scholarly journals Wide-field tissue polarimetry allows efficient localized mass spectrometry imaging of biological tissues

2016 ◽  
Vol 7 (3) ◽  
pp. 2162-2169 ◽  
Author(s):  
Alessandra Tata ◽  
Adam Gribble ◽  
Manuela Ventura ◽  
Milan Ganguly ◽  
Emma Bluemke ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Biological Tissues ◽  
Wide Field ◽  
Conventional Methods

Targeted and localized mass spectrometry imaging allows faster characterization of cancer compared to conventional methods.

scholarly journals MALDI-TOF/TOF Tandem Mass Spectrometry Imaging Reveals Non-uniform Distribution of Disaccharide Isomers in Plant Tissues

2020 ◽  
Author(s):  
lingpeng zhan ◽  
xi huang ◽  
jinjuan xue ◽  
huihui liu ◽  
caiqiao xiong ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Biological Tissues ◽  
Plant Tissues ◽  
Tandem Mass ◽  
Imaging Analysis ◽  
Maldi Tof ◽  
Ms Imaging ◽  
Imaging Approach

Mass spectrometry imaging (MSI) has been increasingly utilized in investigating the locations of biomolecules within tissues. However, the isomeric compounds are rarely distinguished in the MS images, due to inability of MSI methods to differentiate isomers in the probing area. Coupling tandem mass spectrometry with MSI can facilitate differentiating isomeric compounds in ion images. Here we apply MALDI-TOF/TOF tandem mass spectrometry imaging approach to revealing the spatial distributions of isomeric disaccharides in plant tissues. First, the MS/MS imaging analysis of disaccharide-matrix droplet spots demonstrated the feasibility of distinguishing isomeric species in tissues, by measuring the relative intensity of specific fragments. Then, we conducted tandem MS imaging of disaccharides in onion bulb tissues, which indicated that sucrose and other unknown non-sucrose disaccharides exhibit heterogeneous locations throughout the tissues. This method enables us to image disaccharide isomers differentially in biological tissues, and to discover new saccharide species in plant. This work also emphasizes the necessity of considering isobaric compounds when interpreting MSI results.<br>

scholarly journals Mass spectrometry imaging of triglycerides in biological tissues by laser desorption ionization from silicon nanopost arrays

2019 ◽  
Vol 55 (4) ◽  
pp. e4443 ◽  
Author(s):  
Jarod A. Fincher ◽  
Andrew R. Korte ◽  
Jacqueline E. Dyer ◽  
Sridevi Yadavilli ◽  
Nicholas J. Morris ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Laser Desorption ◽  
Biological Tissues ◽  
Laser Desorption Ionization ◽  
Desorption Ionization

Characterization of Metabolite Compositions in Wild and Farmed Red Sea Bream (Pagrus major) Using Mass Spectrometry Imaging

2019 ◽  
Vol 67 (25) ◽  
pp. 7197-7203 ◽  
Author(s):  
Naoko Goto-Inoue ◽  
Tomohiko Sato ◽  
Mizuki Morisasa ◽  
Yuika Igarashi ◽  
Tsukasa Mori
Keyword(s):  
Mass Spectrometry ◽  
Red Sea ◽  
Mass Spectrometry Imaging ◽  
Sea Bream ◽  
Red Sea Bream ◽  
Pagrus Major

scholarly journals Quantitative Mass Spectrometry Imaging of Biological Systems

2021 ◽  
Vol 72 (1) ◽  
Author(s):  
Daisy Unsihuay ◽  
Daniela Mesa Sanchez ◽  
Julia Laskin
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Matrix Effects ◽  
High Sensitivity ◽  
Biological Tissues ◽  
Annual Review ◽  
Publication Date ◽  
Label Free ◽  
Complex Samples ◽  
Mass Dependent

Mass spectrometry imaging (MSI) is a powerful, label-free technique that provides detailed maps of hundreds of molecules in complex samples with high sensitivity and subcellular spatial resolution. Accurate quantification in MSI relies on a detailed understanding of matrix effects associated with the ionization process along with evaluation of the extraction efficiency and mass-dependent ion losses occurring in the analysis step. We present a critical summary of approaches developed for quantitative MSI of metabolites, lipids, and proteins in biological tissues and discuss their current and future applications. Expected final online publication date for the Annual Review of Physical Chemistry, Volume 72 is April 2021. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

Adhesive film applications help to prepare strawberry fruit sections for desorption electrospray ionization-mass spectrometry imaging

2021 ◽  
Author(s):  
Hirofumi Enomoto
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Electrospray Ionization Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Desorption Electrospray Ionization ◽  
Biological Tissues ◽  
Ionization Mass Spectrometry ◽  
High Water Content ◽  
Ionization Mass ◽  
Adhesive Film

ABSTRACT Desorption electrospray ionization-mass spectrometry imaging (DESI-MSI) is a powerful tool to analyze the distribution of metabolites in biological tissues. Cryosectioning of biological tissues is usually required prior to DESI-MSI, but it can be difficult for tissues that are fragile, hard, and have a high-water content. The Kawamoto method uses transparent adhesive films to prepare cryosections; however, its application for plant tissues, such as strawberry tissues, in DESI-MSI has not been verified. In this study, strawberry cryosections maintained original structures were prepared using adhesive film. Subsequently, numerous peaks were detected for the sections using the positive and negative ion modes of DESI-MSI. Several primary and specialized metabolites, such as amino acids, sugars, organic acids, and flavonoids, were identified and visualized. These results suggest the use of adhesive films when cryosectioning could improve DESI-MSI analysis of the metabolites in strawberry fruits and various tissues of other plant species.

scholarly journals In Situ Mass Spectrometry Imaging and Ex Vivo Characterization of Renal Crystalline Deposits Induced in Multiple Preclinical Drug Toxicology Studies

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e47353 ◽  
Author(s):  
Anna Nilsson ◽  
Benita Forngren ◽  
Sivert Bjurström ◽  
Richard J. A. Goodwin ◽  
Elisa Basmaci ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Ex Vivo ◽  
Crystalline Deposits
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