Solid supported lipid bilayers from artificial and natural lipid mixtures – long-term stable, homogeneous and reproducible

2015 ◽  
Vol 3 (29) ◽  
pp. 6046-6056 ◽  
Author(s):  
Isabelle Möller ◽  
Stefan Seeger
Keyword(s):  
Lipid Bilayers ◽  
Supported Lipid Bilayers ◽  
Deposition Method ◽  
Flow Conditions ◽  
Lipid Mixtures ◽  
Natural Lipid

We show the assembly of reproducible, long-term stable, homogeneous solid supported lipid bilayers under flow conditions by the vesicle deposition method from various artificial and natural lipid mixtures.

Influence of Brain Gangliosides on the Formation and Properties of Supported Lipid Bilayers for Binding Kinetics Studies

2018 ◽  
Author(s):  
Luke Jordan ◽  
Nathan Wittenberg
Keyword(s):  
Lipid Bilayers ◽  
Binding Kinetics ◽  
Supported Lipid Bilayers ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Supported Lipid Bilayer ◽  
Head Groups ◽  
Lipid Diffusion ◽  
Kinetics Of ◽  
Brain Gangliosides

This is a comprehensive study of the effects of the four major brain gangliosides (GM1, GD1b, GD1a, and GT1b) on the adsorption and rupture of phospholipid vesicles on SiO2 surfaces for the formation of supported lipid bilayer (SLB) membranes. Using quartz crystal microbalance with dissipation monitoring (QCM-D) we show that gangliosides GD1a and GT1b significantly slow the SLB formation process, whereas GM1 and GD1b have smaller effects. This is likely due to the net ganglioside charge as well as the positions of acidic sugar groups on ganglioside glycan head groups. Data is included that shows calcium can accelerate the formation of ganglioside-rich SLBs. Using fluorescence recovery after photobleaching (FRAP) we also show that the presence of gangliosides significantly reduces lipid diffusion coefficients in SLBs in a concentration-dependent manner. Finally, using QCM-D and GD1a-rich SLB membranes we measure the binding kinetics of an anti-GD1a antibody that has similarities to a monoclonal antibody that is a hallmark of a variant of Guillain-Barre syndrome.

scholarly journals Silica nanoparticle supported lipid bilayers for gene delivery

2009 ◽  
pp. 5100 ◽  
Author(s):  
Juewen Liu ◽  
Alison Stace-Naughton ◽  
C. Jeffrey Brinker
Keyword(s):  
Gene Delivery ◽  
Lipid Bilayers ◽  
Silica Nanoparticle ◽  
Supported Lipid Bilayers

scholarly journals Formation of Supported Lipid Bilayers Derived from Vesicles of Various Compositional Complexity on Conducting Polymer/Silica Substrates

Langmuir ◽  
2021 ◽  
Author(s):  
Hanna Ulmefors ◽  
Josefin Nissa ◽  
Hudson Pace ◽  
Olov Wahlsten ◽  
Anders Gunnarsson ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Conducting Polymer ◽  
Supported Lipid Bilayers ◽  
Silica Substrates

scholarly journals Delivery of Ricin Toxin A-Chain by Peptide-Targeted Mesoporous Silica Nanoparticle-Supported Lipid Bilayers

2012 ◽  
Vol 1 (3) ◽  
pp. 348-353 ◽  
Author(s):  
Katharine Epler ◽  
David Padilla ◽  
Genevieve Phillips ◽  
Peter Crowder ◽  
Robert Castillo ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Lipid Bilayers ◽  
Silica Nanoparticle ◽  
Supported Lipid Bilayers ◽  
Toxin A ◽  
Mesoporous Silica Nanoparticle ◽  
Ricin Toxin ◽  
A Chain

scholarly journals Enhanced Long-Term Stability for Single Ion Channel Recordings Using Suspended Poly(lipid) Bilayers

2009 ◽  
Vol 131 (19) ◽  
pp. 6662-6663 ◽  
Author(s):  
Benjamin A. Heitz ◽  
Juhua Xu ◽  
Henry K. Hall ◽  
Craig A. Aspinwall ◽  
S. Scott Saavedra
Keyword(s):  
Ion Channel ◽  
Lipid Bilayers ◽  
Term Stability ◽  
Long Term Stability

scholarly journals Systematic Measurements of Interleaflet Friction in Supported Lipid Bilayers

2021 ◽  
Vol 120 (3) ◽  
pp. 146a
Author(s):  
Autumn A. Anthony ◽  
Aurelia R. Honerkamp-Smith
Keyword(s):  
Lipid Bilayers ◽  
Supported Lipid Bilayers
Sign in / Sign up

Export Citation Format

Share Document