On-line determination by small angle X-ray scattering of the shape of hen egg white lysozyme immediately following elution from a hydrophobic interaction chromatography column

The Analyst ◽  
2016 ◽  
Vol 141 (20) ◽  
pp. 5810-5814 ◽  
Author(s):  
Chadin Kulsing ◽  
Andras Z. Komaromy ◽  
Reinhard I. Boysen ◽  
Milton T. W. Hearn
Keyword(s):  
Hydrophobic Interaction Chromatography ◽  
Integrated Approach ◽  
Egg White ◽  
Hen Egg White Lysozyme ◽  
X Ray ◽  
Egg White Lysozyme ◽  
X Ray Scattering ◽  
On Line ◽  
Hen Egg White ◽  
Hen Egg

This study documents the use of an integrated approach, involving on-line HIC interfaced with SAXS measurements, to monitor the conformational status of proteins immediately upon elution from a chromatographic column.

scholarly journals Ethanol-induced amyloid formation of hen egg-white lysozyme : a small-angle X-ray scattering study

2000 ◽  
Vol 40 (supplement) ◽  
pp. S115
Author(s):  
Y. Yonezawa ◽  
S. Tanaka ◽  
T. Kubota ◽  
K. Wakabayashi ◽  
K. Yutani ◽  
...  
Keyword(s):  
Small Angle ◽  
Egg White ◽  
Amyloid Formation ◽  
Hen Egg White Lysozyme ◽  
X Ray ◽  
Egg White Lysozyme ◽  
X Ray Scattering ◽  
Scattering Study ◽  
Hen Egg White ◽  
Hen Egg

scholarly journals Kinetics of the amyloid protofilament formation of hen egg-white lysozyme studied by small-angle X-ray scattering and CD measurements

2003 ◽  
Vol 43 (supplement) ◽  
pp. S66
Author(s):  
S. Fujiwara ◽  
Y. Yonezawa ◽  
S. Deshimaru ◽  
Y. Fujisawa ◽  
F. Matsumoto
Keyword(s):  
Small Angle ◽  
Egg White ◽  
Hen Egg White Lysozyme ◽  
X Ray ◽  
Egg White Lysozyme ◽  
X Ray Scattering ◽  
Hen Egg White ◽  
Kinetics Of ◽  
Hen Egg ◽  
Ray Scattering

scholarly journals Trifluoroethanol-induced conformational transition of hen egg-white lysozyme studied by small-angle X-ray scattering

FEBS Letters ◽  
1997 ◽  
Vol 416 (1) ◽  
pp. 72-76 ◽  
Author(s):  
Masaru Hoshino ◽  
Yoshihisa Hagihara ◽  
Daizo Hamada ◽  
Mikio Kataoka ◽  
Yuji Goto
Keyword(s):  
Small Angle ◽  
Conformational Transition ◽  
Egg White ◽  
Hen Egg White Lysozyme ◽  
X Ray ◽  
Egg White Lysozyme ◽  
X Ray Scattering ◽  
Hen Egg White ◽  
Hen Egg ◽  
Ray Scattering

Analysis of polycrystallinity in hen egg-white lysozyme using a pnCCD

2012 ◽  
Vol 45 (3) ◽  
pp. 517-522 ◽  
Author(s):  
Sebastian Send ◽  
Ali Abboud ◽  
Wolfram Leitenberger ◽  
Manfred S. Weiss ◽  
Robert Hartmann ◽  
...  
Keyword(s):  
Sample Surface ◽  
Egg White ◽  
Hen Egg White Lysozyme ◽  
X Ray ◽  
Lattice Constants ◽  
Egg White Lysozyme ◽  
Hen Egg White ◽  
Diffraction Patterns ◽  
Crystalline Domains ◽  
Hen Egg

A crystal of hen egg-white lysozyme was analyzed by means of energy-dispersive X-ray Laue diffraction with white synchrotron radiation at 2.7 Å resolution using a pnCCD detector. From Laue spots measured in a single exposure of the arbitrarily oriented crystal, the lattice constants of the tetragonal unit cell could be extracted with an accuracy of about 2.5%. Scanning across the sample surface, Laue images with split reflections were recorded at various positions. The corresponding diffraction patterns were generated by two crystalline domains with a tilt of about 1° relative to each other. The obtained results demonstrate the potential of the pnCCD for fast X-ray screening of crystals of macromolecules or proteins prior to conventional X-ray structure analysis. The described experiment can be automatized to quantitatively characterize imperfect single crystals or polycrystals.

Crystal distortion of monoclinic hen egg-white lysozyme crystals using X-ray digital topography

2014 ◽  
Vol 401 ◽  
pp. 238-241
Author(s):  
Kei Wako ◽  
Daiki Fujii ◽  
Shiro Tsukashima ◽  
Takeharu Kishi ◽  
Masaru Tachibana ◽  
...  
Keyword(s):  
Egg White ◽  
Hen Egg White Lysozyme ◽  
X Ray ◽  
Egg White Lysozyme ◽  
Hen Egg White ◽  
Lysozyme Crystals ◽  
Hen Egg

The metalation of hen egg white lysozyme impacts protein stability as shown by ion mobility mass spectrometry, differential scanning calorimetry, and X-ray crystallography

2017 ◽  
Vol 53 (30) ◽  
pp. 4246-4249 ◽  
Author(s):  
Matthew P. Sullivan ◽  
Michael Groessl ◽  
Samuel M. Meier ◽  
Richard L. Kingston ◽  
David C. Goldstone ◽  
...  
Keyword(s):  
Ion Mobility ◽  
Egg White ◽  
Ion Mobility Mass Spectrometry ◽  
Hen Egg White Lysozyme ◽  
Scanning Calorimetry ◽  
X Ray ◽  
X Ray Crystallography ◽  
Egg White Lysozyme ◽  
Hen Egg White ◽  
Hen Egg

Metalation of lysozyme with anticancer organometallics results in protein destabilisation, probably relevant in metallodrug mode of action.

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