discharges, and the potential signaling pathways involved in the drug action on circadian rhythms. The results showed that bolus administration of GHRP-6 (100 μg/kg ip) at the beginning of subjective night (CT12) induced a phase delay of the free-running rhythms in male C57BL/6J mice under constant darkness, but did not elicit phase shift at other checked circadian time (CT) points. The phase-delay effect of GHRP-6 was abolished by d-(+)-Lys-GHRP-6 (GHS receptor antagonist), KN-93 [calcium/calmodulin-dependent protein kinase II (CaMK) II inhibitor], or anti-phosphorylated (p)-cAMP response element-binding protein (CREB) antibody. Further analyses demonstrated that GHRP-6 at CT12 induced higher calcium mobilization and neuronal discharge in the SCN compared with that at CT6, decreased the levels of glutamate and γ-aminobutyric acid, increased the levels of p-CaMKII, p-CREB, and period 1, and delayed the circadian expressions of circadian locomotor output cycles kaput, Bmal1, and prokineticin 2 in the SCN; these signaling changes resulted in behavioral phase delay. Collectively, GHRP-6 induces a CT-dependent phase delay via activating GHS receptor and the downstream signaling, which is partially similar to the signaling cascade of light-induced phase delay at early night. These novel observations may help to better understand the role of GHSs in circadian physiology.

scholarly journals The synthesis of a small library of prospective growth hormone secretagogues

1999 ◽  
Vol 64 (10) ◽  
pp. 571-576
Author(s):  
Vojislav Pejovic ◽  
Vukic Soskic ◽  
Jelena Joksimovic
Keyword(s):  
Amino Acids ◽  
Growth Hormone ◽  
Combinatorial Chemistry ◽  
Methodological Approach ◽  
Rabbit Antiserum ◽  
Building Blocks ◽  
Growth Hormone Secretagogues ◽  
Growth Hormone Secretagogue ◽  
Design And Synthesis ◽  
Polyclonal Rabbit Antiserum

Employing tools of combinatorial chemistry, an original methodological approach has been developed and applied for the design and synthesis of a small library of peptide-like compounds, prospective growth hormone (GH) secretagogues. For this purpose seven building blocks of tBoc- and Fmoc-protected amino acids was used. In this way, a small, tripeptoid library on polyethylene glycolmonomethyl ether 5000 (PEG 5000) as a soluble support was obtained. The library was screened by a new, simple system, based on polyclonal rabbit antiserum raised against "GH secretagogue pharmacophore" of a known growth hormone secretagogue GHRP-6 (Hexarelin?) and the most promising GH secretagogue candidate was selected.

scholarly journals Growth hormone secretagogue receptor in dopamine neurons controls appetitive and consummatory behaviors towards high-fat diet in ad-libitum fed mice

2020 ◽  
Vol 119 ◽  
pp. 104718
Author(s):  
María Paula Cornejo ◽  
Franco Barrile ◽  
Daniela Cassano ◽  
Julieta Paola Aguggia ◽  
Guadalupe García Romero ◽  
...  
Keyword(s):  
Growth Hormone ◽  
High Fat Diet ◽  
Dopamine Neurons ◽  
High Fat ◽  
Growth Hormone Secretagogue Receptor ◽  
Growth Hormone Secretagogue ◽  
Ad Libitum

scholarly journals Paper spray mass spectrometry – A potential complementary technique for the detection of polar compounds in sports drug testing

2020 ◽  
Vol 12 (11-12) ◽  
pp. 1658-1665 ◽  
Author(s):  
Christian Görgens ◽  
Katherine Walker ◽  
Cornelia Boeser ◽  
Neloni Wijeratne ◽  
Claudia Martins ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Drug Testing ◽  
Polar Compounds ◽  
Paper Spray ◽  
Complementary Technique ◽  
Sports Drug Testing

Novel Isoxazole Carboxamides as Growth Hormone Secretagogue Receptor (GHS-R) Antagonists.

ChemInform ◽  
2005 ◽  
Vol 36 (4) ◽  
Author(s):  
Bo Liu ◽  
Gang Liu ◽  
Zhili Xin ◽  
Michael D. Serby ◽  
Hongyu Zhao ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Growth Hormone Secretagogue Receptor ◽  
Growth Hormone Secretagogue

Discovery, synthesis, and structure–activity studies of tetrazole based growth hormone secretagogues

2007 ◽  
Vol 17 (21) ◽  
pp. 5928-5933 ◽  
Author(s):  
Andrés S. Hernández ◽  
Peter T.W. Cheng ◽  
Christa M. Musial ◽  
Stephen G. Swartz ◽  
Rocco J. George ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Growth Hormone Secretagogues ◽  
Structure Activity
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