scholarly journals Differential expression of serum proteins in rats subchronically exposed to arsenic identified by iTRAQ-based proteomic technology—14-3-3 ζ protein to serve as a potential biomarker

2016 ◽  
Vol 5 (2) ◽  
pp. 651-659 ◽  
Author(s):  
Jin Hui Zhang ◽  
Ying Li ◽  
Xuan Bo Song ◽  
Xiao Hong Ji ◽  
Hong Na Sun ◽  
...  
Keyword(s):  
Differential Expression ◽  
Serum Proteins ◽  
Proteomic Technology ◽  
Potential Biomarker

Arsenic is a multi-system toxicant.

A differential expression of miRNA in plasma and breast tissue: A potential biomarker

2016 ◽  
Vol 42 (5) ◽  
pp. S20
Author(s):  
M.Arif Nasir ◽  
Zaynab Abdul-Ghany ◽  
Zartasht Carmichael ◽  
James E.P. Brown ◽  
Amtul R. Carmichael
Keyword(s):  
Differential Expression ◽  
Breast Tissue ◽  
Potential Biomarker

scholarly journals Serum biomarkers discovery of steroid-induced femoral head osteonecrosis in Chinese female by comparative proteome

2020 ◽  
Author(s):  
Ping Zeng ◽  
Chongxing Zhang ◽  
Yanqiong Zhou ◽  
Jinyi Li ◽  
Tao Li ◽  
...  
Keyword(s):  
Femoral Head ◽  
Healthy Volunteers ◽  
Differential Expression ◽  
Western Blotting ◽  
Serum Proteins ◽  
Cell Lineage ◽  
Hematopoietic Cell ◽  
Diagnostic Biomarkers ◽  
Fat Digestion ◽  
Hematopoietic Cell Lineage

Abstract Background Steroid-induced osteonecrosis of the femoral head (SONFH) is a disabling, aseptic and ischemic disease due to excessive glucocorticoids (GCs) usage. Patients with SONFH are commonly asymptomatic, which makes its early diagnosis is challenge, the pathological mechanisms of SONFH are not well-known, the purpose of the present study was to screen diagnostic biomarkers for SONFH. Methods The differential expression of serum proteins from SONFH, traumatic osteonecrosis of the femoral head (TONFH) patients and healthy volunteers (CK) in Chinese females was compared using iTRAQ, and potential diagnostic biomarkers were verified by western blotting. Results Gene Ontology, Kyoto Encyclopedia of Genes and Genomes (KEGG), Domain and Clusters of Orthologous Groups (COG) analyses revealed key groups of proteins, pathways and domains differentially regulated among SONFH, TONFH and healthy volunteers in Chinese female, the results showed that peptidase S1, fibrinogen, transferrin, lipid transport domains and hematopoietic cell lineage, fat digestion, absorption, peroxisome proliferator-activated receptor (PPAR) pathways were associated with the development of SONFH. Finally, C-reactive protein (CRP), serum amyloid A protein (SAA1), alpha-1-acid glycoprotein 1 (ORM1) and dopamine beta-hydroxylase were selected for verification of differential expression using western blotting. Conclusions Our data suggest that dysfunction of hematopoietic cell lineage, adhesion, fat digestion and absorption, PPAR pathways may be involved in the pathogenesis of SONFH, serum proteins SAA1, ORM1 could be used as new potential diagnostic biomarkers for SONFH.

Analysis of genetic mutations associated with immune-activated microenvironment in lung adenocarcinoma.

