scholarly journals A disposable chemical heater and dry enzyme preparation for lysis and extraction of DNA and RNA from microorganisms

2016 ◽  
Vol 8 (14) ◽  
pp. 2880-2886 ◽  
Author(s):  
J. R. Buser ◽  
X. Zhang ◽  
S. A. Byrnes ◽  
P. D. Ladd ◽  
E. K. Heiniger ◽  
...  
Keyword(s):  
Enzyme Preparation ◽  
Point Of Care ◽  
Molecular Diagnostic ◽  
Dna And Rna ◽  
Sample Heating

A simple dry enzyme and sample heating approach for point-of-care molecular diagnostic devices is demonstrated.

Molecular diagnostic of toxigenic Corynebacterium diphtheriae strain by DNA sensor potentially suitable for electrochemical point-of-care diagnostic

Talanta ◽  
2021 ◽  
Vol 227 ◽  
pp. 122161
Author(s):  
Kasper Marchlewicz ◽  
Iga Ostrowska ◽  
Sławomir Oszwałdowski ◽  
Aleksandra Zasada ◽  
Robert Ziółkowski ◽  
...  
Keyword(s):  
Point Of Care ◽  
Corynebacterium Diphtheriae ◽  
Molecular Diagnostic ◽  
Dna Sensor

scholarly journals Modular development of a prototype point of care molecular diagnostic platform for sexually transmitted infections

2016 ◽  
Vol 38 (8) ◽  
pp. 741-748 ◽  
Author(s):  
Manoharanehru Branavan ◽  
Ruth E. Mackay ◽  
Pascal Craw ◽  
Angel Naveenathayalan ◽  
Jeremy C. Ahern ◽  
...  
Keyword(s):  
Sexually Transmitted Infections ◽  
Point Of Care ◽  
Molecular Diagnostic ◽  
Sexually Transmitted

Point-of-care molecular diagnostic devices: an overview

2015 ◽  
Vol 16 (12) ◽  
pp. 1399-1409 ◽  
Author(s):  
Chi Lan Nguyen Vu ◽  
Jianxiong Chan ◽  
Marian Todaro ◽  
Stan Skafidas ◽  
Patrick Kwan
Keyword(s):  
Point Of Care ◽  
Molecular Diagnostic

A point-of-care diagnostic for differentiating Ebola from endemic febrile diseases

2018 ◽  
Vol 10 (471) ◽  
pp. eaat0944 ◽  
Author(s):  
David Sebba ◽  
Alexander G. Lastovich ◽  
Melody Kuroda ◽  
Eric Fallows ◽  
Joshua Johnson ◽  
...  
Keyword(s):  
Ebola Virus ◽  
Clinical Symptoms ◽  
Point Of Care ◽  
Hemorrhagic Fever ◽  
Diagnostic Methods ◽  
Outbreak Detection ◽  
Molecular Diagnostic ◽  
Clinical Samples ◽  
Live Virus ◽  
And Control

Hemorrhagic fever outbreaks such as Ebola are difficult to detect and control because of the lack of low-cost, easily deployable diagnostics and because initial clinical symptoms mimic other endemic diseases such as malaria. Current molecular diagnostic methods such as polymerase chain reaction require trained personnel and laboratory infrastructure, hindering diagnostics at the point of need. Although rapid tests such as lateral flow can be broadly deployed, they are typically not well-suited for differentiating among multiple diseases presenting with similar symptoms. Early detection and control of Ebola outbreaks require simple, easy-to-use assays that can detect and differentiate infection with Ebola virus from other more common febrile diseases. Here, we developed and tested an immunoassay technology that uses surface-enhanced Raman scattering (SERS) tags to simultaneously detect antigens from Ebola, Lassa, and malaria within a single blood sample. Results are provided in <30 min for individual or batched samples. Using 190 clinical samples collected from the 2014 West African Ebola outbreak, along with 163 malaria positives and 233 negative controls, we demonstrated Ebola detection with 90.0% sensitivity and 97.9% specificity and malaria detection with 100.0% sensitivity and 99.6% specificity. These results, along with corresponding live virus and nonhuman primate testing of an Ebola, Lassa, and malaria 3-plex assay, indicate the potential of the SERS technology as an important tool for outbreak detection and clinical triage in low-resource settings.

scholarly journals Innovative point-of-care molecular diagnostic test for COVID-19 in India

2020 ◽  
Vol 1 (7) ◽  
pp. e277
Author(s):  
Nivedita Gupta ◽  
Salaj Rana ◽  
Harpreet Singh
Keyword(s):  
Diagnostic Test ◽  
Point Of Care ◽  
Molecular Diagnostic ◽  
Molecular Diagnostic Test

scholarly journals Enhancing colorimetric loop-mediated isothermal amplification speed and sensitivity with guanidine chloride

