scholarly journals Minimization of dynamic effects in the evolution of dihydrofolate reductase

2016 ◽  
Vol 7 (5) ◽  
pp. 3248-3255 ◽  
Author(s):  
J. Javier Ruiz-Pernía ◽  
Enas Behiry ◽  
Louis Y. P. Luk ◽  
E. Joel Loveridge ◽  
Iñaki Tuñón ◽  
...  
Keyword(s):  
Dihydrofolate Reductase ◽  
Enzyme Catalysis ◽  
Isotope Labeling ◽  
Conformational Dynamics ◽  
Dynamic Effects ◽  
Powerful Technique ◽  
P Protein

Protein isotope labeling is a powerful technique to probe functionally important motions in enzyme catalysis and can be applied to investigate the conformational dynamics of proteins.

scholarly journals Protein motions and dynamic effects in enzyme catalysis

2015 ◽  
Vol 17 (46) ◽  
pp. 30817-30827 ◽  
Author(s):  
Louis Y. P. Luk ◽  
E. Joel Loveridge ◽  
Rudolf K. Allemann
Keyword(s):  
Dihydrofolate Reductase ◽  
Enzyme Catalysis ◽  
Catalytic Cycle ◽  
Dynamic Effects ◽  
Dynamic Coupling ◽  
Protein Motions ◽  
Catalytic Power

While the full catalytic power of dihydrofolate reductase depends on finely tuning protein motions in each step of the catalytic cycle, dynamic coupling to the actual chemical step is detrimental to catalysis.

scholarly journals Chemical Ligation and Isotope Labeling to Locate Dynamic Effects during Catalysis by Dihydrofolate Reductase

2015 ◽  
Vol 127 (31) ◽  
pp. 9144-9148 ◽  
Author(s):  
Louis Y. P. Luk ◽  
J. Javier Ruiz-Pernía ◽  
Aduragbemi S. Adesina ◽  
E. Joel Loveridge ◽  
Iñaki Tuñón ◽  
...  
Keyword(s):  
Dihydrofolate Reductase ◽  
Isotope Labeling ◽  
Dynamic Effects ◽  
Chemical Ligation

scholarly journals Chemical Ligation and Isotope Labeling to Locate Dynamic Effects during Catalysis by Dihydrofolate Reductase

2015 ◽  
Vol 54 (31) ◽  
pp. 9016-9020 ◽  
Author(s):  
Louis Y. P. Luk ◽  
J. Javier Ruiz-Pernía ◽  
Aduragbemi S. Adesina ◽  
E. Joel Loveridge ◽  
Iñaki Tuñón ◽  
...  
Keyword(s):  
Dihydrofolate Reductase ◽  
Isotope Labeling ◽  
Dynamic Effects ◽  
Chemical Ligation

Dihydrofolate Reductase as a Model for Enzyme Catalysis

2015 ◽  
Vol 4 (2) ◽  
pp. 77-86 ◽  
Author(s):  
Kevin Francis ◽  
Amnon Kohen
Keyword(s):  
Dihydrofolate Reductase ◽  
Enzyme Catalysis

How adding a single methylene to dihydrofolate reductase can change its conformational dynamics

2021 ◽  
Vol 154 (16) ◽  
pp. 165103
Author(s):  
Ryan W. Penhallurick ◽  
Alliyah Harold ◽  
Maya D. Durnal ◽  
Toshiko Ichiye
Keyword(s):  
Dihydrofolate Reductase ◽  
Conformational Dynamics

scholarly journals Rescue of conformational dynamics in enzyme catalysis by directed evolution

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Renee Otten ◽  
Lin Liu ◽  
Lillian R. Kenner ◽  
Michael W. Clarkson ◽  
David Mavor ◽  
...  
Keyword(s):  
Directed Evolution ◽  
Enzyme Catalysis ◽  
Conformational Dynamics
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