scholarly journals In vivo study and thermodynamic investigation of two lanthanum complexes, La(dpp)3 and La(XT), for the treatment of bone resorption disorders

2015 ◽  
Vol 6 (11) ◽  
pp. 6439-6447 ◽  
Author(s):  
J. F. Cawthray ◽  
D. M. Weekes ◽  
O. Sivak ◽  
A. L. Creagh ◽  
F. Ibrahim ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Ex Vivo ◽  
In Vivo Study ◽  
Oral Drug ◽  
Thermodynamic Investigation ◽  
Preventative Measure ◽  
Drug Candidates

Lanthanum could act as a preventative measure against bone resorption disorders; two compounds are thoroughly investigated both in vivo and ex vivo as potential oral drug candidates.

scholarly journals hPSC-Derived Enteric Ganglioids Model Human ENS Development and Function

2022 ◽  
Author(s):  
Homa Majd ◽  
Ryan M Samuel ◽  
Jonathan T Ramirez ◽  
Ali Kalantari ◽  
Kevin Barber ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Xenograft Model ◽  
Developmental Relationships ◽  
Drug Candidates ◽  
Effective Strategies ◽  
Wide Range ◽  
Functional Features ◽  
And Function

The enteric nervous system (ENS) plays a central role in gut physiology and mediating the crosstalk between the gastrointestinal (GI) tract and other organs. The human ENS has remained elusive, highlighting the need for an in vitro modeling and mapping blueprint. Here we map out the developmental and functional features of the human ENS, by establishing robust and scalable 2D ENS cultures and 3D enteric ganglioids from human pluripotent stem cells (hPSCs). These models recapitulate the remarkable neuronal and glial diversity found in primary tissue and enable comprehensive molecular analyses that uncover functional and developmental relationships within these lineages. As a salient example of the power of this system, we performed in-depth characterization of enteric nitrergic neurons (NO neurons) which are implicated in a wide range of GI motility disorders. We conducted an unbiased screen and identified drug candidates that modulate the activity of NO neurons and demonstrated their potential in promoting motility in mouse colonic tissue ex vivo. We established a high-throughput strategy to define the developmental programs involved in NO neuron specification and discovered that PDGFR inhibition boosts the induction of NO neurons in enteric ganglioids. Transplantation of these ganglioids in the colon of NO neuron-deficient mice results in extensive tissue engraftment, providing a xenograft model for the study of human ENS in vivo and the development of cell-based therapies for neurodegenerative GI disorders. These studies provide a framework for deciphering fundamental features of the human ENS and designing effective strategies to treat enteric neuropathies.  

Assessment of the osteogenic potential of alendronate on isolated adipose-derived stem cells: An ex-vivo and in-vivo study

2015 ◽  
Vol 10 (1) ◽  
pp. 32
Author(s):  
MarwaM Ellithy ◽  
MohamedS Ayoub ◽  
EffatA Abbas ◽  
MohamedA Abd El Hamid ◽  
HouryM Baghdadi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Ex Vivo ◽  
In Vivo Study ◽  
Osteogenic Potential ◽  
Adipose Derived Stem Cells

scholarly journals Influence of extracorporeal membrane oxygenation on the pharmacokinetics of ceftolozane/tazobactam: an ex vivo and in vivo study

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Camille Mané ◽  
Clément Delmas ◽  
Jean Porterie ◽  
Géraldine Jourdan ◽  
Patrick Verwaerde ◽  
...  
Keyword(s):  
Extracorporeal Membrane Oxygenation ◽  
Ex Vivo ◽  
In Vivo Study ◽  
Membrane Oxygenation

scholarly journals New device permitting non‐invasive reversal of fetal endoscopic tracheal occlusion: ex‐vivo and in‐vivo study

2020 ◽  
Vol 56 (4) ◽  
pp. 522-531 ◽  
Author(s):  
D. Basurto ◽  
N. Sananès ◽  
E. Verbeken ◽  
D. Sharma ◽  
E. Corno ◽  
...  
Keyword(s):  
Ex Vivo ◽  
In Vivo Study ◽  
Tracheal Occlusion ◽  
New Device ◽  
Non Invasive

scholarly journals Brain Tissue Conductivity Measurements with MR-Electrical Properties Tomography: An In Vivo Study

2020 ◽  
Author(s):  
Stefano Mandija ◽  
Petar I. Petrov ◽  
Jord J. T. Vink ◽  
Sebastian F. W. Neggers ◽  
Cornelis A. T. van den Berg
Keyword(s):  
Electrical Properties ◽  
Healthy Subjects ◽  
Ex Vivo ◽  
Simulated Data ◽  
Ground Truth ◽  
In Vivo Study ◽  
Conductivity Measurements ◽  
Tissue Conductivity ◽  
Before And After