BioTechniques ◽  
2020 ◽  
Vol 69 (3) ◽  
pp. 178-185 ◽  
Author(s):  
Yinhua Zhang ◽  
Guoping Ren ◽  
Jackson Buss ◽  
Andrew J Barry ◽  
Gregory C Patton ◽  
...  
Keyword(s):  
Guanidine Hydrochloride ◽  
Isothermal Amplification ◽  
Diagnostic Methods ◽  
Molecular Diagnostic ◽  
Simple Method ◽  
Diagnostic Assays ◽  
Loop Mediated Isothermal Amplification ◽  
Dna And Rna ◽  
Versatile Technique ◽  
Primer Sets

Loop-mediated isothermal amplification (LAMP) is a versatile technique for detection of target DNA and RNA, enabling rapid molecular diagnostic assays with minimal equipment. The global SARS-CoV-2 pandemic has presented an urgent need for new and better diagnostic methods, with colorimetric LAMP utilized in numerous studies for SARS-CoV-2 detection. However, the sensitivity of colorimetric LAMP in early reports has been below that of the standard RT-qPCR tests, and we sought to improve performance. Here we report the use of guanidine hydrochloride and combined primer sets to increase speed and sensitivity in colorimetric LAMP, bringing this simple method up to the standards of sophisticated techniques and enabling accurate, high-throughput diagnostics.

scholarly journals Smart cup: A minimally-instrumented, smartphone-based point-of-care molecular diagnostic device

2016 ◽  
Vol 229 ◽  
pp. 232-238 ◽  
Author(s):  
Shih-Chuan Liao ◽  
Jing Peng ◽  
Michael G. Mauk ◽  
Sita Awasthi ◽  
Jinzhao Song ◽  
...  
Keyword(s):  
Point Of Care ◽  
Molecular Diagnostic ◽  
Diagnostic Device

scholarly journals Multiplex logic processing isothermal diagnostic assays for an evolving virus

2018 ◽  
Author(s):  
Sanchita Bhadra ◽  
Miguel A. Saldaña ◽  
Hannah Grace Han ◽  
Grant L. Hughes ◽  
Andrew D. Ellington
Keyword(s):  
Point Of Care ◽  
Direct Analysis ◽  
Human Saliva ◽  
Ease Of Use ◽  
Molecular Diagnostic ◽  
One Pot ◽  
Diagnostic Assays ◽  
Specificity And Sensitivity ◽  
Isothermal Assay ◽  
Fail Safe

AbstractWe have developed a generalizable ‘smart molecular diagnostic’ capable of accurate point-of-care (POC) detection of variable nucleic acid targets. Our one-pot isothermal assay relies on multiplex execution of four loop-mediated isothermal amplification reactions, with primers that are degenerate and redundant, thereby increasing the breadth of targets while reducing the probability of amplification failure. An easy-to-read visual answer is computed directly by a multi-input Boolean OR gate signal transducer that uses degenerate strand exchange probes to assess any combination of amplicons. We demonstrate our platform by using the same assay to detect divergent Asian and African lineages of the evolving Zika virus (ZIKV), while maintaining selectivity against non-target viruses. Direct analysis of biological specimens proved possible, with 20 virions / µl being directly detected in human saliva within 90 minutes, and crudely macerated ZIKV-infected Aedes aegypti mosquitoes being identified with 100% specificity and sensitivity. The ease-of-use with minimal instrumentation, broad programmability, and built-in fail-safe reliability make our smart molecular diagnostic attractive for POC use.

scholarly journals System Architecture for IIoT-Based POC Molecular Diagnostic Device

2021 ◽  
Vol 6 (1) ◽  
pp. 60
Author(s):  
Byeong-Heon Kil ◽  
Ji-Seong Park ◽  
Chan-Young Park ◽  
Yu-Seop Kim ◽  
Jong-Dae Kim
Keyword(s):  
Point Of Care ◽  
Low Cost ◽  
Diagnostic System ◽  
Pcr Amplification ◽  
Dna Amplification ◽  
System Structure ◽  
World Health ◽  
Molecular Diagnostic ◽  
Target System ◽  
Open Platform

In this paper, we investigate an efficient structure for a point-of-care (POC) molecular diagnostic system based on the industrial Internet of things (IIoT). The target system can perform automated molecular diagnosis including DNA extraction, PCR amplification, and fluorescence detection. Samples and reagents are placed in a multi-room cartridge and loaded into the system. A rotating motor and a syringe motor control the cartridge to extract DNA from the sample. The extracted DNA is transferred to a polymerase chain reaction (PCR) chamber for DNA amplification and detection. The proposed system provides multiplexing of up to four colors. For POC molecular diagnostics, the World Health Organization demands features such as low volume, low cost, fast results, and a user-friendly interface. In this paper, we propose a system structure that can satisfy these requirements by using a PCR chip and open platform. A distributed structure is adopted for the convenience of maintenance, and a web-based GUI is adopted for the user’s convenience. We also investigated communication problems that may occur between system components. Using the proposed structure, the user can conveniently control from standard computing devices including a smartphone.

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