High performance liquid chromatography-quantitative nuclear magnetic resonance (HPLC-qNMR) with a two-signal suppression method for purity assessment of avermectin B1a

2016 ◽  
Vol 8 (22) ◽  
pp. 4482-4486 ◽  
Author(s):  
Ting Huang ◽  
Wei Zhang ◽  
Xinhua Dai ◽  
Na Li ◽  
Liang Huang ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
Magnetic Resonance ◽  
High Performance ◽  
Signal Suppression ◽  
Quantitative Nuclear Magnetic Resonance ◽  
Suppression Method ◽  
High Separation

HPLC-qNMR is a technology with great potential, which combines the high separation capability of HPLC and the high qualitative/quantitative analysis capability of NMR.

QUALITY ASSESSMENT AND QUANTIFICATION OF GENISTEIN IN DIETARY SUPPLEMENTS BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY, QUANTITATIVE NUCLEAR MAGNETIC RESONANCE, AND TWO-DIMENSIONAL DIFFUSION ORDERED SPECTROSCOPY 1H

2020 ◽  
pp. 132-142
Author(s):  
VLACHOU IOANNA ◽  
PAPASOTIRIOU IOANNIS
Keyword(s):  
Nuclear Magnetic Resonance ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Magnetic Resonance ◽  
Dietary Supplements ◽  
Active Ingredient ◽  
High Performance ◽  
Two Dimensional ◽  
Quantitative Nuclear Magnetic Resonance ◽  
Dosy Nmr

Objective: The aim of the present study is to analyze four herbal dietary supplements containing genistein by liquid chromatography, quantitative nuclear magnetic resonance (qNMR), and diffusion ordered spectroscopy (DOSY) 1H NMR. Methods: Quantification of the active ingredient, genistein, is carried out by high-performance liquid chromatography (HPLC) and qNMR. Two-dimensional (2D) DOSY NMR also allows the qualitative analysis of the samples with the detection of active ingredient and excipients present in the formulations. Results: The validated HPLC and qNMR methods showed that all four supplements contain genistein in different amounts, and 2D DOSY NMR provides a clear image of all ingredients in the formulations. Conclusion: The use of the three techniques provides detailed information on each product and its contents, and all of them are currently used for the quality control of natural supplements by our laboratory.

Determination and Identification of Chondroitin Sulfate from Tilapia Byproducts

2013 ◽  
Vol 690-693 ◽  
pp. 1318-1321 ◽  
Author(s):  
Zhen Hua Duan ◽  
Chang Yu Cheng ◽  
Yang Hai ◽  
Ju Lan Wang
Keyword(s):  
Nuclear Magnetic Resonance ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Magnetic Resonance ◽  
Chondroitin Sulfate ◽  
Infrared Spectra ◽  
Oreochromis Niloticus ◽  
High Performance ◽  
Nmr Spectra ◽  
Ir And Nmr Spectra

In order to probe the structure of chondroitin sulfate (CS) from tilapia fish, the CS was extracted from tilapia (Oreochromis niloticus) byproducts with the combination of ultrasonic and microwave, some means including high performance liquid chromatography (HPLC), infrared spectra (IR) and nuclear magnetic resonance (NMR) were used in this paper. The data of HPLC exhibited that the obtained chondroitin sulfate is of highly purity (98.78%). IR and NMR spectra indicated that the sample consisted of both chondroitin-4-sulfate and chondroitin-6-sulfate, and the proportion of chondroitin-6-sulfate was higher than chondroitin-4-sulfate.

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