The presence of a 5′-abasic lesion enhances discrimination of single nucleotide polymorphisms while inducing an isothermal ligase chain reaction

The Analyst ◽  
2016 ◽  
Vol 141 (14) ◽  
pp. 4272-4277 ◽  
Author(s):  
Abu Kausar ◽  
Eiman A. Osman ◽  
Tendai Gadzikwa ◽  
Julianne M. Gibbs-Davis
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Dna Amplification ◽  
Nucleotide Polymorphisms ◽  
Chain Reaction ◽  
Single Nucleotide ◽  
Ligase Chain Reaction

Lesion-induced DNA amplification (LIDA) has been employed in the detection of single nucleotide polymorphisms (SNPs).

scholarly journals Correction: The presence of a 5′-abasic lesion enhances discrimination of single nucleotide polymorphisms while inducing an isothermal ligase chain reaction

The Analyst ◽  
2017 ◽  
Vol 142 (15) ◽  
pp. 2848-2848
Author(s):  
Abu Kausar ◽  
Eiman A. Osman ◽  
Tendai Gadzikwa ◽  
Julianne M. Gibbs-Davis
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Nucleotide Polymorphisms ◽  
Chain Reaction ◽  
Single Nucleotide ◽  
Ligase Chain Reaction

Correction for ‘The presence of a 5′-abasic lesion enhances discrimination of single nucleotide polymorphisms while inducing an isothermal ligase chain reaction’ by Abu Kausar et al., Analyst, 2016, 141, 4272–4277.

Ligase chain reaction-based electrochemical biosensor for the ultrasensitive and specific detection of single nucleotide polymorphisms

2019 ◽  
Vol 43 (36) ◽  
pp. 14327-14335 ◽  
Author(s):  
Wancun Zhang ◽  
Fang Hu ◽  
Xianwei Zhang ◽  
Wei Meng ◽  
Yaodong Zhang ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Signal Amplification ◽  
Electrochemical Biosensor ◽  
Nucleotide Polymorphisms ◽  
Specific Detection ◽  
Chain Reaction ◽  
Single Nucleotide ◽  
Ligase Chain Reaction ◽  
Amplification Strategy

In this study, a sensitive electrochemical biosensor for universally, robustly, specifically, and sensitively detecting SNPs was developed by using LCR as a signal amplification strategy.

Polymorphisms of LHβ and GnRHR genes and their association with the number of embryos recovered in goats

2014 ◽  
Vol 54 (8) ◽  
pp. 987 ◽  
Author(s):  
M. Z. Fu ◽  
G. Li ◽  
Z. Q. Zhou
Keyword(s):  
Polymerase Chain Reaction ◽  
Single Nucleotide Polymorphisms ◽  
Gene Polymorphism ◽  
Corpus Luteum ◽  
Nucleotide Polymorphisms ◽  
Chain Reaction ◽  
Single Strand Conformational Polymorphism ◽  
Single Nucleotide ◽  
Exon 2 ◽  
Polymerase Chain

The objective of the present study was to explore a predictor of superovulation response on the basis of associations between the number of embryos recovered and gene polymorphism. Variation in the goat LHβ and GnRHR genes was investigated using polymerase chain reaction–single-strand conformational polymorphism and DNA sequencing. Two single nucleotide polymorphisms (SNPs) were identified in the 5′-UTR of LHβ gene (A59C, P1 locus) and in the Exon 2 of GnRHR gene (T177A, P6 locus). At the P1 locus in both breeds, the frequencies of one allele were 0.46 and 0.51, respectively. At the P6 locus, the minor allele frequency was 0.23. Associations of both SNPs with the number of embryos recovered and the corpus luteum number were evaluated in Boer and Shaanbei goat breeds. Association analysis showed that both SNPs had significant (P < 0.05) effects on the number of embryos recovered and corpus luteum number. These results indicate that LHβ and GnRHR genes are potential markers for the number of embryos recovered.

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