A dual-mode signaling response of a AuNP-fluorescein based probe for specific detection of thiourea

The Analyst ◽  
2016 ◽  
Vol 141 (8) ◽  
pp. 2581-2587 ◽  
Author(s):  
Chuanxia Chen ◽  
Dan Zhao ◽  
Jian Sun ◽  
Xiurong Yang
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Selective Recognition ◽  
Quantitative Detection ◽  
Specific Detection ◽  
Dual Mode ◽  
Fluorescence Resonance ◽  
First Time

By employing fluorescein and AuNPs as energy donors and acceptors, respectively, a novel fluorescence resonance energy transfer (FRET)-based dual-mode sensor for selective recognition and quantitative detection of thiourea was designed and constructed in this study for the first time.

scholarly journals Ca2+dynamics in zebrafish morphogenesis

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e2894 ◽  
Author(s):  
Yusuke Tsuruwaka ◽  
Eriko Shimada ◽  
Kenta Tsutsui ◽  
Tomohisa Ogawa
Keyword(s):  
Energy Transfer ◽  
Intracellular Calcium ◽  
Fluorescence Resonance Energy Transfer ◽  
Calcium Ion ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Intracellular Calcium Ion ◽  
Fluorescence Resonance ◽  
First Time

Intracellular calcium ion (Ca2+) signaling is heavily involved in development, as illustrated by the use of a number of Ca2+indicators. However, continuous Ca2+patterns during morphogenesis have not yet been studied using fluorescence resonance energy transfer to track the Ca2+sensor. In the present study, we monitored Ca2+levels during zebrafish morphogenesis and differentiation with yellow cameleon, YC2.12. Our results show not only clear changes in Ca2+levels but also continuous Ca2+patterns at 24 hpf and later periods for the first time. Serial Ca2+dynamics during early pharyngula period (Prim-5-20; 24–33 hpf) was successfully observed with cameleon, which have not reported anywhere yet. In fact, high Ca2+level occurred concurrently with hindbrain development in segmentation and pharyngula periods. Ca2+patterns in the late gastrula through segmentation periods which were obtained with cameleon, were similar to those obtained previously with other Ca2+sensor. Our results suggested that the use of various Ca2+sensors may lead to novel findings in studies of Ca2+dynamics. We hope that these results will prove valuable for further research in Ca2+signaling.

scholarly journals Detection of Aflatoxins B1 in Maize Grains Using Fluorescence Resonance Energy Transfer

2020 ◽  
Vol 10 (5) ◽  
pp. 1578 ◽  
Author(s):  
Thanh Binh Nguyen ◽  
Thi Bich Vu ◽  
Hong Minh Pham ◽  
Cao Son Tran ◽  
Hong Hao Le Thi ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Fluorescence Lifetime ◽  
Aflatoxin B1 ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Fungal Species ◽  
Quantitative Detection ◽  
Acceptor Interaction ◽  
Fluorescence Resonance

Aflatoxins are secondary metabolites of Aspergillus flavus and Aspergillus parasiticus. These fungal species are the most dangerous and common toxin group causing food contamination. Aflatoxin has high toxicity and can cause cancer to humans and animals. The quantitative detection of aflatoxin in food, therefore, plays a very important role. However, in practice, due to low concentrations, aflatoxin detection analysis methods need to be highly sensitive and simple to apply. In this report, the fluorescence resonance energy transfer method (FRET) adopts the donor–acceptor interaction of aflatoxin B1. The CdSe/ZnS quantum dot detection of aflatoxin B1 will be presented wherein the aflatoxin B1 concentration can be determined from the changes in fluorescence lifetime or fluorescence intensity. A fluorescence lifetime calibration curve versus aflatoxin B1 concentrations was established. Test results of aflatoxin B1 determination in maize in Vietnam by FRET method are consistent with the results of aflatoxin B1 determination by HPLC based on ppm concentration.

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