Assessment of coulometric array electrochemical detection coupled with HPLC-UV for the absolute quantitation of pharmaceuticals

The Analyst ◽  
2017 ◽  
Vol 142 (3) ◽  
pp. 525-536 ◽  
Author(s):  
Michael B. Hicks ◽  
Leah Salituro ◽  
Ian Mangion ◽  
Wes Schafer ◽  
Rong Xiang ◽  
...  
Keyword(s):  
Electrochemical Detection ◽  
Array Detector ◽  
Absolute Quantitation ◽  
Faraday’S Law ◽  
Novel Approach ◽  
Flow Through ◽  
Electrochemical Redox ◽  
The Absolute ◽  
Redox Conversion

A novel approach for the absolute quantitation of pharmaceuticals without standards using a flow-through coulometric array detector and the known electrochemical redox conversion applying Faraday's law,Q=N/Fn.

On-chip electrochemical detection of CdS quantum dots using normal and multiple recycling flow through modes

Lab on a Chip ◽  
2012 ◽  
Vol 12 (11) ◽  
pp. 2000 ◽  
Author(s):  
Mariana Medina-Sánchez ◽  
Sandrine Miserere ◽  
Sergio Marín ◽  
Gemma Aragay ◽  
Arben Merkoçi
Keyword(s):  
Quantum Dots ◽  
Electrochemical Detection ◽  
Cds Quantum Dots ◽  
Flow Through ◽  
On Chip ◽  
Multiple Recycling

scholarly journals Fabrication of Carbohydrate Chips Based on Polydopamine for Real-Time Determination of Carbohydrate–Lectin Interactions by QCM Biosensor

Polymers ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 1275 ◽  
Author(s):  
Kun Shang ◽  
Siyu Song ◽  
Yaping Cheng ◽  
Lili Guo ◽  
Yuxin Pei ◽  
...  
Keyword(s):  
Real Time ◽  
Con A ◽  
Quartz Crystals ◽  
Ulex Europaeus ◽  
Novel Approach ◽  
Ulex Europaeus Agglutinin I ◽  
Flow Through ◽  
Time Determination ◽  
Kinetics Of

A novel approach for preparing carbohydrate chips based on polydopamine (PDA) surface to study carbohydrate–lectin interactions by quartz crystal microbalance (QCM) biosensor instrument has been developed. The amino-carbohydrates were immobilized on PDA-coated quartz crystals via Schiff base reaction and/or Michael addition reaction. The resulting carbohydrate-chips were applied to QCM biosensor instrument with flow-through system for real-time detection of lectin–carbohydrate interactions. A series of plant lectins, including wheat germ agglutinin (WGA), concanavalin A (Con A), Ulex europaeus agglutinin I (UEA-I), soybean agglutinin (SBA), and peanut agglutinin (PNA), were evaluated for the binding to different kinds of carbohydrate chips. Clearly, the results show that the predicted lectin selectively binds to the carbohydrates, which demonstrates the applicability of the approach. Furthermore, the kinetics of the interactions between Con A and mannose, WGA and N-Acetylglucosamine were studied, respectively. This study provides an efficient approach to preparing carbohydrate chips based on PDA for the lectin–carbohydrate interactions study.

scholarly journals A novel approach to Verify air gap and SSD for proton radiotherapy using surface imaging

2019 ◽  
Vol 14 (1) ◽  
Author(s):  
Xiao Wang ◽  
Chi Ma ◽  
Rihan Davis ◽  
Rahul R. Parikh ◽  
Salma K. Jabbour ◽  
...  
Keyword(s):  
Body Surface ◽  
Imaging System ◽  
Air Gap ◽  
Surface Imaging ◽  
Proton Radiotherapy ◽  
Optical Surface ◽  
Air Gaps ◽  
Novel Approach ◽  
Proton Treatment ◽  
The Absolute

