scholarly journals Carbon nanotube uptake changes the biomechanical properties of human lung epithelial cells in a time-dependent manner

2015 ◽  
Vol 3 (19) ◽  
pp. 3983-3992 ◽  
Author(s):  
Chenbo Dong ◽  
Reem Eldawud ◽  
Linda M. Sargent ◽  
Michael L. Kashon ◽  
David Lowry ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Exposure Duration ◽  
Human Lung ◽  
Biomechanical Properties ◽  
Engineered Nanomaterials ◽  
Lung Epithelial Cells ◽  
Time Dependent ◽  
Dependent Manner ◽  
Lung Epithelial ◽  
Human Lung Epithelial Cells

The toxicity of engineered nanomaterials in biological systems depends on both the nanomaterial properties and the exposure duration.

scholarly journals Thrombin Induces NF-κB Activation and IL-8/CXCL8 Expression in Lung Epithelial Cells by a Rac1-dependent PI3K/Akt Pathway

2011 ◽  
Vol 286 (12) ◽  
pp. 10483-10494 ◽  
Author(s):  
Chien-Huang Lin ◽  
Hui-Wen Cheng ◽  
Hon-Ping Ma ◽  
Chih-Hsiung Wu ◽  
Chuang-Ye Hong ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Human Lung ◽  
Luciferase Activity ◽  
Lung Epithelial Cells ◽  
Akt Pathway ◽  
Dependent Manner ◽  
Akt Inhibitor ◽  
Iκb Kinase ◽  
Lung Epithelial ◽  
Human Lung Epithelial Cells

We previously showed that thrombin induces interleukin (IL)-8/CXCL8 expression via the protein kinase C (PKC)α/c-Src-dependent IκB kinase α/β (IKKα/β)/NF-κB signaling pathway in human lung epithelial cells. In this study, we further investigated the roles of Rac1, phosphoinositide 3-kinase (PI3K), and Akt in thrombin-induced NF-κB activation and IL-8/CXCL8 expression. Thrombin-induced IL-8/CXCL8 release and IL-8/CXCL8-luciferase activity were attenuated by a PI3K inhibitor (LY294002), an Akt inhibitor (1-l-6-hydroxymethyl-chiro-inositol-2-((R)-2-O-methyl-3-O-octadecylcarbonate)), and the dominant negative mutants of Rac1 (RacN17) and Akt (AktDN). Treatment of cells with thrombin caused activation of Rac and Akt. The thrombin-induced increase in Akt activation was inhibited by RacN17 and LY294002. Stimulation of cells with thrombin resulted in increases in IKKα/β activation and κB-luciferase activity; these effects were inhibited by RacN17, LY294002, an Akt inhibitor, and AktDN. Treatment of cells with thrombin induced Gβγ, p85α, and Rac1 complex formation in a time-dependent manner. These results imply that thrombin activates the Rac1/PI3K/Akt pathway through formation of the Gβγ, Rac1, and p85α complex to induce IKKα/β activation, NF-κB transactivation, and IL-8/CXCL8 expression in human lung epithelial cells.

Human lung epithelial cells show biphasic oxidative burst after single-walled carbon nanotube contact

Carbon ◽  
2007 ◽  
Vol 45 (11) ◽  
pp. 2241-2249 ◽  
Author(s):  
Karin Pulskamp ◽  
Jörg M. Wörle-Knirsch ◽  
Frank Hennrich ◽  
Katrin Kern ◽  
Harald F. Krug
Keyword(s):  
Carbon Nanotube ◽  
Epithelial Cells ◽  
Oxidative Burst ◽  
Human Lung ◽  
Lung Epithelial Cells ◽  
Single Walled Carbon Nanotube ◽  
Single Walled Carbon ◽  
Lung Epithelial ◽  
Human Lung Epithelial Cells

scholarly journals Towards elucidating the effects of purified MWCNTs on human lung epithelial cells

2014 ◽  
Vol 1 (6) ◽  
pp. 595-603 ◽  
Author(s):  
Chenbo Dong ◽  
Reem Eldawud ◽  
Linda M. Sargent ◽  
Michael L. Kashon ◽  
David Lowry ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Human Lung ◽  
Biomechanical Properties ◽  
Lung Epithelial Cells ◽  
Mitochondrial Activity ◽  
Cycle Arrest ◽  
Lung Epithelial ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes ◽  
Human Lung Epithelial Cells

Exposure to purified multi-walled carbon nanotubes (MWCNTs) induced cell cycle arrest, reduced mitochondrial activity and changed cellular biomechanical properties.

Nitrative DNA damage in human lung epithelial cells treated with multi-walled carbon nanotube

Nitric Oxide ◽  
2012 ◽  
Vol 27 ◽  
pp. S39
Author(s):  
Feiye Guo ◽  
Ning Ma ◽  
Shosuke Kawanishi ◽  
Mariko Murata ◽  
Yusuke Hiraku
Keyword(s):  
Dna Damage ◽  
Carbon Nanotube ◽  
Epithelial Cells ◽  
Human Lung ◽  
Lung Epithelial Cells ◽  
Multi Walled Carbon Nanotube ◽  
Lung Epithelial ◽  
Human Lung Epithelial Cells

Nitrative DNA damage induced by multi-walled carbon nanotube via endocytosis in human lung epithelial cells

2012 ◽  
Vol 260 (2) ◽  
pp. 183-192 ◽  
Author(s):  
Feiye Guo ◽  
Ning Ma ◽  
Yoshiteru Horibe ◽  
Shosuke Kawanishi ◽  
Mariko Murata ◽  
...  
Keyword(s):  
Dna Damage ◽  
Carbon Nanotube ◽  
Epithelial Cells ◽  
Human Lung ◽  
Lung Epithelial Cells ◽  
Multi Walled Carbon Nanotube ◽  
Lung Epithelial ◽  
Human Lung Epithelial Cells

Legionella pneumophila induced microRNA expression in human lung epithelial cells

Pneumologie ◽  
2010 ◽  
Vol 64 (S 03) ◽  
Author(s):  
B Schmeck ◽  
B Dolniak ◽  
I Pollock ◽  
C Schulz ◽  
W Bertrams ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Legionella Pneumophila ◽  
Human Lung ◽  
Microrna Expression ◽  
Lung Epithelial Cells ◽  
Lung Epithelial ◽  
Human Lung Epithelial Cells
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