scholarly journals Pancreatic progenitor-derived hepatocytes are viable and functional in a 3D high density bioreactor culture system

2016 ◽  
Vol 5 (1) ◽  
pp. 278-290 ◽  
Author(s):  
M. Richter ◽  
E. A. Fairhall ◽  
S. A. Hoffmann ◽  
S. Tröbs ◽  
F. Knöspel ◽  
...  
Keyword(s):  
Drug Metabolism ◽  
Cell Line ◽  
Culture System ◽  
Progenitor Cell ◽  
High Density ◽  
Bioreactor Culture ◽  
Pancreatic Progenitor ◽  
Pancreatic Progenitor Cell ◽  
System P ◽  
Progenitor Cell Line

The rat pancreatic progenitor cell line B-13 is of interest for research on drug metabolism and toxicity since the cells trans-differentiate into functional hepatocyte-like cells (B-13/H) when treated with glucocorticoids.

scholarly journals Generation of hepatocytes expressing functional cytochromes P450 from a pancreatic progenitor cell line in vitro

2003 ◽  
Vol 370 (3) ◽  
pp. 763-769 ◽  
Author(s):  
Carylyn J. MAREK ◽  
Gary A. CAMERON ◽  
Lucy J. ELRICK ◽  
Gabrielle M. HAWKSWORTH ◽  
Matthew C. WRIGHT
Keyword(s):  
Cell Line ◽  
Progenitor Cell ◽  
Cytochromes P450 ◽  
Glucocorticoid Treatment ◽  
Pancreatic Cell ◽  
Pancreatic Progenitor ◽  
Albumin Gene ◽  
Pancreatic Progenitor Cell ◽  
Progenitor Cell Line

The proliferating AR42J-B13 pancreatic cell line is known to respond to glucocorticoid treatment by producing foci of cells that express the liver-specific albumin gene. We demonstrate that this cell line also expresses liver-specific or liver-enriched functional cytochrome P450 proteins when stimulated to trans-differentiate into hepatocytes by glucocorticoid. These data suggest that this cell line has an unusual ability to trans-differentiate into functional hepatocytes and that it could be possible to generate a limitless supply of functional hepatocyte-like cells in vitro.

Downregulation of c-kit (Stem Cell Factor Receptor) in Transformed Hematopoietic Precursor Cells by Stroma Cells

Blood ◽  
1999 ◽  
Vol 93 (2) ◽  
pp. 554-563 ◽  
Author(s):  
Christoph Heberlein ◽  
Jutta Friel ◽  
Christine Laker ◽  
Dorothee von Laer ◽  
Ulla Bergholz ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Line ◽  
Stem Cell Factor ◽  
Progenitor Cell ◽  
Receptor Expression ◽  
General Mechanism ◽  
Ligand Interaction ◽  
Kit Ligand ◽  
Kit Receptor ◽  
Progenitor Cell Line

Abstract We show a dramatic downregulation of the stem cell factor (SCF) receptor in different hematopoietic cell lines by murine stroma. Growth of the human erythroid/macrophage progenitor cell line TF-1 is dependent on granulocyte-macrophage colony-stimulating factor (GM-CSF) or interleukin-3 (IL-3). However, TF-1 cells clone and proliferate equally well on stroma. Independent stroma-dependent TF-1 clones (TF-1S) were generated on MS-5 stroma. Growth of TF-1S and TF-1 cells on stroma still requires interaction between c-kit (SCF receptor) and its ligand SCF, because antibodies against c-kit inhibit growth to less than 2%. Surprisingly, c-kit receptor expression (RNA and protein) was downregulated by 2 to 3 orders of magnitude in TF-1S and TF-1 cells grown on stroma. This stroma-dependent regulation of the kit receptor in TF-1 was also observed on exposure to kit ligand-negative stroma, thus indicating the need for heterologous receptor ligand interaction. Removal of stroma induced upregulation by 2 to 4 orders of magnitude. Downregulation and upregulation of c-kit expression could also be shown for the megakaryocytic progenitor cell line M-07e and was comparable to that of TF-1, indicating that stroma-dependent regulation of c-kit is a general mechanism. Downregulation may be an economic way to compensate for the increased sensitivity of the c-kit/ligand interaction on stroma. The stroma-dependent c-kit regulation most likely occurs at the transcriptional level, because mechanisms, such as splicing, attenuation, differential promoter usage, or mRNA stability, could be excluded.

An attempt to generate neurons from an astrocyte progenitor cell line FBD-104

2005 ◽  
Vol 53 (2) ◽  
pp. 104-115 ◽  
Author(s):  
Makoto Horiuchi ◽  
Yasuhiro Tomooka
Keyword(s):  
Cell Line ◽  
Progenitor Cell ◽  
Progenitor Cell Line

Isolation and characterization of a variant myoblast cell line that is temperature sensitive for differentiation

1988 ◽  
Vol 8 (6) ◽  
pp. 2335-2341
Author(s):  
R J Akhurst ◽  
N B Flavin ◽  
J Worden
Keyword(s):  
Cell Line ◽  
Progenitor Cell ◽  
Temperature Shift ◽  
Temperature Sensitive ◽  
Isolation And Characterization ◽  
New Variant ◽  
Myoblast Cell Line ◽  
Myoblast Cell ◽  
Progenitor Cell Line

A new variant rat myogenic cell line, ts485, was isolated by subcloning the cell line ts3b2 (H. T. Nguyen, R. M. Medford, and B. Nadal-Ginard, Cell 34:281-293, 1983). Unlike the progenitor cell line, ts485 was thermosensitive for differentiation. Experiments with conditioned medium suggested that diffusible extracellular factors were not involved in dictating the differential phenotypes of ts485 cells cultured at the permissive and nonpermissive temperatures. Temperature shift experiments performed on cultures of ts485 cells indicated that the temperature-sensitive lesion was in a factor active during the growth phase and required to trigger a cascade of events leading to terminal differentiation.

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