Enhanced biogas production from wheat straw with the application of synergistic microbial consortium pretreatment

RSC Advances ◽  
2016 ◽  
Vol 6 (65) ◽  
pp. 60187-60195 ◽  
Author(s):  
Chao Zhong ◽  
Chunming Wang ◽  
Fengxue Wang ◽  
Honghua Jia ◽  
Ping Wei ◽  
...  
Keyword(s):  
Wheat Straw ◽  
Lignocellulosic Biomass ◽  
Microbial Consortium ◽  
Biogas Production ◽  
Utilization Efficiency ◽  
Biomass Utilization

Pretreatment of lignocellulosic biomass by using synergistic microbial consortium is an efficient way to promote biomass utilization efficiency.

scholarly journals Effect of Cellulase-producing Microbial Consortium on Biogas Production from Lignocellulosic Biomass

2017 ◽  
Vol 141 ◽  
pp. 180-183 ◽  
Author(s):  
Prapakorn Tantayotai ◽  
Peerapong Pornwongthong ◽  
Chotika Muenmuang ◽  
Theerawut Phusantisampan ◽  
Malinee Sriariyanun
Keyword(s):  
Lignocellulosic Biomass ◽  
Microbial Consortium ◽  
Biogas Production

scholarly journals A comprehensive study of the promoting effect of manganese on white rot fungal treatment for enzymatic hydrolysis of woody and grass lignocellulose

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Xiao Fu ◽  
Jialong Zhang ◽  
Xiangyu Gu ◽  
Hongbo Yu ◽  
Shulin Chen
Keyword(s):  
Wheat Straw ◽  
Lignocellulosic Biomass ◽  
Quantum Coherence ◽  
Plant Cell Wall ◽  
White Rot ◽  
Yield Enhancement ◽  
Biomass Recalcitrance ◽  
Fungal Treatment ◽  
Ether Linkage ◽  
Glucose Yield

Abstract Background The efficiency of biological systems as an option for pretreating lignocellulosic biomass has to be improved to make the process practical. Fungal treatment with manganese (Mn) addition for improving lignocellulosic biomass fractionation and enzyme accessibility were investigated in this study. The broad-spectrum effect was tested on two different types of feedstocks with three fungal species. Since the physicochemical and structural properties of biomass were the main changes caused by fungal degradation, detailed characterization of biomass structural features was conducted to understand the mechanism of Mn-enhanced biomass saccharification. Results The glucose yields of fungal-treated poplar and wheat straw increased by 2.97- and 5.71-fold, respectively, after Mn addition. Particularly, over 90% of glucose yield was achieved in Mn-assisted Pleurotus ostreatus-treated wheat straw. A comparison study using pyrolysis gas chromatography mass spectrometry (Py-GC/MS) and two-dimensional 1H–13C heteronuclear single quantum coherence (2D HSQC) nuclear magnetic resonance (NMR) spectroscopy was conducted to elucidate the role of Mn addition on fungal disruption of the cross-linked structure of whole plant cell wall. The increased Cα-oxidized products was consistent with the enhanced cleavage of the major β-O-4 ether linkages in poplar and wheat straw lignin or in the wheat straw lignin–carbohydrate complexes (LCCs), which led to the reduced condensation degree in lignin and decreased lignin content in Mn-assisted fungal-treated biomass. The correlation analysis and principal component analysis (PCA) further demonstrated that Mn addition to fungal treatment enhanced bond cleavage in lignin, especially the β-O-4 ether linkage cleavage played the dominant role in removing the biomass recalcitrance and contributing to the glucose yield enhancement. Meanwhile, enhanced deconstruction of LCCs was important in reducing wheat straw recalcitrance. The findings provided not only mechanistic insights into the Mn-enhanced biomass digestibility by fungus, but also a strategy for improving biological pretreatment efficiency of lignocellulose. Conclusion The mechanism of enhanced saccharification of biomass by Mn-assisted fungal treatment mainly through Cα-oxidative cleavage of β-O-4 ether linkages further led to the decreased condensation degree in lignin, as a result, biomass recalcitrance was significantly reduced by Mn addition. Graphic abstract

An improved method for determining microbially available phosphorus in drinking water

2016 ◽  
Vol 16 (4) ◽  
pp. 1149-1158 ◽  
Author(s):  
Gang Wen ◽  
Qin Deng ◽  
Ting-Lin Huang ◽  
Jun Ma
Keyword(s):  
Drinking Water ◽  
Microbial Consortium ◽  
Incubation Temperature ◽  
Organic Phosphorus ◽  
High Sensitivity ◽  
Cell Number ◽  
Growth Potential ◽  
Utilization Efficiency ◽  
Available Phosphorus ◽  
Time Saving