Abstract Purpose To develop a novel approach to accurately verify patient set up in proton radiotherapy, especially for the verification of the nozzle – body surface air gap and source-to-skin distance (SSD), the consistency and accuracy of which is extremely important in proton treatment. Methods Patient body surfaces can be captured and monitored with the optical surface imaging system during radiation treatment for improved intrafraction accuracy. An in-house software package was developed to reconstruct the patient body surface in the treatment position from the optical surface imaging reference capture and to calculate the corresponding nozzle – body surface air gap and SSD. To validate this method, a mannequin was scanned on a CT simulator and proton plans were generated for a Mevion S250 Proton machine with 20 gantry/couch angle combinations, as well as two different snout sizes, in the Varian Eclipse Treatment Planning Systems (TPS). The surface generated in the TPS from the CT scan was imported into the optical imaging system as an RT Structure for the purpose of validating and establishing a benchmark for ground truth comparison. The optical imaging surface reference capture was acquired at the treatment setup position after orthogonal kV imaging to confirm the positioning. The air gaps and SSDs calculated with the developed method from the surface captured at the treatment setup position (VRT surface) and the CT based surface imported from the TPS were compared to those calculated in TPS. The same approach was also applied to 14 clinical treatment fields for 10 patients to further validate the methodology. Results The air gaps and SSDs calculated from our program agreed well with the corresponding values derived from the TPS. For the phantom results, using the CT surface, the absolute differences in the air gap were 0.45 mm ± 0.33 mm for the small snout, and 0.51 mm ± 0.49 mm for the large snout, and the absolute differences in SSD were 0.68 mm ± 0.42 mm regardless of snout size. Using the VRT surface, the absolute differences in air gap were 1.17 mm ± 1.17 mm and 2.1 mm ± 3.09 mm for the small and large snouts, respectively, and the absolute differences in SSD were 0.81 mm ± 0.45 mm. Similarly, for patient data, using the CT surface, the absolute differences in air gap were 0.42 mm ± 0.49 mm, and the absolute differences in SSD were 1.92 mm ± 1.4 mm. Using the VRT surface, the absolute differences in the air gap were 2.35 mm ± 2.3 mm, and the absolute differences in SSD were 2.7 mm ± 2.17 mm. Conclusion These results showed the feasibility and robustness of using an optical surface imaging approach to conveniently determine the air gap and SSD in proton treatment, providing an accurate and efficient way to confirm the target depth at treatment.

scholarly journals A new approach for flow-through respirometry measurements in humans

2010 ◽  
Vol 298 (6) ◽  
pp. R1571-R1579 ◽  
Author(s):  
Edward L. Melanson ◽  
Jan P. Ingebrigtsen ◽  
Audrey Bergouignan ◽  
Kazunori Ohkawara ◽  
Wendy M. Kohrt ◽  
...  
Keyword(s):  
Water Vapor ◽  
Relative Humidity ◽  
Gas Exchange ◽  
Flow Rate ◽  
Water Vapor Pressure ◽  
Propane Combustion ◽  
Pull System ◽  
Novel Approach ◽  
Flow Through ◽  
Combustion Tests

Indirect whole room calorimetry is commonly used in studies of human metabolism. These calorimeters can be configured as either push or pull systems. A major obstacle to accurately calculating gas exchange rates in a pull system is that the excurrent flow rate is increased above the incurrent flow rate, because the organism produces water vapor, which also dilutes the concentrations of respiratory gasses in the excurrent sample. A common approach to this problem is to dry the excurrent gasses prior to measurement, but if drying is incomplete, large errors in the calculated oxygen consumption will result. The other major potential source of error is fluctuations in the concentration of O2 and CO2 in the incurrent airstream. We describe a novel approach to measuring gas exchange using a pull-type whole room indirect calorimeter. Relative humidity and temperature of the incurrent and excurrent airstreams are measured continuously using high-precision, relative humidity and temperature sensors, permitting accurate measurement of water vapor pressure. The excurrent flow rates are then adjusted to eliminate the flow contribution from water vapor, and respiratory gas concentrations are adjusted to eliminate the effect of water vapor dilution. In addition, a novel switching approach is used that permits constant, uninterrupted measurement of the excurrent airstream while allowing frequent measurements of the incurrent airstream. To demonstrate the accuracy of this approach, we present the results of validation trials compared with our existing system and metabolic carts, as well as the results of standard propane combustion tests.

scholarly journals A Novel Pretreatment-Free Duplex Chamber Digital PCR Detection System for the Absolute Quantitation of GMO Samples

2016 ◽  
Vol 17 (3) ◽  
pp. 402 ◽  
Author(s):  
Pengyu Zhu ◽  
Chenguang Wang ◽  
Kunlun Huang ◽  
Yunbo Luo ◽  
Wentao Xu
Keyword(s):  
Detection System ◽  
Digital Pcr ◽  
Pcr Detection ◽  
Absolute Quantitation ◽  
The Absolute

scholarly journals Note on the Standard of Relative Viscosity, and on “Negative Viscosity.”

1906 ◽  
Vol 25 (1) ◽  
pp. 227-230 ◽  
Author(s):  
W. W. Taylor
Keyword(s):  
Capillary Tube ◽  
Relative Viscosity ◽  
Absolute Viscosity ◽  
Flow Through ◽  
The Absolute ◽  
Negative Viscosity ◽  
Image Position

The absolute viscosity calculated from the formula(where p = the pressure, t the time, r the radius, l the length of capillary, and v the volume of liquid), which connects the viscosity of a liquid with the rate of flow through a long capillary tube, is not often made use of, mainly on account of the difficulty of accurately determining some of the constants (r in particular).

A label-free method based on MALDI-TOF mass spectrometry for the absolute quantitation of troponin T in mouse cardiac tissue

2008 ◽  
Vol 391 (5) ◽  
pp. 1969-1976 ◽  
Author(s):  
Mariano Bizzarri ◽  
Chiara Cavaliere ◽  
Patrizia Foglia ◽  
Chiara Guarino ◽  
Roberto Samperi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cardiac Tissue ◽  
Troponin T ◽  
Label Free ◽  
Absolute Quantitation ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
The Absolute
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