2021 ◽  
Vol 39 (15_suppl) ◽  
pp. e20520-e20520
Author(s):  
Zhen Liang ◽  
Shuhua Zhao ◽  
Danni Liu ◽  
Tianhao Mu ◽  
Jinfeng Chen
Keyword(s):  
Lung Adenocarcinoma ◽  
Differential Expression ◽  
Mutation Frequency ◽  
Immune Cell ◽  
Differential Expression Analysis ◽  
Gene List ◽  
Genetic Mutation ◽  
Rank Test ◽  
P Value ◽  
Potential Biomarker

e20520 Background: Tumor microenvironment plays an important role in suppressing or enhancing immune response. However, the relationship between genetic mutation and prognosis of lung adenocarcinoma (LUAD) patients, and the molecular mechanism of immune microenvironment are not completely clear. Methods: Expression matrix and somatic mutations of TCGA LUAD (n = 509) were downloaded from UCSC Xena. Immune-related gene list was downloaded from ImmPort. We applied R packages ESTIMATE and CIBERSORT to calculate the proportion of tumor-infiltrating immune cell by mRNA data. Samples were divided into ImmuneScore-High or ImmuneScore-Low depending on upper or lower quartiles of ESTIMATE immune infiltration score. Differential expression analysis between ImmuneScore-High and ImmuneScore-Low was performed with R package DESeq2. Results: Samples were divided into ImmuneScore-High (n = 127) and ImmuneScore-Low (n = 128). The immune cell components of these two groups presented different patterns. 10 most frequently mutated immune-related genes were significantly different between two groups (Chi-Squared test, p value < 0.05). 4,139 differentially expressed genes were obtained (|log2FC| > 1, FDR < 0.05). IL-7R, PIK3R5, TRBC2 and VCAM1 were identified with differential mutation frequency and presented differential expression between two groups. Kaplan-Meier plot in UALCAN cancer database showed IL-7R had a significant influence on the prognosis of patients (log rank test, p value = 0.045). Conclusions: ImmuneScore-High and ImmunScore-Low groups presented different genetic mutation frequency and gene expression. Moreover, mutation and different expression of IL-7R may be used as a potential biomarker in the treatment of patients with lung adenocarcinoma.

Differential Expression of Cohesin SA in Early Colorectal Carcinogenesis: Potential Biomarker for Racial Disparities

2014 ◽  
Vol 109 ◽  
pp. S612
Author(s):  
Ehsan Chitsaz ◽  
Mart DeLaCruz ◽  
Audrey Calderwood ◽  
Ramesh Wali ◽  
Navneet Momi ◽  
...  
Keyword(s):  
Racial Disparities ◽  
Differential Expression ◽  
Colorectal Carcinogenesis ◽  
Potential Biomarker

scholarly journals Plasma extracellular vesicle miRNAs as potential biomarkers of superstimulatory response in cattle

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Ahmed Gad ◽  
José María Sánchez ◽  
John A. Browne ◽  
Lucie Nemcova ◽  
Jozef Laurincik ◽  
...  
Keyword(s):  
Differential Expression ◽  
Extracellular Vesicle ◽  
Beef Heifers ◽  
Blood Samples ◽  
Time Points ◽  
Differential Expression Pattern ◽  
Potential Biomarker ◽  
Before And After ◽  
Mirna Abundance ◽  
Small Rnaseq

Abstract The ability to predict superstimulatory response would be a beneficial tool in assisted reproduction. Using small RNAseq technology, we profiled extracellular vesicle microRNA (EV-miRNA) abundance in the blood plasma of heifers exhibiting variable responses to superstimulation. Estrous synchronized crossbred beef heifers (n = 25) were superstimulated and blood samples were collected from each heifer on Day 7 of consecutive unstimulated (U) and superstimulated (S) cycles. A subset of high (H) and low (L) responders was selected depending on their response to superstimulation and EV-miRNA profiles were analysed at both time-points in each heifer. Approximately 200 known miRNAs were detected in each sample with 144 commonly detected in all samples. A total of 12 and 14 miRNAs were dysregulated in UH vs. UL and in SH vs. SL heifers, respectively. Interestingly, miR-206 and miR-6517 exhibited the same differential expression pattern in H compared to L heifers both before and after superstimulation. Pathway analysis indicated that circadian rhythm and signaling pathways were among the top pathways enriched with genes targeted by dysregulated miRNAs in H vs. L responding heifers. In conclusion, heifers with divergent ovarian responses exhibited differential expression of plasma EV-miRNAs which may be used as a potential biomarker to predict superstimulation response.

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