scholarly journals Detection of evoked acetylcholine release in mouse brain slices

The Analyst ◽  
2016 ◽  
Vol 141 (23) ◽  
pp. 6416-6421 ◽  
Author(s):  
R. Asri ◽  
B. O'Neill ◽  
J. C. Patel ◽  
K. A. Siletti ◽  
M. E. Rice
Keyword(s):  
Cyclic Voltammetry ◽  
Carbon Fiber ◽  
Mouse Brain ◽  
Neurotransmitter Release ◽  
Acetylcholine Release ◽  
Brain Slices ◽  
Fast Scan Cyclic Voltammetry ◽  
Carbon Fiber Microelectrode ◽  
The Brain

The study of transmitter interactions in the brain requires methodology to detect stimulus-driven neurotransmitter release. This report introduces an enzyme-coated 7 μm carbon-fiber microelectrode used with fast-scan cyclic voltammetry to detect evoked acetylcholine release in mouse brain slices.

scholarly journals Enzyme-Modified Carbon-Fiber Microelectrode for the Quantification of Dynamic Fluctuations of Nonelectroactive Analytes Using Fast-Scan Cyclic Voltammetry

2013 ◽  
Vol 85 (18) ◽  
pp. 8780-8786 ◽  
Author(s):  
Leyda Z. Lugo-Morales ◽  
Philip L. Loziuk ◽  
Amanda K. Corder ◽  
J. Vincent Toups ◽  
James G. Roberts ◽  
...  
Keyword(s):  
Cyclic Voltammetry ◽  
Carbon Fiber ◽  
Fast Scan Cyclic Voltammetry ◽  
Carbon Fiber Microelectrode

Wireless transmission of fast-scan cyclic voltammetry at a carbon-fiber microelectrode: proof of principle

2004 ◽  
Vol 140 (1-2) ◽  
pp. 103-115 ◽  
Author(s):  
Paul A. Garris ◽  
Robert Ensman ◽  
John Poehlman ◽  
Andy Alexander ◽  
Paul E. Langley ◽  
...  
Keyword(s):  
Cyclic Voltammetry ◽  
Carbon Fiber ◽  
Wireless Transmission ◽  
Fast Scan Cyclic Voltammetry ◽  
Carbon Fiber Microelectrode ◽  
Proof Of Principle

scholarly journals Real-Time Fast Scan Cyclic Voltammetry Detection and Quantification of Exogenously Administered Melatonin in Mice Brain

2020 ◽  
Vol 8 ◽  
Author(s):  
Elisa Castagnola ◽  
Elaine M. Robbins ◽  
Kevin M. Woeppel ◽  
Moriah McGuier ◽  
Asiyeh Golabchi ◽  
...  
Keyword(s):  
Cyclic Voltammetry ◽  
Real Time ◽  
Mouse Brain ◽  
Activated Carbon Fiber ◽  
Carbon Surface ◽  
Fast Scan Cyclic Voltammetry ◽  
Brain Functions ◽  
The Brain

Melatonin (MT) has been recently considered an excellent candidate for the treatment of sleep disorders, neural injuries, and neurological diseases. To better investigate the actions of MT in various brain functions, real-time detection of MT concentrations in specific brain regions is much desired. Previously, we have demonstrated detection of exogenously administered MT in anesthetized mouse brain using square wave voltammetry (SWV). Here, for the first time, we show successful detection of exogenous MT in the brain using fast scan cyclic voltammetry (FSCV) on electrochemically pre-activated carbon fiber microelectrodes (CFEs). In vitro evaluation showed the highest sensitivity (28.1 nA/μM) and lowest detection limit (20.2 ± 4.8 nM) ever reported for MT detection at carbon surface. Additionally, an extensive CFE stability and fouling assessment demonstrated that a prolonged CFE pre-conditioning stabilizes the background, in vitro and in vivo, and provides consistent CFE sensitivity over time even in the presence of a high MT concentration. Finally, the stable in vivo background, with minimized CFE fouling, allows us to achieve a drift-free FSCV detection of exogenous administered MT in mouse brain over a period of 3 min, which is significantly longer than the duration limit (usually < 90 s) for traditional in vivo FSCV acquisition. The MT concentration and dynamics measured by FSCV are in good agreement with SWV, while microdialysis further validated the concentration range. These results demonstrated reliable MT detection using FSCV that has the potential to monitor MT in the brain over long periods of time.

Carbon Fiber Multielectrode Array (CFMEA) for Multiplexing Neurochemical Measurements with Fast Scan Cyclic Voltammetry

2021 ◽  
Vol MA2021-02 (55) ◽  
pp. 1606-1606
Author(s):  
Alexander George Zestos ◽  
Favian Alberto Liu ◽  
Thomas Asrat ◽  
Harmain Rafi
Keyword(s):  
Cyclic Voltammetry ◽  
Carbon Fiber ◽  
Multielectrode Array ◽  
Fast Scan Cyclic Voltammetry

scholarly journals Laser Sharpening of Carbon Fiber Microelectrode Arrays for Brain Recording

2020 ◽  
Vol 8 (4) ◽  
Author(s):  
Tianshu Dong ◽  
Lei Chen ◽  
Albert Shih
Keyword(s):  
Carbon Fiber ◽  
Electrical Discharge Machining ◽  
Electrochemical Impedance ◽  
Microelectrode Arrays ◽  
Small Diameter ◽  
Input Voltage ◽  
Bath Surface ◽  
Insertion Force ◽  
Carbon Fiber Microelectrode ◽  
The Brain

Abstract Microwire microelectrode arrays (MEAs) are implanted in the brain for recording neuron activities to study the brain function. Among various microwire materials, carbon fiber stands out due to its small diameter (5–10 μm), relatively high Young's modulus, and low electrical resistance. Microwire tips in MEAs are often sharpened to reduce the insertion force and prevent the thin microwires from buckling. Currently, carbon fiber MEAs are sharpened by either torch burning, which limits the positions of wire tips to a water bath surface plane, or electrical discharge machining, which is difficult to implement to the nonelectrically conductive carbon fiber with parylene-C insulation. A laser-based carbon fiber sharpening method proposed in this study enables the fabrication of carbon fiber MEAs with sharp tips and custom lengths. Experiments were conducted to study effects of laser input voltage and transverse speed on carbon fiber tip geometry. Results of the tip sharpness and stripped length of the insulation as well as the electrochemical impedance spectroscopy measurement at 1 kHz were evaluated and analyzed. The laser input voltage and traverse speed have demonstrated to be critical for the sharp tip, short stripped length, and low electrical impedance of the carbon fiber electrode for brain recording MEAs. A carbon fiber MEA with custom electrode lengths was fabricated to validate the laser-based approach.

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