scholarly journals Characterizing anomalous diffusion in crowded polymer solutions and gels over five decades in time with variable-lengthscale fluorescence correlation spectroscopy

Soft Matter ◽  
2016 ◽  
Vol 12 (18) ◽  
pp. 4190-4203 ◽  
Author(s):  
Daniel S. Banks ◽  
Charmaine Tressler ◽  
Robert D. Peters ◽  
Felix Höfling ◽  
Cécile Fradin
Keyword(s):  
Anomalous Diffusion ◽  
Fluorescence Correlation Spectroscopy ◽  
Polymer Solutions ◽  
Tracer Diffusion ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
Wide Range

FCS with a wide range of beam waists was used to measure tracer diffusion in crowded media over five decades in time, thus providing a strong test for different models of anomalous diffusion.

Fluorescence correlation spectroscopy: The technique and its applications in soft matter

2018 ◽  
Vol 4 (4) ◽  
Author(s):  
Anjali Gupta ◽  
Jagadish Sankaran ◽  
Thorsten Wohland
Keyword(s):  
Single Molecule ◽  
Fluorescence Correlation Spectroscopy ◽  
Soft Matter ◽  
Photophysical Properties ◽  
Spatiotemporal Analysis ◽  
Correlation Spectroscopy ◽  
Basic Principles ◽  
Chemical Reaction Kinetics ◽  
Fluorescence Correlation ◽  
Wide Range

Abstract Fluorescence correlation spectroscopy (FCS) is a well-established single-molecule method used for the quantitative spatiotemporal analysis of dynamic processes in a wide range of samples. It possesses single-molecule sensitivity but provides ensemble averaged molecular parameters such as mobility, concentration, chemical reaction kinetics, photophysical properties and interaction properties. These parameters have been utilized to characterize a variety of soft matter systems. This review provides an overview of the basic principles of various FCS modalities, their instrumentation, data analysis, and the applications of FCS to soft matter systems.

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