Microbially available phosphorus (MAP) is the labile phosphorus that is readily assimilated by microorganisms, which is linearly correlated to bacterial re-growth in drinking water in some regions. The conventional MAP bioassay for drinking water was originally developed by Markku based on the growth potential of Pseudomonas fluorescens P17 (P17). However, the bioassay bears some demerits, such as time-consuming and labor-intensive enumeration. For convenience, an alternative method based on a similar principle was developed to assess the content of MAP in drinking water, in which natural microbial consortium was used as inoculum instead of pure culture P17, cell number was counted using flow cytometry (FCM), and cultivation at 30 °C was adopted. Natural microbial consortium is able to efficiently utilize organic phosphorus and exhibit high sensitivity since more cells are produced per μg P utilized. FCM is a rapid method to count all bacteria growing in drinking water. With incubation temperature increasing up to 30 °C, there is a shorter test period (64 h), excellent sensitivity and better utilization efficiency for organic phosphorus. The results show that the developed bioassay is sensitive, time-saving and easily operated.

scholarly journals Integrated Process for Ethanol, Biogas, and Edible Filamentous Fungi-Based Animal Feed Production from Dilute Phosphoric Acid-Pretreated Wheat Straw

2017 ◽  
Vol 184 (1) ◽  
pp. 48-62 ◽  
Author(s):  
Ramkumar B. Nair ◽  
Maryam M. Kabir ◽  
Patrik R. Lennartsson ◽  
Mohammad J. Taherzadeh ◽  
Ilona Sárvári Horváth
Keyword(s):  
Phosphoric Acid ◽  
Total Energy ◽  
Wheat Straw ◽  
Ethanol Fermentation ◽  
Biogas Production ◽  
Waste Stream ◽  
Energy Output ◽  
Yield Potential ◽  
Generation Process ◽  
Pretreated Wheat Straw

AbstractIntegration of wheat straw for a biorefinery-based energy generation process by producing ethanol and biogas together with the production of high-protein fungal biomass (suitable for feed application) was the main focus of the present study. An edible ascomycete fungal strain Neurospora intermedia was used for the ethanol fermentation and subsequent biomass production from dilute phosphoric acid (0.7 to 1.2% w/v) pretreated wheat straw. At optimum pretreatment conditions, an ethanol yield of 84 to 90% of the theoretical maximum, based on glucan content of substrate straw, was observed from fungal fermentation post the enzymatic hydrolysis process. The biogas production from the pretreated straw slurry showed an improved methane yield potential up to 162% increase, as compared to that of the untreated straw. Additional biogas production, using the syrup, a waste stream obtained post the ethanol fermentation, resulted in a combined total energy output of 15.8 MJ/kg wheat straw. Moreover, using thin stillage (a waste stream from the first-generation wheat-based ethanol process) as a co-substrate to the biogas process resulted in an additional increase by about 14 to 27% in the total energy output as compared to using only wheat straw-based substrates.

Diversity of a mesophilic lignocellulolytic microbial consortium which is useful for enhancement of biogas production

2012 ◽  
Vol 111 ◽  
pp. 49-54 ◽  
Author(s):  
Lei Yan ◽  
Yamei Gao ◽  
Yanjie Wang ◽  
Quan Liu ◽  
Zhiyuan Sun ◽  
...  
Keyword(s):  
Microbial Consortium ◽  
Biogas Production

scholarly journals Does Acid Addition Improve Liquid Hot Water Pretreatment of Lignocellulosic Biomass towards Biohydrogen and Biogas Production?

2020 ◽  
Vol 12 (21) ◽  
pp. 8935 ◽  
Author(s):  
George Dimitrellos ◽  
Gerasimos Lyberatos ◽  
Georgia Antonopoulou
Keyword(s):  
Lignocellulosic Biomass ◽  
Biogas Production ◽  
Hot Water ◽  
Liquid Hot Water ◽  
Acid Addition ◽  
Water Pretreatment ◽  
Inhibitory Compounds ◽  
Production Of Hydrogen ◽  
Potential Inhibitors ◽  
Hot Water Pretreatment

The effect of liquid hot water (LHW) pretreatment with or without acid addition (A-LHW) on the production of hydrogen—through dark fermentation (DF)—and methane—through anaerobic digestion (AD)—using three different lignocellulosic biomass types (sunflower straw (SS), grass lawn (GL), and poplar sawdust (PS)) was investigated. Both pretreatment methods led to hemicellulose degradation, but A-LHW resulted in the release of more potential inhibitors (furans and acids) than the LHW pretreatment. Biological hydrogen production (BHP) of the cellulose-rich solid fractions obtained after LHW and A-LHW pretreatment was enhanced compared to the untreated substrates. Due to the release of inhibitory compounds, LHW pretreatment led to higher biochemical methane potential (BMP) than A-LHW pretreatment when both separated fractions (liquid and solid) obtained after pretreatments were used for AD. The recovered energy in the form of methane with LHW pretreatment was 8.4, 12.5, and 7.5 MJ/kg total solids (TS) for SS, GL, and PS, respectively.

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