The evaluation of matrix effects in pesticide multi-residue methods via matrix fingerprinting using liquid chromatography electrospray high-resolution mass spectrometry

2016 ◽  
Vol 8 (23) ◽  
pp. 4664-4673 ◽  
Author(s):  
María del Mar Gómez-Ramos ◽  
Łukasz Rajski ◽  
Ana Lozano ◽  
Amadeo R. Fernández-Alba
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
High Resolution ◽  
Matrix Effects ◽  
High Resolution Mass Spectrometry ◽  
High Resolution Mass ◽  
P Matrix ◽  
Resolution Mass

Matrix fingerprinting of twenty-three different commodities was evaluated.

scholarly journals Detection of Methylphenidate in Equine Hair Using Liquid Chromatography–High-Resolution Mass Spectrometry

Molecules ◽  
2021 ◽  
Vol 26 (19) ◽  
pp. 5798
Author(s):  
Benjamin C. Moeller ◽  
Luis Flores ◽  
Amel Clifford ◽  
Gwendolyne Alarcio ◽  
Mary Mosburg ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
High Resolution ◽  
Matrix Effects ◽  
High Resolution Mass Spectrometry ◽  
Horse Racing ◽  
High Resolution Mass ◽  
Accuracy And Precision ◽  
Product Ions ◽  
Resolution Mass

Methylphenidate is a powerful central nervous system stimulant with a high potential for abuse in horse racing. The detection of methylphenidate use is of interest to horse racing authorities for both prior to and during competition. The use of hair as an alternative sampling matrix for equine anti-doping has increased as the number of detectable compounds has expanded. Our laboratory developed a liquid chromatography–high-resolution mass spectrometry method to detect the presence of methylphenidate in submitted samples. Briefly, hair was decontaminated, cut, and pulverized prior to liquid–liquid extraction in basic conditions before introduction to the LC-MS system. Instrumental analysis was conducted using a Thermo Q Exactive mass spectrometer using parallel reaction monitoring using a stepped collision energy to obtain sufficient product ions for qualitative identification. The method was validated and limits of quantitation, linearity, matrix effects, recovery, accuracy, and precision were determined. The method has been applied to confirm the presence of methylphenidate in official samples submitted by racing authorities.

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