Simultaneous quantification of six sesquiterpene lactones and a flavonoid in the whole life stage of Inula salsoloides by high performance liquid chromatography

2016 ◽  
Vol 8 (17) ◽  
pp. 3587-3591 ◽  
Author(s):  
Lu Bai ◽  
Mi Jiang ◽  
Sen Guo ◽  
Qingchao Liu ◽  
Xinxin Zhang ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Herbal Medicine ◽  
Chinese Herbal Medicine ◽  
High Performance ◽  
Sesquiterpene Lactones ◽  
Life Stage ◽  
Bioactive Substances ◽  
Simultaneous Quantification ◽  
Chinese Herbal

Sesquiterpene lactones and flavonoids are the most bioactive substances in Chinese herbal medicine Inula salsoloides.

Study on Multiple Pesticides by High Performance Liquid Chromatography-Mass Spectrometry Using Modified QuEChERS Method - a Case Study on CHM

2011 ◽  
Vol 361-363 ◽  
pp. 1752-1755
Author(s):  
Yi Wei Dong ◽  
Wei Bai ◽  
Yu Zhong Li ◽  
Chun Ying Xu ◽  
Fu Li Fang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Herbal Medicine ◽  
Chinese Herbal Medicine ◽  
High Performance ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chinese Herbal ◽  
Chromatography Mass Spectrometry ◽  
Wide Range

QuEChERS is an acetonitrile partitioning and dispersive solid-phase extraction (D-SPE) method that widely used in the analysis of multiple classes of pesticide residues in vegetable and fruit. In this paper, we modified it to fulfill analysis on much more complex matrix-Chinese herbal medicine, and High Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS) was chosen for analysis. This method could be applied to quantitative and qualitative analysis of a wide range of pesticides in Chinese herbal medicine (Fructus Aurantii Immaturus as sample).

scholarly journals Chemical Profiles and Simultaneous Quantification of Aurantii fructus by Use of HPLC-Q-TOF-MS Combined with GC-MS and HPLC Methods

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2189 ◽  
Author(s):  
Yingjie He ◽  
Zongkai Li ◽  
Wei Wang ◽  
Suren Sooranna ◽  
Yiting Shi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quality Control ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Gradient Elution ◽  
Chemical Components ◽  
Bioactive Substances ◽  
Flight Mass Spectrometry ◽  
Tof Ms

Aurantii fructus (AF) is a traditional Chinese medicine that has been used to improve gastrointestinal motility disorders for over a thousand years, but there is no exhaustive identification of the basic chemical components and comprehensive quality control of this herb. In this study, high-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry (HPLC-Q-TOF-MS) and gas chromatography coupled mass spectrometry (GC-MS) were employed to identify the basic chemical compounds, and high-performance liquid chromatography (HPLC) was developed to determine the major biochemical markers from AF extract. There were 104 compounds belonging to eight structure types, including 13 amino acids or peptides, seven alkaloids, 18 flavanones, 14 flavones, 15 polymethoxyflavonoids, six triterpenoids, nine coumarins, and 18 volatile oils, as well as four other compounds that were systematically identified as the basic components from AF, and among them, 41 compounds were reported for the first time. Twelve bioactive ingredients were chosen as the benchmark markers to evaluate the quality of AF. The analysis was completed with a gradient elution at a flow rate of 0.7 mL/min within 55 min. This efficient method was validated showing good linearity, precision, stability, repeatability and recovery. Furthermore, the method was successfully applied to the simultaneous determination of 12 chemical markers in different samples of AF. This study could be applied to the identification of multiple bioactive substances and improve the quality control of AF.

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