AbstractFirst in vivo brain conductivity reconstructions using Helmholtz MR-Electrical Properties Tomography (MR-EPT) have been published. However, a large variation in the reconstructed conductivity values is reported and these values differ from ex vivo conductivity measurements. Given this lack of agreement, we performed an in vivo study on eight healthy subjects to provide reference in vivo brain conductivity values. MR-EPT reconstructions were performed at 3 T for eight healthy subjects. Mean conductivity and standard deviation values in the white matter, gray matter and cerebrospinal fluid (σWM, σGM, and σCSF) were computed for each subject before and after erosion of regions at tissue boundaries, which are affected by typical MR-EPT reconstruction errors. The obtained values were compared to the reported ex vivo literature values. To benchmark the accuracy of in vivo conductivity reconstructions, the same pipeline was applied to simulated data, which allow knowledge of ground truth conductivity. Provided sufficient boundary erosion, the in vivo σWM and σGM values obtained in this study agree for the first time with literature values measured ex vivo. This could not be verified for the CSF due to its limited spatial extension. Conductivity reconstructions from simulated data verified conductivity reconstructions from in vivo data and demonstrated the importance of discarding voxels at tissue boundaries. The presented σWM and σGM values can therefore be used for comparison in future studies employing different MR-EPT techniques.

751 Prediction of Colonic Neoplasia Through Spectral Marker Analysis from the Endoscopically Normal Rectum: An Ex Vivo and In Vivo Study

2008 ◽  
Vol 134 (4) ◽  
pp. A-109
Author(s):  
Hemant K. Roy ◽  
Vladimir Turzhitsky ◽  
Andrew Gomes ◽  
Michael J. Goldberg ◽  
Jeremy D. Rogers ◽  
...  
Keyword(s):  
Ex Vivo ◽  
In Vivo Study ◽  
Marker Analysis ◽  
Colonic Neoplasia

scholarly journals Role of the captured retroviral envelope syncytin-B gene in the fusion of osteoclast and giant cell precursors and in bone resorption, analyzed ex vivo and in vivo in syncytin-B knockout mice

Bone Reports ◽  
2019 ◽  
Vol 11 ◽  
pp. 100214 ◽  
Author(s):  
Amélie E. Coudert ◽  
François Redelsperger ◽  
Yasmine Chabbi-Achengli ◽  
Cécile Vernochet ◽  
Caroline Marty ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Giant Cell ◽  
Knockout Mice ◽  
Ex Vivo

scholarly journals Mechanism in Inhibitory Effects of Vitamin K2 on Osteoclastic Bone Resorption: In Vivo Study in Osteopetrotic (op/op) Mice.

1999 ◽  
Vol 45 (4) ◽  
pp. 501-507 ◽  
Author(s):  
Toshitsugu KAWATA ◽  
Joseph H. ZERNIK ◽  
Tadashl FUJITA ◽  
Chiyoko TOKIMASA ◽  
Kazuo TANNE
Keyword(s):  
Bone Resorption ◽  
Vitamin K2 ◽  
Osteoclastic Bone Resorption ◽  
In Vivo Study ◽  
Inhibitory Effects

scholarly journals Ex vivo and in vivo study of Kowa HA-2 applanation tonometer in the measurement of intraocular pressure in cats

2017 ◽  
Vol 38 (6) ◽  
pp. 3647
Author(s):  
Claudia Lizandra Ricci ◽  
Rogério Giuffrida ◽  
Glaucia Prada Kanashiro ◽  
Hilidia Stephania Rufino Belezzi ◽  
Carolina De Carvalho Bacarin ◽  
...  
Keyword(s):  
Intraocular Pressure ◽  
Ex Vivo ◽  
Clinical Signs ◽  
In Vivo Study ◽  
Eye Drops ◽  
Significant Difference ◽  
Applanation Tonometer ◽  
Ex Vivo Study ◽  
Healthy Eyes

The objective of this study was to evaluate the use of the Kowa HA-2 applanation tonometer in measuring intraocular pressure (IOP) in cats. Ten healthy eyes were used in an ex vivo study in which the calibration curve for manometry vs. tonometry was determined by artificially raising the IOP in 5 mmHg increments up to 60 mmHg (10-60 mmHg). Both eyes of 10 anesthetized cats were studiedin vivo to compare manometry vs. tonometry. In the ambulatory study, 78 healthy eyes, 7 eyes with glaucoma and 20 eyes with uveitis were evaluated by tonometry, which was performed with topical anesthesia and 1% fluorescein eye drops for the formation of fluorescein semicircles. The correlation coefficient (r²) between the manometer and the Kowa HA-2 tonometer was 0.993 and the linear regression equation was y = 0.0915x + 0.0878 in the ex-vivo study. In the in vivo study, the IOP values (mean±SD, in mmHg) in manometry were 15.6 ± 1.1(14.0 – 17.5) and in tonometry were 15.5 ± 1.2(13.5 – 17.2), with no significant difference (P > 0.05) between manometry and tonometry. In ambulatory study, using the Kowa HA-2 tonometer, the IOP values (mean±SD, in mmHg) were 15.0 ± 1.5 (11.8 – 18.3) for the healthy eyes, 38.4 ± 8.1(29.6 – 53.7) for glaucomatous eyes and 10.4 ± 2.0(5.3 – 12.2) for eyes with uveitis. There was a strong correlation and accuracy between the IOP values with the manometry and the Kowa HA-2 tonometer. In the ambulatorystudy the IOP values obtained with the tonometer were compatible for animals with healthy eyes and with clinical signs of glaucoma and uveitis. We conclude that the Kowa HA-2 tonometer can be used in the measurement of IOP in cats, since it is a practical and accurate method in this species.

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