Quantum dots modified with quaternized poly(dimethylaminoethyl methacrylate) for selective recognition and killing of bacteria over mammalian cells

The Analyst ◽  
2016 ◽  
Vol 141 (11) ◽  
pp. 3328-3336 ◽  
Author(s):  
Qin Tu ◽  
Chao Ma ◽  
Chang Tian ◽  
Maosen Yuan ◽  
Xiang Han ◽  
...  

Quantum dots modified with quaternized poly(dimethylaminoethyl methacrylate) were prepared by simple copper-free click chemistry for the selective recognition and killing of bacterial over mammalian cells.

2011 ◽  
Vol 23 (41) ◽  
pp. 4805-4810 ◽  
Author(s):  
Chunlei Zhu ◽  
Qiong Yang ◽  
Libing Liu ◽  
Fengting Lv ◽  
Shayu Li ◽  
...  

2017 ◽  
Vol 114 (8) ◽  
pp. E1365-E1374 ◽  
Author(s):  
Jennifer Greaves ◽  
Kevin R. Munro ◽  
Stuart C. Davidson ◽  
Matthieu Riviere ◽  
Justyna Wojno ◽  
...  

S-acylation is a major posttranslational modification, catalyzed by the zinc finger DHHC domain containing (zDHHC) enzyme family. S-acylated proteins can be modified by different fatty acids; however, very little is known about how zDHHC enzymes contribute to acyl chain heterogeneity. Here, we used fatty acid-azide/alkyne labeling of mammalian cells, showing their transformation into acyl-CoAs and subsequent click chemistry-based detection, to demonstrate that zDHHC enzymes have marked differences in their fatty acid selectivity. This difference in selectivity was apparent even for highly related enzymes, such as zDHHC3 and zDHHC7, which displayed a marked difference in their ability to use C18:0 acyl-CoA as a substrate. Furthermore, we identified isoleucine-182 in transmembrane domain 3 of zDHHC3 as a key determinant in limiting the use of longer chain acyl-CoAs by this enzyme. This study uncovered differences in the fatty acid selectivity profiles of cellular zDHHC enzymes and mapped molecular determinants governing this selectivity.


2018 ◽  
Vol 40 (2) ◽  
pp. 250-255 ◽  
Author(s):  
Savan K. Raj ◽  
Abhishek Rajput ◽  
Hariom Gupta ◽  
Rajesh Patidar ◽  
Vaibhav Kulshrestha

2004 ◽  
Vol 327 (2) ◽  
pp. 200-208 ◽  
Author(s):  
Larry C Mattheakis ◽  
Jennifer M Dias ◽  
Yun-Jung Choi ◽  
Jing Gong ◽  
Marcel P Bruchez ◽  
...  

2016 ◽  
Vol 52 (17) ◽  
pp. 3482-3485 ◽  
Author(s):  
Zhenhua Li ◽  
Zhen Liu ◽  
Zhaowei Chen ◽  
Enguo Ju ◽  
Wei Li ◽  
...  

We report a new strategy for selective recognition, separation and killing bacteria using metabolic engineering and bioorthogonal chemistry.


2015 ◽  
Vol 210 ◽  
pp. 349-354 ◽  
Author(s):  
Morteza Hosseini ◽  
Mohammad Reza Ganjali ◽  
Zahra Vaezi ◽  
Batool Arabsorkhi ◽  
Mehdi Dadmehr ◽  
...  

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